Caffeic acid phenethyl ester activates pro-apoptotic and epithelial–mesenchymal transition-related genes in ovarian cancer cells A2780 and A2780cis

Abstract

Ovarian cancer is a highly aggressive pathology, displaying a poor prognosis and chemoresistance to classical therapy. The present study was conducted to evaluate the effect of caffeic acid phenethyl ester (CAPE) on survival of ovarian cancer cell lines, A2780 (sensitive to cisplatin) and A2780cis (resistant to cisplatin). MTT assay was used to evaluate cell viability, while the apoptotic processes were examined by flow cytometry and qRT-PCR. A reduction of cell proliferation and activation of the apoptosis was observed in both cell lines. qRT-PCR evaluation demonstrated the activation of the pro-apoptotic genes (BAD, CASP8, FAS, FADD, p53) in both cell lines. The limited therapeutic effect in A2780 cells is explained by the activation of epithelial-mesenchymal transition-related genes (ZEB1, ZEB2, or TGFBB1) as displayed by Ingenuity Network analysis. Overall data suggest that CAPE can be used as an alternative in sensitizing cells to chemotherapy.

Salidroside induces apoptosis in human ovarian cancer SKOV3 and A2780 cells through the p53 signaling pathway

“…Furthermore, p53 can influence apoptosis by regulating Bcl-2 (33). Previous studies have revealed that >50% of tumors are associated with p53 gene mutation; wild-type p53 gene therapy has been suggested to strengthen sensitivity to cisplatin in SKOV3 cells (34)(35)(36). In the present study, it was demonstrated that salidroside has the ability to induce OC apoptosis.…”

Alchemilla vulgaris agg. (Lady's mantle) from central Balkan: antioxidant, anticancer and enzyme inhibition properties

“…Namely, it is well recognized that both caffeic and gallic acid, that are present in EA extract, possess strong anti-proliferative and cytotoxic properties in wide range of tumor types such as those used in this study (breast, cervical, ovarian, prostate carcinoma). [51][52][53] These phenolic acids affected main signaling pathways involved in cell cycle progression and cell death. Moreover, they interfered with migrative and invasive properties, epithelial/ mesenchimal transition and even angiogenesis.…”

Dietary Intervention by Phytochemicals and Their Role in Modulating Coding and Non-Coding Genes in Cancer

“…CAPE has multiple biologic active properties among which we can list: antiviral, antibacterial, antioxidant, anti-inflammatory and overall anti-cancer activities [68,69,70]. CAPE is targeting the NFκB transcription factor, promoting apoptosis in a wide range of cell lines [71,72,73,74]. For example, HL-60 cells of human leukemia subjected to the CAPE treatment revealed increased apoptosis by activation of different regulatory elements caspase-3 and BAX regulator and suppression Bcl-2 [69].…”

“…CAPE was demonstrated to act as a biomarker with active role in chemoprevention and chemotherapy in oral cancer patients inhibiting Akt signalling, cell cycle regulatory proteins and NFκB function [78,79]. It has been demonstrated that CAPE has potential application in cervical cancer [80] or ovarian cancer also [74]. …”