Caffeic Acid Phenethyl Ester Suppresses the Induction of Eotaxin in Human Lung Fibroblast Cells

Abstract

The authors suggest that CAPE is a promising agent in controlling eosinophils influx in human airway.

Caffeic acid phenethyl ester suppresses eotaxin secretion and nuclear p-STAT6 in human lung fibroblast cells

“…We used IL-13 plus TNF-a as stimulator for eotaxin secretion in the same lung fibroblast cell line (CCD-11Lu) in our previous study. 22 IL-4 and IL-13 did share many similar Figure 5. The effects of CAPE on the STAT6 DNA binding activity in nuclear extracts.…”

“…22 All membranes were incubated with the following antibody overnight at 4 C with continuous shaking: mouse monoclonal antihuman STAT6 antibody (1:250 BD biosciences) and mouse monoclonal antihuman phosphorylated STAT6 (p-STAT6) (pY641) antibody (1:500 Abcam, Cambridge, UK). We detected proliferating cell nuclear antigen by mouse monoclonal antihuman antibody (1:5,000 Abcam) as internal control.…”

“…17 Data showed that both IL-4 and TNF-a require STAT6 as mediator to activate eotaxin gene expression in fibroblast cells. 19 In our previous study, 22 we demonstrated that CAPE can inhibit eotaxin secretion in human lung fibroblast (CCD11Lu cell line) stimulated synergistically by IL-13 and TNF-a. We also showed that CAPE was able to inhibit NF-kB activation.…”

Cell Penetrating Peptide Derived from Human Eosinophil Cationic Protein Decreases Airway Allergic Inflammation

“…It is associated with induction of Th2 differentiation as well as allergen specific IgE secretion by B cells 3 . In addition, IL-4 increases eotaxin expression through activation of Janus family tyrosine kinases and phosphorylation of signal transducer and activator of transcription (STAT), hence promotes eosinophilic inflammation by inducing eosinophil chemotaxis 4 .…”

Propolis as anti-inflammatory and anti-allergic compounds: Which role for flavonoids?

“…Caffeic acid is commonly present in propolis together with flavonoids [8]. CAPE is able to suppress the induction of eotaxin in human lung fibroblast cells [9] and eotaxin is a potent chemoattractant for eosinophils, basophils and T-helper type 2 lymphocytes but, anyway, this mechanism cannot be suited to a purified mast cell line in the absence of other cell types. This compound is able to inhibit directly some kinases involved in the allergic response, such as Fyn kinase [10].…”