Cadmium sulfate and CdTe-quantum dots alter DNA repair in zebrafish (Danio rerio) liver cells

Tang, Song; Cai, Qingsong; Chibli, Hicham; Allagadda, Vinay; Nadeau, Jay L.; Mayer, Gregory D.

doi:10.1016/j.taap.2013.06.004

Cited by 75 publications

(59 citation statements)

References 70 publications

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“…Over-expression of p53 has already been observed after Drosophila larvae exposure to Cd QDs [19]. On the contrary irregular significant expression at two higher doses of CdCl 2 was observed which would agree with the results observed in zebra fish [20]. In human breast MCF-7 cancer cells Cd 2+ exposure results in impaired p53 functions such as conformational changes, loss of DNA binding activity, down-regulation of transcriptional activity and inhibition of gamma-radiation induced DNA damage responses [21].…”

Section: Introductionsupporting

confidence: 88%

Genotoxicology of Quantum Dots Used in Medical and Pharmaceutical Sciences

Demir¹

2015

Hereditary Genet

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Section: Introductionsupporting

confidence: 88%

Genotoxicology of Quantum Dots Used in Medical and Pharmaceutical Sciences

Demir¹

2015

Hereditary Genet

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“…After the reverse transcription reaction, RT-PCR was performed by the ABI 7300 Real-Time PCR System (Applied Biosystems ® , Foster City, CA, USA). RT-PCR amplification conditions were 94°C for 10 min, followed by 40 cycles of 95°C for 15sec, and 60°C for 60sec in a 20μL reaction volume (Tang et al, 2013a;Tang et al, 2013b (Xi et al, 2007). The 2 -ΔΔCt method was used to determine the relative miRNA expression ratios of miR-193b and miR196a, in which data were normalized with RNU6 (Shen et al, 2013a).…”

Section: Rna Isolation and Quantitative Real-time Polymerase Chain Rementioning

confidence: 99%

Association of miR-193b Down-regulation and miR-196a up-Regulation with Clinicopathological Features and Prognosis in Gastric Cancer

Tang²,

Sun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…1 The cytotoxic effects of Cd-based QDs can be ascribed to the following mechanisms: degradation of the QDs and consequent release of free Cd ions (Cd 2+ ) 2-4 and generation of ROS. [5][6][7] ROS are a group of compounds endowed with high reactivity and short half-lives because of their tendency to give or receive electrons to attain stability. The three major types of ROS are H 2 O 2 , ⋅O 2 − and ⋅OH.…”

Section: Introductionmentioning

confidence: 99%

Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice

Wang

Sun

Meng

et al. 2017

IJN

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Although quantum dot (QD)-induced toxicity occurs due to free radicals, generation of oxidative stress mediated by reactive oxygen species (ROS) formation is considered an important mechanism. However, free radical mechanisms are essentially difficult to elucidate at the molecular level because most biologically relevant free radicals are highly reactive and short-lived, making them difficult to directly detect, especially in vivo. Antioxidants play an important role in preventing or, in most cases, limiting the damage caused by ROS. Healthy people and animals possess many endogenous antioxidative substances that scavenge free radicals in vivo to maintain the redox balance and genome integrity. The antioxidant capacity of an organism is highly important but seldom studied. In this study, the dose and time effects of CdTe QDs on the antioxidant capacities of the liver and kidneys were investigated in mice using the electron paramagnetic resonance (EPR) spin-trapping technique. We found that the liver and kidneys of healthy mice contain specific antioxidant capacities that scavenge ·OH and ·O 2 − . Furthermore, oxidative stress markers (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx], glutathione [GSH] and malondialdehyde [MDA]) were examined. In dose course studies, the free radical scavenging efficiencies of the liver and kidneys were found to gradually decrease with increasing concentration of CdTe QD exposure. The activities and levels of SOD, CAT, GPx and MDA were observed to increase in treated groups, whereas those of GSH were reduced. The time course studies revealed that the QD-induced antioxidant efficiency reduction was time dependent with GSH decrease and could recover after a period of time. These experimental results offer new information on QD toxicity in vivo. Specifically, CdTe QDs can deplete GSH to reduce the elimination ability of the liver and kidneys for ·OH and ·O 2 − , thus inducing oxidative damage to tissues.

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Cadmium sulfate and CdTe-quantum dots alter DNA repair in zebrafish (Danio rerio) liver cells

Cited by 75 publications

References 70 publications

Genotoxicology of Quantum Dots Used in Medical and Pharmaceutical Sciences

Genotoxicology of Quantum Dots Used in Medical and Pharmaceutical Sciences

Association of miR-193b Down-regulation and miR-196a up-Regulation with Clinicopathological Features and Prognosis in Gastric Cancer

Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice

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