Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis

Wu, Qi; Su, Nana; Chen, Qin; Shen, Wenbiao; Shen, Zhenguo; Xia, Yan; Chen, Jin

doi:10.1371/journal.pone.0139956

Cited by 21 publications

(17 citation statements)

References 65 publications

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“…Researchers have found that some abiotic stresses increase endogenous H 2 production in higher plants, and H 2 could alleviate damage induced by abiotic stresses [12,13]. Wu et al reported that H 2 alleviated Cd stress through ROS detoxification by reestablishing glutathione homeostasis [22]. Chen found that HRW pretreatment remarkably attenuated heat stress-induced lipid peroxidation and antioxidant dysregulation in cucumber leaves [23].…”

Section: H 2 -Evolving and H 2 -Uptake Activities Existed In Pm Vesicmentioning

confidence: 99%

Hydrogen evolution and absorption phenomena in plasma membrane of higher plants

Zhang

et al. 2020

Preprint

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Molecular hydrogen (H 2 ) exhibits a wide breadth of botanical effects especially in improving the resistance to abiotic stress. However, the primary target is not clear. Bidirectional hydrogenases have been well studied in microbes and alga, but there is a paucity of research in higher plants. Here, we used real-time spectrophotometry, gas chromatography and H 2 sensor to detect the H 2 -evolving and H 2 -uptake activity of the plasma membrane (PM) vesicles of higher plants. Net H + , Na + and K + fluxes were measured using Non-invasive Micro-test Technology (NMT). We found that the PM vesicles of Vigna radiata hypocotyls and Capsicum annuum stems have the ability of both oxidizing and producing H 2 . This two-way enzymatic activity coupled with the redox of ferricyanide/ferrocyanide and NADH/NAD + . H 2 affected the H + and Na + fluxes on the surface of V. radiata hypocotyls. These results demonstrate that the PM vesicles of higher plants have the abilities of H 2 evolution and absorption. The bidirectional activities may act as a valve to regulate the energy balance in plant cells.

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Section: H 2 -Evolving and H 2 -Uptake Activities Existed In Pm Vesicmentioning

confidence: 99%

Hydrogen evolution and absorption phenomena in plasma membrane of higher plants

Zhang

et al. 2020

Preprint

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“…Research has demonstrated that the content of GSH reduction is important in maintaining a redox environment [71]. H 2 might be an important regulatory factor in improving the tolerance of Brassica campestris seedlings against Cd, primarily by governing GSH homeostasis [72]. Dai et al [73] suggested that HRW alleviated Cd toxicity chiefly by reducing oxidative damage promoting sulfur compound metabolism and maintaining nutrient element homeostasis in alfalfa.…”

Section: Heavy Metal Stressmentioning

confidence: 99%

“…Enhancing the activities of the antioxidant enzyme [8] Cole Cd stress affected the balance of glutathione Governing reduced glutathione homeostasis [72] Alfalfa Cd stress obviously inhibited alfalfa seedling growth…”

Section: Plant Species Abiotic Stress and Its Effect H 2 Roles Under mentioning

confidence: 99%

Research Progress on the Functions of Gasotransmitters in Plant Responses to Abiotic Stresses

Yao

Yang

et al. 2019

Plants

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Abiotic stress is one of the major threats affecting plant growth and production. The harm of abiotic stresses includes the disruption of cellular redox homeostasis, reactive oxygen species (ROS) production, and oxidative stress in the plant. Plants have different mechanisms to fight stress, and these mechanisms are responsible for maintaining the required homeostasis in plants. Recently, the study of gasotransmitters in plants has attracted much attention, especially for abiotic stress. In the present review, abiotic stressors were mostly found to induce gasotransmitter production in plants. Meanwhile, these gasotransmitters can enhance the activity of several antioxidant enzymes, alleviate the harmfulness of ROS, and enhance plant tolerance under various stress conditions. In addition, we introduced the interaction of gasotransmitters in plants under abiotic stress. With their promising applications in agriculture, gasotransmitters will be adopted in the near future.Plants 2019, 8, 605 2 of 23 unique and regulate specific biological functions. Gasotransmitters have long been of great interest in a wide range of fields. Over the past few decades, the roles of these signaling molecules have been extensively studied for their biological applications. Recently, the emissions of endogenous gasotransmitters in plants have been widely studied and analyzed, thereby providing information to facilitate our understanding of new gasotransmitter signaling pathways. Previous studies found that in response to abiotic stressors, plants usually produce these gasotransmitters [12][13][14]. In addition, there is now considerable evidence to show that gasotransmitters can play a pivotal role in enhancing plant tolerance [2,15,16]. Subsequently, biological gases from complex extra and intracellular pathways and gas mediators may regulate many processes in an antagonistic or synergistic way.In the present review, we introduce the production of gasotransmitters in plants under abiotic stress. Meanwhile, we focused on the recent advances in the roles of gasotransmitters under abiotic stresses and their interaction with other gasotransmitters.

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“…In plants, H 2 was found to play roles in root formation by interacting with the nitric oxide and haem oxygenase-1/carbon monoxide pathways [8,9]. Interestingly, accumulating reports have shown that H 2 production is elevated in many plant species under abiotic stresses, such as paraquat, NaCl, high light, UV-A, UV-B, and heavy metal stresses, and H 2 can further act as a regulator in plants coping with abiotic stresses [5,[10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, sulfur and GSH metabolism are important processes in plants coping with Cd [23][24][25][26]. Recent reports have indicated that H 2 could enhance Cd tolerance by increasing antioxidant capacities, including the gene expression of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT), and by reestablishing reduced glutathione homeostasis in Medicago sativa and Brassica campestris, respectively [15,27]. However, the underlying mechanism governing glutathione metabolism in H 2 -induced Cd tolerance has not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism

et al. 2020

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Background: Hydrogen gas (H 2) is hypothesised to play a role in plants that are coping with stresses by regulating signal transduction and gene expression. Although the beneficial role of H 2 in plant tolerance to cadmium (Cd) has been investigated previously, the corresponding mechanism has not been elucidated. In this report, the transcriptomes of alfalfa seedling roots under Cd and/or hydrogen-rich water (HRW) treatment were first analysed. Then, the sulfur metabolism pathways were focused on and further investigated by pharmacological and genetic approaches. Results: A total of 1968 differentially expressed genes (DEGs) in alfalfa seedling roots under Cd and/or HRW treatment were identified by RNA-Seq. The DEGs were classified into many clusters, including glutathione (GSH) metabolism, oxidative stress, and ATP-binding cassette (ABC) transporters. The results validated by RT-qPCR showed that the levels of relevant genes involved in sulfur metabolism were enhanced by HRW under Cd treatment, especially the genes involved in (homo)glutathione metabolism. Additional experiments carried out with a glutathione synthesis inhibitor and Arabidopsis thaliana cad2-1 mutant plants suggested the prominent role of glutathione in HRW-induced Cd tolerance. These results were in accordance with the effects of HRW on the contents of (homo)glutathione and (homo)phytochelatins and in alleviating oxidative stress under Cd stress. In addition, the HRW-induced alleviation of Cd toxicity might also be caused by a decrease in available Cd in seedling roots, achieved through ABC transportermediated secretion. Conclusions: Taken together, the results of our study indicate that H 2 regulated the expression of genes relevant to sulfur and glutathione metabolism and enhanced glutathione metabolism which resulted in Cd tolerance by activating antioxidation and Cd chelation. These results may help to elucidate the mechanism governing H 2-induced Cd tolerance in alfalfa.

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Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis

Cited by 21 publications

References 65 publications

Hydrogen evolution and absorption phenomena in plasma membrane of higher plants

Hydrogen evolution and absorption phenomena in plasma membrane of higher plants

Research Progress on the Functions of Gasotransmitters in Plant Responses to Abiotic Stresses

Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism

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