Abstract:Abstract:The aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs were divided into two groups: control (n=30) in which Ringer liquid was given, and experimental (n=30) in which teratogenic dose of caffeine was administrated -3.5 mg/egg. In 36 th hour of incubation soluti… Show more
“…Caffeine readily crosses the placental barrier and into the milk of nursing mothers [6], and numerous attempts have been made to assess the impact of caffeine on embryonic and foetal development. Many experimental studies on animals have shown teratogenic and embryotoxic effects of high caffeine doses.…”
Section: Discussionmentioning
confidence: 99%
“…On the basis of a number of experimental studies it has been proved that caffeine consumption during pregnancy carries a number of side-effects for both the mother and the developing foetus [2,3,4,5,6,7,8,9]. However, in the available literature, no studies have been found relating to the effects of caffeine administered at both low and high temperatures on foetal development.…”
An easy access to products containing caffeine makes it widely consumed to excess by the general population, including pregnant women. Beverages containing caffeine are consumed at different temperatures (iced, hot, room temperature). Caffeine easily passes through biological membranes, including the blood-brain barrier, the placental barrier, and can also enter the amniotic fluid, breast milk and semen. The aim of this study was to evaluate the relationship between caffeine's developmental toxicity, and the solution's temperature (both low and high) administered to pregnant female rats. Fertilized females were randomly divided into two main groups: an experimental (E) and a control group (C). The experimental groups received caffeine (30mg/day) in 10 (E 1), 25 (E 2) and 45 o C (E 3). The females in the control group were given water at the same temperature (C 1 , C 2 and C 3). On the day 21 of pregnancy, the pregnant females were killed by decapitation, using a specially prepared laboratory guillotine, after which the mothers' internal organs were weighed. Additionally, the offspring were examined using standard teratological methods. The study found that caffeine administered to pregnant females at a dose of 30mg/day and at the temperatures of 10 °C, 25 °C or 45 °C did not produce any teratogenic effects. The only sign of its adverse effect was the appearance of developmental abnormalities in the form of haematomas and saturated bleeding in the internal organs. These changes most frequently occurred in foetuses of females which received caffeine at 10 °C or 45 °C.
“…Caffeine readily crosses the placental barrier and into the milk of nursing mothers [6], and numerous attempts have been made to assess the impact of caffeine on embryonic and foetal development. Many experimental studies on animals have shown teratogenic and embryotoxic effects of high caffeine doses.…”
Section: Discussionmentioning
confidence: 99%
“…On the basis of a number of experimental studies it has been proved that caffeine consumption during pregnancy carries a number of side-effects for both the mother and the developing foetus [2,3,4,5,6,7,8,9]. However, in the available literature, no studies have been found relating to the effects of caffeine administered at both low and high temperatures on foetal development.…”
An easy access to products containing caffeine makes it widely consumed to excess by the general population, including pregnant women. Beverages containing caffeine are consumed at different temperatures (iced, hot, room temperature). Caffeine easily passes through biological membranes, including the blood-brain barrier, the placental barrier, and can also enter the amniotic fluid, breast milk and semen. The aim of this study was to evaluate the relationship between caffeine's developmental toxicity, and the solution's temperature (both low and high) administered to pregnant female rats. Fertilized females were randomly divided into two main groups: an experimental (E) and a control group (C). The experimental groups received caffeine (30mg/day) in 10 (E 1), 25 (E 2) and 45 o C (E 3). The females in the control group were given water at the same temperature (C 1 , C 2 and C 3). On the day 21 of pregnancy, the pregnant females were killed by decapitation, using a specially prepared laboratory guillotine, after which the mothers' internal organs were weighed. Additionally, the offspring were examined using standard teratological methods. The study found that caffeine administered to pregnant females at a dose of 30mg/day and at the temperatures of 10 °C, 25 °C or 45 °C did not produce any teratogenic effects. The only sign of its adverse effect was the appearance of developmental abnormalities in the form of haematomas and saturated bleeding in the internal organs. These changes most frequently occurred in foetuses of females which received caffeine at 10 °C or 45 °C.
“…Al-Saleh et al [36] found that when caffeine levels in serum increased, the number of eggs decreased, which could be connected with the accumulation of caffeine in the follicular fluid. Kujawa-Hadryś et al [37,38] showed that caffeine causes disturbances in the developing cornea in chicken embryos. Additionally, KujawaHadryś et al [38] found that caffeine causes changes to the collagen fibers of Bowman's membrane patterns and to the corneal stroma.…”
Abstract:Caffeine is a methylxanthine which permeates the placenta. In studies on animals, it has been shown to produce teratogenic and embryotoxic effects in large doses. The objective of this study was to assess the influence of caffeine on the development of bone tissue, with particular reference to elemental bone composition using an X-ray microprobe. The research was conducted on rats. The fertilized females were randomly divided into an experimental and a control group. The experimental group was given caffeine orally in 30 mg/day doses from the 8 th to the 21 st day of pregnancy, while the control group was given water. The fetuses were used to assess the growth and mineralization of the skeleton. On the basis of double dyeing, a qualitative analysis of the bone morphology and mineralization was conducted. For calcium and potassium analysis, an X-ray microprobe was used. In 67 fetuses from the experimental group, changes in skeleton staining with the alcian-alizarin method were noticed. The frequency of the development of variants in the experimental group was statistically higher. In the experimental group, a significant decrease in the calcium level, as well as an increase in the potassium level, was observed. The X-ray microprobe's undoubted advantage is that is offers a quick qualitative and quantitative analysis of the elemental composition of the examined samples. Employing this new technique may furnish us with new capabilities when investigating the essence of the pathology process.
“…Since then, a number of studies have confirmed the negative effect of high doses of caffeine ingested during pregnancy both for the mother and the developing foetus [2,3,7,15,16,18,22,25,27,30,31].…”
Background: Caffeine is one of the most frequently ingested (at various tempe-
Materials and methods: The research was conducted on rats. The fertilised females were randomly divided into two main groups: an experimental (E) and a control group (C). The experimental groups received caffeine (30 mg/day) in 10 (E1), 25 (E2) and 45°C (E3). The females in the control group were given water at the same temperature (C 1 , C 2 and C 3 ). On the 21 st day of pregnancy, the pregnant females were killed by decapitation using a specially prepared laboratory guillotine and were assessed morphometric parameters of foetuses.Results and Conclusions: Based on this work showed that: the embryotoxic effect of caffeine was only confined to a reduction in the number of offspring; the greatest changes in the morphometric parameters occurred in foetuses whose mothers received caffeine at 10°C; in the control groups, the greatest changes were observed in foetuses whose mothers were given water at 10°C during pregnancy. (Folia Morphol 2014; 72, 3: 347-352)
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.