Salidroside induces apoptosis in human ovarian cancer SKOV3 and A2780 cells through the p53 signaling pathway

Abstract

Abstract. Salidroside is one of the most potent compounds extracted from the plant Rhodiola rosea, and its cardiovascular protective effects have been studied extensively. However, the role of salidroside in human ovarian carcinoma remains unknown. The aim of the current study was to investigate the effects of salidroside on the proliferation and apoptosis of SKOV3 and A2780 cells using MTT assay and acridine orange/ethidium bromide staining. Salidroside activated caspase-3 and upregulated the levels of apoptosis-inducing factor, Bcl-2-associated X and Bcl-2-associated death promoter (Bad) proteins. Furthermore, salidroside downregulated the levels of Bcl-2, p-Bad and X-linked inhibitor of apoptosis proteins. Salidroside activated the caspase-dependent pathway in SKOV3 and A2780 cells, upregulating p53, p21Cip1/Waf1 and p16 INK4a . These results suggest that the p53/p21 Cip1/Waf1 /p16INK4a pathway may serve a key function in salidroside-mediated effects on SKOV3 and A2780 cells. The current findings indicate that salidroside may be a promising novel drug candidate for ovarian cancer therapy.

Loss of GINS2 inhibits cell proliferation and tumorigenesis in human gliomas

“…The Wnt signaling pathway, related to the occurrence, proliferation, invasion, and transference of tumors, has great potential as both a marker for tumor diagnosis and target for cancer treatments . Besides, other pathways, such as the P53 signaling pathway, are also highly connected with tumor development . Therefore, in order to verify these bioinformatics analysis results, we further assessed relevant cell cycle signaling pathways to determine their connection with GINS2.…”

“…36,37 Besides, other pathways, such as the P53 signaling pathway, are also highly connected with tumor development. 38,39 Therefore, in order to verify these bioinformatics analysis results, we further assessed relevant cell cycle signaling pathways to determine their connection with GINS2. Generally, the GINS2 gene and MCM2 molecule jointly participate in the DNA replication process as a "minimum complex".…”

Glycosylation of anthocyanins enhances the apoptosis of colon cancer cells by handicapping energy metabolism

“…The initiation of mitochondria-mediated apoptotic pathway by caspase-9 resulting in executing apoptosis by caspase-3 [38]. Hence, we detected the caspase-3 and caspase-9 activity using Caspase Activity Assay Kits to further explore the mechanism of apoptosis [39,40]. Figure 4a reveals the changes of caspase-3 activity in MC38 cells after exposing to BAE with different ANC concentrations for 48 h. The activity of caspase-3 increased to 132.5 ± 2.3%, 155.1 ± 3.6%, 169.4 ± 2.3% and 764.5 ± 3.0% for ANC doses of 100, 200, 500, and 1000 μM compared with the control, respectively.…”

Salidroside protected against MPP+‐induced Parkinson's disease in PC12 cells by inhibiting inflammation, oxidative stress and cell apoptosis

“…As one of the biologically active ingredients of the root of Rhodiola rosea, salidroside (SAL) has been used as a novel anti-inflammatory agent [11,12], free radical scavenger to inhibit oxidative stress [13], and antiapoptotic agent [14]. As one of the biologically active ingredients of the root of Rhodiola rosea, salidroside (SAL) has been used as a novel anti-inflammatory agent [11,12], free radical scavenger to inhibit oxidative stress [13], and antiapoptotic agent [14].…”