Caffeic acid derivatives from a hairy root culture of Lactuca virosa

Stojakowska, Anna; Malarz, Janusz; Szewczyk, Agnieszka; Kisiel, Wanda

doi:10.1007/s11738-011-0827-4

Cited by 30 publications

(29 citation statements)

References 39 publications

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“…Non-transformed root grew slowly and showed small growth rate (control of Nunsen 1.85 and Ljubljanska ledenka 3.07 times). Few studies have been performed on the analyses of hairy root growth [25,31,[35][36][37][38][39]. Similar difference in growth rate of hairy roots lettuce was observed earlier [39].…”

Section: Characterization Of Cultured Hairy Root Linesmentioning

confidence: 78%

Production of hairy root cultures of lettuce (Lactuca sativa L.)

et al. 2014

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Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs. Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS. Approximately in 96.7% cvs. Nansen and in 91.2% Ljubljanska ledenka inoculated explants produced hairy root when they were incubated on Murashige and Skoog (MS) half-strength medium without plant growth regulators. A total of 54% of all hairy root cultures expressed GUS activity. Every hairy root represented an independent transformation event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times the biomass of control root). A PCR analysis of the genomic DNA confirmed the presence of marker and target genes in 15 hairy roots examined.

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Section: Characterization Of Cultured Hairy Root Linesmentioning

confidence: 78%

Production of hairy root cultures of lettuce (Lactuca sativa L.)

et al. 2014

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“…Hairy roots of Echinacea purpurea (L.) Moench. (Abbasi et al 2007), Lactuca virosa L. (Stojakowska et al 2012) and Rhaponticum carthamoides (Willd.) Iljin (Skała et al 2015) also accumulated substantial amounts of the hydroxycinnamic acid derivatives.…”

Section: Resultsmentioning

confidence: 99%

“…Caffeic acid derivative contents in callus cultures of Asteraceae were lower than those in the corresponding root cultures. Calli of C. intybus and L. virosa accumulated 1.4 % DW and 0.7 % DW of caffeic acid derivatives, respectively (Stojakowska et al 2012;Malarz et al 2013). The present study is, to our knowledge, the first report on the occurrence of caffeoyl aldarates in in vitro-cultured plant tissues.…”

Section: Resultsmentioning

confidence: 99%

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Hydroxycinnamates from elecampane (Inula helenium L.) callus culture

2016

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Hydroalcoholic extract from Inula helenium callus tissue showed remarkable reducing capacity. An HPLC/DAD analysis revealed the presence of numerous hydroxycinnamic acid derivatives, including chlorogenic acid (5-O-CQA, 0.1 % dry weight) and 3,5-dicaffeoylquinic acid (3,5-DCQA, 0.3 % dry weight), which were among major constituents of the examined extract. Application of a hyphenated chromatographic method-UHPLC/DAD/MS n -allowed identification of sixteen compounds, derivatives of caffeic acid. Apart from the compounds commonly found in Inula sp., the plant material contained eight conjugates of caffeic acid with aldaric acid. The aldarates constituted over 50 % of the hydroxycinnamate fraction in the examined tissue. This is the first report on the occurrence of the caffeoylaldaric acids in Inula sp. and in a plant tissue culture.

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“…Chromatographic separations of phenolics were carried out at 25 o C, on a Zorbax Eclipse XDB-C18 column, 4.6 x 150 mm (Agilent Technologies, USA), as it was described earlier. 29 The compounds were semi-quantified using two point calibration curves, based on peak areas measured at 325 nm (caffeic acid, ferulic acid), 305 nm (p-coumaric acid) and 250 nm (p-hydroxybenzoic acid) (concentration range 0.02 -0.40 mg mL -1 ). The phenolic acids were identified by comparison of their retention times and UV spectra with those of authentic samples and co-chromatography with standards.…”

Section: Chromatographic Analysis Of Phenolic Acidsmentioning

confidence: 99%

Protective Effect of Philesia Magellanica (Coicopihue) From Chilean Patagonia Against Oxidative Damage

Suwalsky¹,

Avello²,

Obreque³

et al. 2015

J. Chil. Chem. Soc.

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Philesia magellanica (P. magellanica) is a plant collected in the Chilean Patagonia. Its antioxidant properties were assessed in human erythrocytes exposed in vitro to oxidative stress induced by HClO. Scanning electron microscopy (SEM) observations showed that HClO induced a morphological alteration in the red blood cells from a normal discoid to a spherocytic form, and cells of unequal size. However, a concentration as low as 1 mM gallic acid equivalents (GAE) of P. magellanica aqueous extract neutralized the change effects of 50 µM HClO. On the other hand, 20 µM (GAE) of the extract considerably reduced the deleterious capacity of 0.25 mM HClO to induce hemolysis in red blood cells. In addition, X-ray diffraction experiments were performed in molecular models of the human erythrocyte membrane. These consisted in multilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), classes of lipids preferentially located in the outer and inner monolayers, respectively of the human erythrocyte membrane. It was observed that P. magellanica only interacted with DMPC affecting its multilayer structure. It was also observed that 0.1 µM (GAE) of P. magellanica neutralized the structural perturbation induced by 0.05 and 0.5 mM HClO. These experiments confirmed the antioxidant properties of P. magellanica aqueous extracts.

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Caffeic acid derivatives from a hairy root culture of Lactuca virosa

Cited by 30 publications

References 39 publications

Production of hairy root cultures of lettuce (Lactuca sativa L.)

Production of hairy root cultures of lettuce (Lactuca sativa L.)

Hydroxycinnamates from elecampane (Inula helenium L.) callus culture

Protective Effect of Philesia Magellanica (Coicopihue) From Chilean Patagonia Against Oxidative Damage

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