Cadmium-induced changes of gypsy moth larval mass and protease activity

Vlahović, Milena; Ilijin, Larisa; Lazarević, Jelica; Mrdaković, Marija; Gavrilović, Anja; Matić, Dragana; Perić-Mataruga, Vesna

doi:10.1016/j.cbpc.2013.11.002

Cited by 13 publications

(3 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is interesting, that in our experiment, after intense stress, recovery of enzyme activity was not detected. This could be a consequence of decreased food intake, which can lead to a mass decrease in gypsy moth larvae as we discovered earlier (Vlahović et al 2009(Vlahović et al , 2014. During cadmium stress, some compensatory mechanisms probably maintain homeostasis by activating specific detoxification mechanisms.…”

Section: Enzyme Inhibitionmentioning

confidence: 81%

“…Based on the Cd content in Cd(NO 3 )·4H 2 O, the diet was supplemented with 10 or 30 μg Cd/g dry food-no observed effect concentration (NOEC) and the lowest observed effect concentration (LOEC) for relative growth rate, respectively. As previously described (Vlahović et al 2009(Vlahović et al , 2012(Vlahović et al , 2013(Vlahović et al , 2014, seven experimental groups were formed: control individuals (Cont) were fed on the basic artificial diet without cadmium contamination. For the acute treatments (Ac 1 and Ac 2 ), larvae were given food with added cadmium for 3 days of the fourth instar (10 and 30 μg Cd/g dry food, respectively).…”

Section: Experimental Treatmentsmentioning

confidence: 99%

“…Cadmium has many toxic effects in the insect gut such as alterations in gut morphology and changes in activities of digestive enzymes and provokes induction of oxidative stress and genotoxicity (Seidman et al 1986;Augustyniak et al 2009;Yousef et al 2010;Vlahović et al 2012Vlahović et al , 2013Vlahović et al , 2014. Digestive enzymes can be used as biomarkers in environmental monitoring because they can indicate the status of organisms and their adaptability in different environments.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Alteration of the Activities of Trypsin and Leucine Aminopeptidase in Gypsy Moth Caterpillars Exposed to Dietary Cadmium

Vlahović

Ilijin

Mrdaković

et al. 2015

Water Air Soil Pollut

Self Cite

View full text Add to dashboard Cite

This paper examined the gut digestive enzymes, trypsin and leucine aminopeptidase (LAP), in gypsy moth (Lymantria dispar, L.) larvae exposed to cadmium. We analyzed the 3-day acute effect, chronic effects from hatching until sacrifice, and recovery from long-term dietary treatment with cadmium concentrations of 10 and 30 μg Cd/g dry food. The activities of both examined enzymes declined at the higher level of cadmium after both acute and chronic treatments and did not recover within 3 days of feeding a diet with no added cadmium. Leucine aminopeptidase was more sensitive because its activity was inhibited after both short-term treatments. Three trypsin and one leucine aminopeptidase isoform were detected by electrophoresis. Egg hatches (full-sib families) differed in enzyme activities, index of phenotypic plasticity, and isozyme expression after different treatments. Statistically significant positive correlations between these enzymes pointed to common genetic regulation. Moreover, variances were higher for the control group than for cadmium treatment groups implying that these proteolytic enzymes did not participate directly in detoxification. These results suggest that, with additional research to discover the mechanisms of enzyme inhibition, trypsin and leucine aminopeptidase might be used as biomarkers to indicate the severity of gastrointestinal disease due to cadmium intake.

show abstract

Section: Enzyme Inhibitionmentioning

confidence: 81%