Cadmium uptake and transport processes in rice revealed by stable isotope fractionation and Cd-related gene expression

Zhong, Shaobin; Li, Xiaomin; Li, Fangbai; Huang, Yujing; Liu, Tongxu; Yin, Haoming; Qiao, Jiangtao; Chen, Guojun; Huang, Fang

doi:10.1016/j.scitotenv.2021.150633

Cited by 30 publications

(33 citation statements)

References 60 publications

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“…An exception was that a hyperaccumulator plant grown on Cd contaminated soil had a heavier isotope composition than the phytoavailable pool (+0.02 to +0.18‰, Zhou et al, 2020 ). Two rice studies found that the enrichment in light isotopes was slightly enhanced in non-flooded compared to flooded soils ( Wiggenhauser et al, 2021a ; Zhong et al, 2022 ) while one found the opposite ( Zhang et al, 2021 ). Possible factors causing this difference may be distinct initial soil properties (e.g., pH and soil Cd concentration) or changes in the phytoavailability of elements, such as Mn and Fe upon flooding.…”

Section: Isotope Fractionation In Plantsmentioning

confidence: 99%

“…Plants were isotopically lighter than their phytoavailable Cd source, in hydroponic and soil studies ( Wei et al, 2018 ; Imseng et al, 2019 ; Moore et al, 2020 ; Wiggenhauser et al, 2021a ; Zhang et al, 2021 ; Zhong et al, 2021 , 2022 ; Figure 10 ). An exception was that a hyperaccumulator plant grown on Cd contaminated soil had a heavier isotope composition than the phytoavailable pool (+0.02 to +0.18‰, Zhou et al, 2020 ).…”

Section: Isotope Fractionation In Plantsmentioning

confidence: 99%

“…Several studies investigated the role of NRAMP5 on Cd isotope fractionation during uptake. In rice ( Oryza sativa ), both a small upregulation and knockout of OsNRAMP5 were associated with uptake of lighter Cd isotopes compared to control treatments ( Zhang et al, 2021 ; Zhong et al, 2022 ). This apparent discrepancy is discussed in section Genetic Approaches for Isotope Fractionation Factors.…”

Section: Isotope Fractionation In Plantsmentioning

confidence: 99%

“…This hypothesis is supported by synchrotron X-ray absorption spectroscopy (XAS) results that showed the majority of Cd in rice roots can be bound to S-containing ligands, such as glutathione or phytochelatins, which are expected to preferentially bind light Cd isotopes ( Yan et al, 2016 ; Wiggenhauser et al, 2021a ; Zhao et al, 2021 ). Alternatively, Zhong et al (2022) showed that Δ 114/110 Cd shoot-root in rice can be further impacted by the protein CAL1. This protein complexes Cd in xylem parenchyma cells and Cd-CAL1 complexes are then transported into the xylem.…”

Section: Isotope Fractionation In Plantsmentioning

confidence: 99%

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Stable Isotope Fractionation of Metals and Metalloids in Plants: A Review

et al. 2022

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This work critically reviews stable isotope fractionation of essential (B, Mg, K, Ca, Fe, Ni, Cu, Zn, Mo), beneficial (Si), and non-essential (Cd, Tl) metals and metalloids in plants. The review (i) provides basic principles and methodologies for non-traditional isotope analyses, (ii) compiles isotope fractionation for uptake and translocation for each element and connects them to physiological processes, and (iii) interlinks knowledge from different elements to identify common and contrasting drivers of isotope fractionation. Different biological and physico-chemical processes drive isotope fractionation in plants. During uptake, Ca and Mg fractionate through root apoplast adsorption, Si through diffusion during membrane passage, Fe and Cu through reduction prior to membrane transport in strategy I plants, and Zn, Cu, and Cd through membrane transport. During translocation and utilization, isotopes fractionate through precipitation into insoluble forms, such as phytoliths (Si) or oxalate (Ca), structural binding to cell walls (Ca), and membrane transport and binding to soluble organic ligands (Zn, Cd). These processes can lead to similar (Cu, Fe) and opposing (Ca vs. Mg, Zn vs. Cd) isotope fractionation patterns of chemically similar elements in plants. Isotope fractionation in plants is influenced by biotic factors, such as phenological stages and plant genetics, as well as abiotic factors. Different nutrient supply induced shifts in isotope fractionation patterns for Mg, Cu, and Zn, suggesting that isotope process tracing can be used as a tool to detect and quantify different uptake pathways in response to abiotic stresses. However, the interpretation of isotope fractionation in plants is challenging because many isotope fractionation factors associated with specific processes are unknown and experiments are often exploratory. To overcome these limitations, fundamental geochemical research should expand the database of isotope fractionation factors and disentangle kinetic and equilibrium fractionation. In addition, plant growth studies should further shift toward hypothesis-driven experiments, for example, by integrating contrasting nutrient supplies, using established model plants, genetic approaches, and by combining isotope analyses with complementary speciation techniques. To fully exploit the potential of isotope process tracing in plants, the interdisciplinary expertise of plant and isotope geochemical scientists is required.

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Section: Isotope Fractionation In Plantsmentioning

confidence: 99%

Section: Isotope Fractionation In Plantsmentioning

confidence: 99%

Section: Isotope Fractionation In Plantsmentioning

confidence: 99%

Section: Isotope Fractionation In Plantsmentioning

confidence: 99%

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Stable Isotope Fractionation of Metals and Metalloids in Plants: A Review

et al. 2022

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“…The heterologous expression of OsMTI-1b can improve the tolerance of E. coli cells to Cd by chelating metal ions [62]. Overexpression of OsMT1e improved rice growth under Cd stress [63]. After CdCl 2 treatment, Arabidopsis lines overexpressing OsMT-3a accumulated higher levels of Cd in shoots and roots [64].…”

Section: A Brief Introduction To the Mt Family Key Proteins And Chela...mentioning

confidence: 99%

Metalloprotein-Specific or Critical Amino Acid Residues: Perspectives on Plant-Precise Detoxification and Recognition Mechanisms under Cadmium Stress

Saleem

et al. 2022

IJMS

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Cadmium (Cd) pollution in cultivated land is caused by irresistible geological factors and human activities; intense diffusion and migration have seriously affected the safety of food crops. Plants have evolved mechanisms to control excessive influx of Cd in the environment, such as directional transport, chelation and detoxification. This is done by some specific metalloproteins, whose key amino acid motifs have been investigated by scientists one by one. The application of powerful cell biology, crystal structure science, and molecular probe targeted labeling technology has identified a series of protein families involved in the influx, transport and detoxification of the heavy metal Cd. This review summarizes them as influx proteins (NRAMP, ZIP), chelating proteins (MT, PDF), vacuolar proteins (CAX, ABCC, MTP), long-distance transport proteins (OPT, HMA) and efflux proteins (PCR, ABCG). We selected representative proteins from each family, and compared their amino acid sequence, motif structure, subcellular location, tissue specific distribution and other characteristics of differences and common points, so as to summarize the key residues of the Cd binding target. Then, we explain its special mechanism of action from the molecular structure. In conclusion, this review is expected to provide a reference for the exploration of key amino acid targets of Cd, and lay a foundation for the intelligent design and breeding of crops with high/low Cd accumulation.

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