Caffeine induces cardiac and other malformations in the rat

Matsuoka, Rumiko; Uno, Hideo; Tanaka, Harumi; Kerr, Catherine; Nakazawa, Kazuharu; Nadal‐Ginard, Bernardo

doi:10.1002/ajmg.1320280548

Cited by 20 publications

(7 citation statements)

References 17 publications

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“…During prenatal period caffeine may lead to improper development of many organs causing for example delayed neural tube closure [21], craniofacial and limb malformations, cleft palate [22,23], cardiovascular system malformations [12], the thymus involution and defects of skeletal system [24].…”

Section: Discussionmentioning

confidence: 99%

Changes in thickness of each layer of developing chicken cornea after administration of caffeine.

Kujawa-Hadrys

Dariusz²,

Bartel³

2010

Folia Histochem Cytobiol.

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Abstract:The aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs were divided into two groups: control (n=30) in which Ringer liquid was given, and experimental (n=30) in which teratogenic dose of caffeine was administrated -3.5 mg/egg. In 36 th hour of incubation solutions were given with cannula through a hole in an egg shell directly onto amniotic membrane. After closing the hole with paraffin, eggs were put back into incubator. On 10 th and 19 th day of incubation corneas were taken for morphometric and morphological analysis. In experimental groups reduction of corneal thickness, thickening of corneal epithelium and corneal endothelium as well as Bowman's and Descemet's membranes, decrease of thickness of corneal stroma in comparison with the control group have been observed. Caffeine causes thickness changes of all layers and decreases the total thickness of a developing cornea.

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Section: Discussionmentioning

confidence: 99%

Changes in thickness of each layer of developing chicken cornea after administration of caffeine.

Kujawa-Hadrys

Dariusz²,

Bartel³

2010

Folia Histochem Cytobiol.

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“…Matsouka et al (1987) found cardiovascular malformations in the majority of rat foetuses after a single intraperitoneal injection of 150 mg/kg body weight caffeine. The eye lenses were examined in 10 foetuses, and degeneration was found in all.…”

Section: Types Of Malformationsmentioning

confidence: 96%

“…When pregnant rats were given a single intraperitoneal injection of 150 mg caffeine/kg body weight on days 10 to 11 of gestation, cardiovascular anomalies were found in 87.8 % of the foetuses (Matsouka et al, 1987). In rats given total doses of 30 or 60 mg caffeine/kg body weight by injection, divided into four applications, 36.5 % and 50.9 % of the foetuses, respectively, developed cardiovascular anomalies.…”

Section: Mode Of Administrationmentioning

confidence: 99%

Intake of caffeine and other methylxanthines during pregnancy and risk for adverse effects in pregnant women and their foetuses

Andersson¹,

Hallström²,

Kihlman³

2005

TemaNord

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“…Caffeine stimulates the heart, respiratory system and central nervous systems, and the occurrence of fetal loss is characterized by a linear trend on the log scale for each 100 mg ingested daily during pregnancy (Infante‐Rivard et al 1993). Indeed, Matsuoka et al 1987) demonstrated dose‐dependent teratogenic effects of caffeine on the fetal heart, detectable at relatively low concentrations. The most susceptible stage is during septation of the heart, and some extracardiovascular anomalies have been demonstrated with skeletal malformations in some fetuses.…”

Section: Introductionmentioning

confidence: 99%

Excessive maternal caffeine exposure during pregnancy is cataractogenic for neonatal crystalline lenses in rats: a biomicroscopic and histopathologic study

Evereklioğlu

Güldür

Alaşehirli

et al. 2004

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To investigate histologically the influence of maternal caffeine exposure during pregnancy in vivo on crystalline lenses in neonatal rats. Methods: Experimentally naive, female Wistar-albino rats (200-220 g) were mated with adult male rats over 2 days for copulation. After confirming pregnancy with a vaginal smear method, 50 gravid rats (dams) were randomly divided into five groups (n ¼ 10 in each), consisting of one control and four experimental groups. Groups 1, 2 and 3 experimental dams were treated with intraperitoneal (i.p.) caffeine at doses of 25, 50 and 100 mg/kg/day, respectively, during pregnancy from gestational day 9 through to day 21. Group 4 dams were treated with caffeine in distilled water in a gavage at a dose of 50 mg/kg/day. Group 5 control dams were given i.p. saline solution daily for the same period. After normal delivery, the eyes were examined by slit-lamp biomicroscopy. The neonates were then killed by decapitation at postnatal days 1 or 30 and the eyes removed for histopathologic investigation of the lenses. Results: Group 1 and control eyes had normal anterior lens capsules with a single layer of anterior cuboidal epithelial cells, regularly oriented cortical and nuclear lens fibres, and a clear posterior lens capsule with no lining epithelial cells behind the equator. In the remaining groups, histopathologic findings suggesting cataractogenesis included eosinophilic degeneration, lens fibre cell swelling and liquefaction, central lens fibres with retained nuclei, and prominent epithelial cells lining the posterior lens capsule behind the equator. Moreover, some lenses in group 3 had immature cataract on slit-lamp biomicroscopic examination at postnatal day 30. Conclusion: Excessive maternal caffeine exposure during pregnancy had cataractogenic effects on developing crystalline lenses in newborn rat eyes, both macroscopically and histopathologically. If an appropriate dose of caffeine can be identified, caffeine-induced cataract formation may be used as a new experimental cataract model in animal studies.

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Caffeine induces cardiac and other malformations in the rat

Cited by 20 publications

References 17 publications

Changes in thickness of each layer of developing chicken cornea after administration of caffeine.

Changes in thickness of each layer of developing chicken cornea after administration of caffeine.

Intake of caffeine and other methylxanthines during pregnancy and risk for adverse effects in pregnant women and their foetuses

Excessive maternal caffeine exposure during pregnancy is cataractogenic for neonatal crystalline lenses in rats: a biomicroscopic and histopathologic study

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