Caenorhabditis elegans Responses to Specific Steroid Hormones.

Tominaga, Nobuaki; Ura, Kazuhiro; Kawakami, Masato; Kawaguchi, Tomoaki; Kohra, Shinya; Mitsui, Yoshinori; Iguchi, Taisen; Arizono, Koji

doi:10.1248/jhs.49.28

Cited by 20 publications

(9 citation statements)

References 22 publications

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“…21) In that experiment, C. elegans were exposed to various steroids on an agar plate. In the present paper, we investigated the applicability of our test system to low solubility or suspected endocrine disrupting chemicals.…”

Section: Resultsmentioning

confidence: 99%

A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

Tominaga

Kohra

Iguchi

et al. 2003

JOURNAL OF HEALTH SCIENCE

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The nematode Caenorhabditis elegans (C. elegans) was used for a multiple-generation toxicity bioassay of phenols. We examined the sublethal toxicity (fecundity and reproduction) of bisphenol A (BisA), 4-(1-adamantyl) phenol (Adp), and 4,4′-(1,3-adamantanediyl) diphenol (AdDP) over five generations using a Nematode Growth Medium (NGM) 1.7% agar plate. In the fourth generation, the phenols affected the fecundity rate of C. elegans at doses 100-to 10000-fold lower than the LC 50 s. In particular, at 1 nM BisA, the number of worms decreased significantly to about 50% of control (p < 0.05). A comparison of the number of viable worms and eggs suggested that the phenols exert hatching toxicity. In addition, individuals with an abnormality in the vulva, which could not ovulate, appeared on the phenol-containing plates. We confirmed that phenols disrupt reproduction in C. elegans and that the method using NGM agar plates facilitates multi-generational toxicity tests of chemicals that are poorly soluble in water.

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Section: Resultsmentioning

confidence: 99%

A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

Tominaga

Kohra

Iguchi

et al. 2003

JOURNAL OF HEALTH SCIENCE

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“…This assumption is based on (1) the absence of some key reactions of the ''classical'' pathways in many instances (e.g., Swevers et al 1991a, b;Lafont 1997), (2) the evidence for some particular metabolic transformations of exogenous ''precursors'' (although nonspecific detoxification reactions cannot be excluded at the moment), (3) the presence of yet unidentified steroids in addition to the ''classical'' ones (e.g., Ollevier et al 1986), and (4) recent accumulating data from the nematode Caenorhabditis elegans showing with genetic tools the involvement of steroids in the control of molting and longevity (Kurzchalia and Ward 2003;Matyash et al 2004). It is noteworthy that both exogenous ecdysteroids and vertebrate-type steroids affect nematode molting and/or reproductive processes (e.g., Chitwood 1999;Tominaga et al 2003), but endogenously produced steroids (dafachronic acids Fig. 2-5) that have recently identified are in fact unrelated to them Rottiers et al 2006).…”

Section: Biosynthesismentioning

confidence: 99%

Steroids in aquatic invertebrates

2007

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Steroid molecules are present in all invertebrates, and some of them have established hormonal roles: this is the case for ecdysteroids in arthropods and, to a lesser extent, for vertebrate-type steroids in molluscs. Steroids are not only hormones, they may also fulfill many other functions in chemical communication, chemical defense or even digestive physiology. The increasing occurrence of endocrine disruption problems caused by environmental pollutants, which interfere in particular with reproductive physiology of vertebrates but also of invertebrates has made necessary to better understand the endocrine physiology of the latter and the role of steroids in these processes. So many attempts are being made to better understand the endocrine roles of steroids in arthropods and molluscs, and to establish whether they also fulfill similar functions in other invertebrate phyla. At the moment, both the precise identification of these steroids, the determination of their origin (endogenous versus exogenous) and of their mechanism of action are under active investigation. This research takes profit of the development of genome sequencing programs on many invertebrate species, which allow the identification of receptors and/or biosynthetic enzymes, when related to their vertebrate counterparts, but the story is not so simple, as will be exemplified by estrogen receptors of molluscs.

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“…The androgens 17a-methyltestosterone and fenarimol did not show an effect up to 1.0 lM (Weltje et al 2003), but in the study of Tominaga et al (2003b) a concentration of 5.0 lM methyltestosterone significantly reduced nematode fecundity. The androgen trenbolone showed inhibiting effects on reproduction (0.2 nM) and stimulating effects on growth (20 nM) of Fig.…”

Section: Effects Of Potential Edcs On Nematodesmentioning

confidence: 99%

Endocrine disruption in nematodes: effects and mechanisms

Höss¹,

Weltje

2007

Ecotoxicology

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This paper reviews the current knowledge on endocrine disruption in nematodes. These organisms have received little attention in the field of ecotoxicology, in spite of their important role in aquatic ecosystems. Research on endocrine regulation and disruption in nematodes, especially the more recent studies, concentrate mainly on one species, Caenorhabditis elegans. Although an endocrine system is not known in nematodes, there is evidence that many processes are regulated via hormonal pathways. As vertebrate hormones, such as steroids, may have endocrine functions in nematodes as well, endocrine disrupting chemicals (EDCs) defined for vertebrates may also be able to influence nematodes. The studies that are reviewed here, and own data showed that potential EDCs can affect nematodes on all organizational levels, from molecules to communities. It is concluded that nematodes, notably its prominent species C. elegans, are a promising organism group for the development of biomonitoring tools, provided that more mechanistic evidence is gathered on hormonal processes within these animals.

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Caenorhabditis elegans Responses to Specific Steroid Hormones.

Cited by 20 publications

References 22 publications

A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

Steroids in aquatic invertebrates

Endocrine disruption in nematodes: effects and mechanisms

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