Calcineurin B-like 3 calcium sensor associates with and inhibits 5′-methylthioadenosine nucleosidase 2 in Arabidopsis

Ok, Sung Han; Cho, Joo Hyuk; Oh, Seung Ick; Choi, Mi Na; Yeon, Jae Kyung; Shin, Jeong Sheop; Kim, Kyung Nam

doi:10.1016/j.plantsci.2015.06.016

Cited by 15 publications

(15 citation statements)

References 66 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluorescent protein constructs were introduced into the onion ( Allium cepa ) and tobacco ( Nicotiana benthamiana ) epidermal cells via particle bombardment (Oh et al, 2008) and Agrobacterium infiltration (Ok et al, 2015), respectively. The plant cells transiently expressing the fluorescence proteins were analyzed with a confocal laser scanning microscope (LSM 510 META; Carl Zeiss) and Zeiss LSM 510 software (Zeiss LSM Image Examiner).…”

Section: Methodsmentioning

confidence: 99%

Ectopic Expression of SjCBL1, Calcineurin B-Like 1 Gene From Sedirea japonica, Rescues the Salt and Osmotic Stress Hypersensitivity in Arabidopsis cbl1 Mutant

et al. 2018

Self Cite

View full text Add to dashboard Cite

Extensive studies with Arabidopsis thaliana suggested that calcineurin B-like (CBL) proteins constitute a unique family of calcium sensors in plants, which mediate a variety of abiotic stress responses. However, little is known about their function in most plants that do not have available genome sequences. In this study, we have developed a pair of universal primers that make it possible to isolate CBL1-like genes from various plants without sequence information. Using these primers, we successfully cloned a full-length cDNA of CBL1-like gene in Sedirea japonica (SjCBL1). Bimolecular fluorescence complementation (BiFC) and pull-down assays demonstrated that like Arabidopsis CBL1 (AtCBL1), SjCBL1 can interacts physically with Arabidopsis CBL-interacting protein kinase 1 (AtCIPK1) at the plasma membrane of plant cells in a Ca2+-dependent manner. In addition, overexpression of SjCBL1 in the Arabidopsis cbl1 mutant resulted in not only rescuing the hypersensitive phenotype toward salt and osmotic stresses, but also substantially enhancing the tolerance to them. Taken together, these results strongly suggest that SjCBL1 is a functional ortholog of AtCBL1 in Sedirea japonica, which can play a critical role in response to salt and osmotic stresses. Therefore, it is clear that our findings should significantly contribute to broadening and deepening our understanding of the CBL1-mediated Ca2+ signaling networks in the plant kingdom.

show abstract

Section: Methodsmentioning

confidence: 99%

Ectopic Expression of SjCBL1, Calcineurin B-Like 1 Gene From Sedirea japonica, Rescues the Salt and Osmotic Stress Hypersensitivity in Arabidopsis cbl1 Mutant

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Subsequently, epidermal cells of infiltrated tobacco leaves were examined for fluorescence using the confocal laser scanning microscope. The detailed procedure has been previously reported (Ok et al, 2015). …”

Section: Methodsmentioning

confidence: 99%

“…GST-fusion proteins such as GST-CBL4, GST-CBL10-cMyc, and GST-TOC34 were purified basically according to the protocols described earlier (Ok et al, 2015). Briefly, E. coli BL21 cells possessing a GST fusion construct were cultured at 37°C overnight and were subcultured until the OD 600 reached 0.5–0.6.…”

Section: Methodsmentioning

confidence: 99%

“…Pull-down assay was performed as described previously (Ok et al, 2015). Briefly, GST fusion proteins attached to the glutathione-Sepharose 4B beads were incubated at 4°C with prey proteins lacking the GST protein in the binding buffer (50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 0.05% Tween 20, and 1 mM PMSF) supplemented with either 0.2 mM CaCl 2 or 1 mM EGTA.…”

Section: Methodsmentioning

confidence: 99%

“…This narrow spectrum of CBL targets was somewhat unexpected as compared with the target diversity exhibited by other sensor relays such as CaMs and CMLs. Recently, however, this belief has been overturned due to our previous two reports, in which we demonstrated that one of the CBL family members, CBL3, specifically interacts with and inhibits the Arabidopsis 5′-methylthioadenosine nucleosidase (AtMTAN) family members in a Ca 2+ -dependent manner (Oh et al, 2008; Ok et al, 2015). These findings raised a possibility that each of the CBL members can have distinct interaction partner proteins besides CIPKs and prompted us to seek out new proteins targeted by the other CBL family members.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis

et al. 2016

Self Cite

View full text Add to dashboard Cite

As calcium sensor relays in plants, calcineurin B-like (CBL) proteins provide an important contribution to decoding Ca2+ signatures elicited by a variety of abiotic stresses. Currently, it is well known that CBLs perceive and transmit the Ca2+ signals mainly to a group of serine/threonine protein kinases called CBL-interacting protein kinases (CIPKs). In this study, we report that the CBL10 member of this family has a novel interaction partner besides the CIPK proteins. Yeast two-hybrid screening with CBL10 as bait identified an Arabidopsis cDNA clone encoding a TOC34 protein, which is a member of the TOC (Translocon of the Outer membrane of the Chloroplasts) complex and possesses the GTPase activity. Further analyses showed that in addition to CBL10, CBL7 also interacts with TOC34 at much lower strength in the yeast two-hybrid system. However, the rest of the CBL family members failed to interact with TOC34. Bimolecular fluorescence complementation (BiFC) analysis verified that the CBL10-TOC34 interaction occurs at the outer membrane of chloroplasts in vivo. In addition, we also demonstrated that CBL10 physically associates with TOC34 in vitro, resulting in a significant decrease in the GTPase activity of the TOC34 protein. Taken together, our findings clearly indicate that a member of the CBL family, CBL10, can modulate not only the CIPK members but also TOC34, allowing the CBL family to relay the Ca2+ signals in more diverse ways than currently known.

show abstract

Genome-Wide Identification of the BvCBL Genes in Sugar Beet (Beta vulgaris L.) and Their Expression Under Salt and Drought Conditions

Xie

Wei

et al. 2022

J Plant Growth Regul

View full text Add to dashboard Cite

Calcineurin B-like 3 calcium sensor associates with and inhibits 5′-methylthioadenosine nucleosidase 2 in Arabidopsis

Cited by 15 publications

References 66 publications

Ectopic Expression of SjCBL1, Calcineurin B-Like 1 Gene From Sedirea japonica, Rescues the Salt and Osmotic Stress Hypersensitivity in Arabidopsis cbl1 Mutant

Ectopic Expression of SjCBL1, Calcineurin B-Like 1 Gene From Sedirea japonica, Rescues the Salt and Osmotic Stress Hypersensitivity in Arabidopsis cbl1 Mutant

Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis

Genome-Wide Identification of the BvCBL Genes in Sugar Beet (Beta vulgaris L.) and Their Expression Under Salt and Drought Conditions

Contact Info

Product

Resources

About