Cadmium versus copper toxicity: Insights from an integrated dissection of protein synthesis pathway in the digestive glands of mussel Mytilus galloprovincialis

Pytharopoulou, Sofia; Kournoutou, Georgia G.; Leotsinidis, Michalis; Georgiou, Christos D.; Kalpaxis, Dimitrios L.

doi:10.1016/j.jhazmat.2013.05.031

Cited by 23 publications

(16 citation statements)

References 57 publications

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“…On the contrary, we found that genes in aminoacyl-tRNA biosynthesis pathway are down-regulated. This result is consistent with the finding that shows protein synthesis pathway is negatively affected when mussels are exposed to heavy metal contamination [15] . We hope that the gill transcriptome and those copper inducible transcripts of hard-shelled mussel can provide some insight into the understanding of genome-wide transcriptome profile of and give useful information in fish gonad development.…”

Section: Experimental Design Materials and Methodssupporting

confidence: 93%

Transcriptome response to copper heavy metal stress in hard-shelled mussel ( Mytilus coruscus )

Jiang

Shen

et al. 2016

Genomics Data

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The hard-shelled mussel (Mytilus coruscus) has considerably one of the most economically important marine shellfish worldwide and considered as a good invertebrate model for ecotoxicity study for a long time. In the present study, we used Illumina sequencing technology (HiSeq2000) to sequence, assemble and annotate the transcriptome of the hard-shelled mussel which challenged with copper pollution. A total of 21,723,913 paired-end clean reads (NCBI SRA database SRX1411195) were generated from HiSeq2000 sequencer and 96,403 contigs (with N50 = 1118 bp) were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 1156 unigenes are upregulated and 1681 unigenes are downregulated when challenged with copper. By KEGG pathway enrichment analysis, we found that unigenes in four KEGG pathways (aminoacyl-tRNA biosynthesis, apoptosis, DNA replication and mismatch repair) show significant differential expressed between control and copper treated groups. We hope that the gill transcriptome in copper treated hard-shelled mussel can give useful information to understand how mussel handles with heavy metal stress at molecular level.

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Section: Experimental Design Materials and Methodssupporting

confidence: 93%

Transcriptome response to copper heavy metal stress in hard-shelled mussel ( Mytilus coruscus )

Jiang

Shen

et al. 2016

Genomics Data

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“…The effect on protein content was revealed after exposure of aquatic species from different trophic levels to metal copper (Pytharopoulou et al, 2013), to Metolachlor (Martins et al, 2011), to triazine herbicides (El-Sheekh et al, 1994) and to the combination of the metal copper and chloroacetanilide herbicide applied to phytoplankton species (Lu et al, 2015). The latter is in agreement with our result that the metal-herbicide mixture significantly predicted protein content of the diatom species.…”

Section: The Effect Of the Chemical Mixture On The Quality Of The Diasupporting

confidence: 89%

Effects of a herbicide and copper mixture on the quality of marine plankton

Filimonova

Troch

Gonçalves

et al. 2018

Ecotoxicology and Environmental Safety

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Pesticides and metals are often used in agriculture and are therefore often simultaneously discharged to nearby estuarine and marine areas. The effects of this organic-inorganic chemical mixture on food quality of aquatic organisms are currently unknown. In this study we test if a mixture of copper (inorganic) and the herbicide Primextra® Gold TZ (organic) affects the quality of the diatom Thalassiosira weissflogii and the copepod Acartia tonsa - two key species that fuel the local food-web. We quantified quality (i.e. energy content as food for the next trophic level) in terms of fatty acids, proteins and thiobarbituric acid reacting substances. We found non-additive effects (positive and negative) of the metal-herbicide mixture on the diatom and copepod species. In general, nutritionally important biochemical parameters of Acartia tonsa were most sensitive to the chemical stressors.

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“…Protein synthesis is critical for abiotic stress tolerance in plants [ 51 ]. Pytharopouloua et al suggested that under Cd 2+ -mediated stress, protein-synthesizing activity is reorganized both quantitatively and qualitatively, and Cu 2+ causes a progressive loss of the ability of 40S-ribosomal subunits to form the 48S pre-initiation complex, decreasing to 34 % of the control level at the end of exposure [ 52 ]. Plastid ribosomal proteins (PRPs) are essential for ribosome biogenesis, plastid protein biosynthesis, chloroplast differentiation, and early chloroplast development, and nuclear-encoded PRPS20 plays an important role in chloroplast development in rice [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

DNA microarray revealed and RNAi plants confirmed key genes conferring low Cd accumulation in barley grains

Sun

Chen

et al. 2015

BMC Plant Biol

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BackgroundUnderstanding the mechanism of low Cd accumulation in crops is crucial for sustainable safe food production in Cd-contaminated soils.ResultsConfocal microscopy, atomic absorption spectrometry, gas exchange and chlorophyll fluorescence analyses revealed a distinct difference in Cd accumulation and tolerance between the two contrasting barley genotypes: W6nk2 (a low-grain-Cd-accumulating and Cd-sensitive genotype) and Zhenong8 (a high-grain-Cd-accumulating and tolerant genotype). A DNA microarray analysis detected large-scale changes of gene expression in response to Cd stress with a substantial difference between the two genotypes. Cd stress led to higher expression of genes involved in transport, carbohydrate metabolism and signal transduction in the low-grain-Cd-accumulating genotype. Novel transporter genes such as zinc transporter genes were identified as being associated with low Cd accumulation. Quantitative RT-PCR confirmed our microarray data. Furthermore, suppression of the zinc transporter genes HvZIP3 and HvZIP8 by RNAi silencing showed increased Cd accumulation and reduced Zn and Mn concentrations in barley grains. Thus, HvZIP3 and HvZIP8 could be candidate genes related to low-grain-Cd-accumulation.ConclusionNovel transporter genes such as HvZIP3 and HvZIP8 were identified as being associated with low-grain-Cd-accumulation. In addition to advancing academic knowledge, our findings may also result in potential economic benefits for molecular breeding of low Cd accumulating barley and other crops.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0648-5) contains supplementary material, which is available to authorized users.

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Cadmium versus copper toxicity: Insights from an integrated dissection of protein synthesis pathway in the digestive glands of mussel Mytilus galloprovincialis

Cited by 23 publications

References 57 publications

Transcriptome response to copper heavy metal stress in hard-shelled mussel ( Mytilus coruscus )

Transcriptome response to copper heavy metal stress in hard-shelled mussel ( Mytilus coruscus )

Effects of a herbicide and copper mixture on the quality of marine plankton

DNA microarray revealed and RNAi plants confirmed key genes conferring low Cd accumulation in barley grains

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