Cadmium lets increase the glutathione pool in bryophytes

Bruns, I.; Sutter, Kristin; Menge, Sieglinde; Neumann, D.; Krauss, Gerhard

doi:10.1078/0176-1617-00071

Cited by 62 publications

(56 citation statements)

References 39 publications

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“…This agrees with other reports in the literature 5,11,12,13,15,16,27 which have documented the capacity of mosses to sequester heavy metals when they are in high concentration in the environment. The decrease of Cd (II) concentration observed on day 12 of the experiment is counter to the expected bio-accumulation, which suggests a possible effect of loss of efficiency or saturation of the enzymatic bioaccumulation machinery 28 .…”

Section: Determination Of CD In Bryophytessupporting

confidence: 83%

“…Previous studies with different species of mosses demonstrated that this concentration of Cadmium increased the GSH pool significantly 11,12 . Plants were sprayed, since mosses absorb water through the cuticle.…”

Section: Treatment Of Mosses With Cadmiummentioning

confidence: 99%

Section: Introductonmentioning

confidence: 99%

“…They are synthesized from glutathione, which is a tripeptide formed by glutamic acid, cysteine and glycine (γ-glutamil-cisteinilglycine). Glutathione also plays an important role in the response of plants to environmental stress, oxidative stress and that produced by xenobiotic compounds such as heavy metals 4,6,11 . Phytochelatins have been described in different types of organisms such as plants, nematodes, algae and yeasts 3,12 .…”

Section: Introductonmentioning

confidence: 99%

“…Phytochelatins have been described in different types of organisms such as plants, nematodes, algae and yeasts 3,12 . Some studies have demonstrated that bryophytes (mosses) do not form phylochelatins, but only their precursor glutathione 5,11 .Considering that bryophytes have been described as organisms which are particularly reactive to contaminants and thus may have an important role as bio-indicators of environmental contamination 5,13,14,15,16,17 , this contribution examined the presence of phytochelants in endemic Chilean mosses challenged with a heavy metal, cadmium, by means of high-pressure liquid chromatography (HPLC). …”

mentioning

confidence: 99%

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Increase of Total Glutathione as a Response to Cd Induced Stress in a Chilean Endemic Bryophytes (Thuidium Sp.)

Leinenweber

Stegen

Díaz‐Palma

2009

J. Chil. Chem. Soc.

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Bryophytes collected from the IV Region of Chile, were treated with CdCl 2 to stimulate the synthesis of phytochelants. After cadmium treatment, total peptides were extracted from the plants and were analyzed using HPLC to search for the presence of phytochelatin molecules.Chromatography results showed the absence of phytochelatin-like molecules in bryophytes. However, glutathione and a molecule suspected to be γ-glutamilcistein were detected. The latter compound is knowed to be involved in the metabolic pathway of the gluthatione and phytochelatin synthesis.The results indicated the absence of detectable phytochelatins in bryophytes containing heavy metal as contaminants. The increase in glutathione during the experimental period may have been due to a response to oxidative stress. Thus this work suggests an important role of the glutathione in the detoxification mechanisms of bryophytes.Key words: Bryophytes, glutathione, chromatography, oxidative stress. INTRODUCTONContamination is currently one of the most studied phenomena. Elevated concentrations of heavy metals, of natural or anthropogenic origin, are one of the most difficult contaminants to study 1 . However there are organisms, like a large variety of plants, which are resistant to high levels of heavy metals 2,3 . Research in these organisms resistant to heavy metals have found the presence of compounds synthesized at intra-cellular level, which are capable of immobilizing these elements. Because of their ability to store metals, these organisms have been considered as an alternative to be used as bio-monitors or biological extractors at contaminated sites 4,5,6,7 .The phytochelatins and the glutathione compose a class of compounds generated by plants in response to heavy metals. These phylochelatins are a family of peptides that have been identified in plants and some microorganisms. Phytochelatins have a primary structure of (γ-glutamil-cysteine) n glycine, with n=2-11 8,9,10 . They are synthesized from glutathione, which is a tripeptide formed by glutamic acid, cysteine and glycine (γ-glutamil-cisteinilglycine). Glutathione also plays an important role in the response of plants to environmental stress, oxidative stress and that produced by xenobiotic compounds such as heavy metals 4,6,11 . Phytochelatins have been described in different types of organisms such as plants, nematodes, algae and yeasts 3,12 . Some studies have demonstrated that bryophytes (mosses) do not form phylochelatins, but only their precursor glutathione 5,11 .Considering that bryophytes have been described as organisms which are particularly reactive to contaminants and thus may have an important role as bio-indicators of environmental contamination 5,13,14,15,16,17 , this contribution examined the presence of phytochelants in endemic Chilean mosses challenged with a heavy metal, cadmium, by means of high-pressure liquid chromatography (HPLC). EXPERIMENTAL Collection and maintenance of mossesMosses were collected from the area of Alcohuas in the IV Region of Chile (Figu...

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Section: Determination Of CD In Bryophytessupporting

confidence: 83%

Section: Treatment Of Mosses With Cadmiummentioning

confidence: 99%

Section: Introductonmentioning

confidence: 99%

Section: Introductonmentioning

confidence: 99%

mentioning

confidence: 99%

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Increase of Total Glutathione as a Response to Cd Induced Stress in a Chilean Endemic Bryophytes (Thuidium Sp.)

Leinenweber

Stegen

Díaz‐Palma

2009

J. Chil. Chem. Soc.

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Heavy Metal‐binding Proteins and Peptides

2003

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Introduction Historical Outline Chemical Structures Nomenclature and Structure of Metallothioneins Phytochelatins and Phytochelatin–Metal Complexes Structural Properties of Metallochaperones Chemical Analysis and Detection Metallothioneins Phytochelatins Occurrence Metallothioneins Phytochelatins Metallochaperones Functions Metal Homeostasis and the Role of Metallochaperones Buffering and Detoxification Physiology Metallothionein Localization and Isoforms Localization and Compartmentation of Phytochelatin Synthesis Biochemistry Metal‐binding Characteristics of Metallothioneins Biochemistry of Phytochelatin Synthesis Molecular Genetics Metallothionein Genes and Their Regulation Phytochelatin Synthase Genes Biotechnological Applications Patents Outlook and Perspectives

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The Role of Abscisic Acid in Stress Tolerance

Cho

Schwartzenberg²,

Quatrano

2018

Annual Plant Reviews Online

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Mosses have evolved complex mechanisms to overcome abiotic stresses. The phytohormone abscisic acid (ABA) plays an important role in the establishment of stress tolerance in vascular plants as well as in bryophytes. ABA pre‐treatment greatly enhances tolerance to dehydration, cold temperature and osmotic stresses in the mossPhyscomitrella patens. The response to abiotic stresses and ABA inP. patensis accompanied by the accumulation of sugars along with morphological and physiological changes. Some of the genes regulated by ABA inP. patensare also controlled by the ABA‐signalling pathway in vascular plants. However, there are genes expressed in response to ABA and abiotic stress inP. patensthat are not found in seed plants. With the ability to easily delete and replace genes by homologous recombination, as well as a fully sequenced and assembled genome,P. patenswill provide an excellent model for studying stress biology in plants.

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Cadmium lets increase the glutathione pool in bryophytes

Cited by 62 publications

References 39 publications

Increase of Total Glutathione as a Response to Cd Induced Stress in a Chilean Endemic Bryophytes (Thuidium Sp.)

Increase of Total Glutathione as a Response to Cd Induced Stress in a Chilean Endemic Bryophytes (Thuidium Sp.)

Heavy Metal‐binding Proteins and Peptides

The Role of Abscisic Acid in Stress Tolerance

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