Caffeine – a valuable tool in excitation–contraction coupling research

Stephenson, D. George

doi:10.1113/jphysiol.2007.148270

Cited by 10 publications

(5 citation statements)

References 15 publications

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“…To examine the potential involvement of Ryr1 in Eif4a3-mediated muscle cell contraction, we treated muscle cells with the Ryr activator caffeine (Stephenson, 2008). Cell contractions were observed in 81% of Eif4a3MM cultures (n=51) following treatment with 25 mM caffeine; in Eif4a3MO muscle cultures, however, caffeine treatment did not induce cell contraction (n=32)(Fig.…”

Section: Resultsmentioning

confidence: 99%

Eif4a3 is required for accurate splicing of the Xenopus laevis ryanodine receptor pre-mRNA

Haremaki

Weinstein

2012

Developmental Biology

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The Exon Junction Complex (EJC) plays a critical role in multiple posttranscriptional events, including RNA subcellular localization, nonsense-mediated decay (NMD), and translation. We previously reported that knockdown of the EJC core component Eukaryotic initiation factor 4a3 (Eif4a3) results in full-body paralysis of embryos of the frog, Xenopus laevis. Here, we explore the cellular and molecular mechanisms underlying this phenotype. We find that cultured muscle cells derived from Eif4a3 morphants do not contract, and fail to undergo calcium-dependent calcium release in response to electrical stimulation or treatment with caffeine. We show that ryr transcripts are incorrectly spliced in Eif4a3 morphants, and demonstrate that inhibition of Xenopus Ryanodine receptor (Ryr) function similarly results in embryonic paralysis. These results suggest that the EJC mediates muscle cell function via regulation of pre-mRNA splicing during early vertebrate embryogenesis.

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Section: Resultsmentioning

confidence: 99%

Eif4a3 is required for accurate splicing of the Xenopus laevis ryanodine receptor pre-mRNA

Haremaki

Weinstein

2012

Developmental Biology

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“…In addition, caffeine has been shown to improve muscle strength (22,23,45) and explosive power (23) via 1) an enhanced Ca 2ϩ efflux from the sarcoplasmic reticulum (28), 2) a direct Ca 2ϩ -sensitizing effect on skeletal muscle (43), and 3) an increase in Na ϩ -K ϩ -ATPase activity resulting from decreased serum levels of K ϩ (39). Direct ergogenic effects can also be found within the skeletal muscle after caffeine supplementation (14,40) [see review for more details (41,43)]. Moreover, caffeine is similar in structure to adenosine, and it has been proposed that it is able to inhibit the effect of adenosine on the central nervous system (CNS) by blocking the perception of pain that is propagated from the peripheral nerves to the CNS via adenosine receptors.…”

Section: Introductionmentioning

confidence: 99%

Effects of caffeine and sex on muscle performance and delayed-onset muscle soreness after exercise-induced muscle damage: a double-blind randomized trial

Chen¹,

Chen

Tung

et al. 2019

Journal of Applied Physiology

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The present study aims to investigate effects of caffeine ingestion and sex difference on muscle performance, delayed-onset muscle soreness (DOMS), and various biomarkers under exercise-induced muscle damage (EIMD). Twenty (10 male and 10 female) healthy elite college athletes were recruited. Participants ingested either caffeine (6 mg/kg) or a placebo in a randomized, double-blind, and counterbalanced fashion at 24 and 48 h following EIMD. Muscle performance, DOMS, and blood samples were taken an hour before and an hour after supplementation. Caffeine ingestion restored impaired maximal voluntary isometric contractions (MVIC: 10.2%; MVICpost: 7.2%, both P < 0.05) during EIMD across both sexes. Following caffeine ingestion during MVIC, while affected by EIMD, an interaction was found in DOMS and serum K+ (both P < 0.05), with males showing greater attenuation (21.5 and 16.9%, respectively) compared with females (4.6 and 1.3%, respectively). DOMS demonstrated an inverse correlation with MVIC after caffeine ingestion both overall and among male athletes ( r = −0.34 and −0.54, respectively; P < 0.05) but not among female athletes ( r = −0.11; P > 0.05) under EIMD. In addition, caffeine ingestion increased postexercise serum glucose and lactate concentrations across both sexes (both P < 0.05). This is the first study to show that male athletes, compared with female athletes, experience a greater reduction in DOMS during enhanced MVIC when caffeine was consumed, suggesting men might receive greater ergogenic effects from caffeine when affected by EIMD. Furthermore, caffeine ingestion was able to restore impaired muscle power among elite collegiate athletes across both sexes. NEW & NOTEWORTHY Exercise-induced muscle damage (EIMD) reduces anaerobic/aerobic performance and increases delayed-onset muscle soreness (DOMS) during exercise. We show that acute caffeine supplementation at a dosage of 6 mg/kg seems to facilitate recovery of anaerobic muscle power and attenuate DOMS after EIMD across both sexes. Furthermore, male athletes, compared with female athletes, when caffeine was prescribed, experience a greater reduction in DOMS with better restoration of impaired maximal voluntary isometric contractions. This suggests that male athletes might benefit from the ergogenic effect of acute caffeine supplementation after the onset of EIMD.

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“…2,3 Other studies demonstrated that caffeine delayed fatigue and increased the contrac-tile force of cardiac and skeletal muscle. [3][4][5][6][7][8] Caffeine also decreased the perception of muscular pain, perceived exertion, and the reaction time to a stimulus. 9,10 Several physiological and psychological mechanisms have been proposed through which caffeine may exert its ergogenic effects.…”

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confidence: 99%

Caffeine Does Not Augment Markers of Muscle Damage or Leukocytosis Following Resistance Exercise

Machado¹,

Koch²,

Willardson³

et al. 2010

International Journal of Sports Physiology and Performance

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Purpose:The purpose of this study was to evaluate the effects of caffeine ingestion before a resistance exercise session on markers of muscle damage (CK, LDH, ALT, AST) and leukocyte levels.Methods:Fifteen soccer athletes completed two resistance exercise sessions that differed only in the ingestion of caffeine or a placebo preworkout.Results:CK concentration increased significantly following the caffeine session (415.8 ± 62.8 to 542.0 ± 73.5) and the placebo session (411.5 ± 43.3 to 545.8 ± 59.9), with no significant differences between sessions. Similarly, LDH concentration increased significantly following the caffeine session (377.5 ± 18.0 to 580.5 ± 36.1) and the placebo session (384.8 ± 13.9 to 570.4 ± 36.1), with no significant differences between sessions. Both sessions resulted in significant increases in the total leukocyte count (caffeine = 6.24 ± 2.08 to 8.84 ± 3.41; placebo = 6.36 ± 2.34 to 8.77 ± 3.20), neutrophils (caffeine = 3.37 ± 0.13 to 5.15 ± 0.28; placebo = 3.46 ± 0.17 to 5.12 ± 0.24), lymphocytes (caffeine = 2.19 ± 0.091 to 2.78 ± 0.10; placebo = 2.17 ± 0.100 to 2.75 ± 0.11), and monocytes (caffeine = 0.53 ± 0.02 to 0.72 ± 0.06; placebo = 0.56 ± 0.03 to 0.69 ± 0.04), with no significant differences between sessions.Conclusion:Ingestion of caffeine at 4.5 mg⋅kg-1 did not augment markers of muscle damage or leukocyte levels above that which occurs through resistance exercise alone.

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Caffeine – a valuable tool in excitation–contraction coupling research

Cited by 10 publications

References 15 publications

Eif4a3 is required for accurate splicing of the Xenopus laevis ryanodine receptor pre-mRNA

Eif4a3 is required for accurate splicing of the Xenopus laevis ryanodine receptor pre-mRNA

Effects of caffeine and sex on muscle performance and delayed-onset muscle soreness after exercise-induced muscle damage: a double-blind randomized trial

Caffeine Does Not Augment Markers of Muscle Damage or Leukocytosis Following Resistance Exercise

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