Cadmium-free quantum dots as time-gated bioimaging probes in highly-autofluorescent human breast cancer cells

Mandal, Gopa; Darragh, Molly R.; Wang, Y. Andrew; Heyes, Colin D.

doi:10.1039/c2cc37529j

Cited by 87 publications

(64 citation statements)

References 39 publications

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“…Specifically, in the time-resolved mode, the long lifetime can distinguish NC fluorescence signals from the fast decaying autofluorescence in cells/tissue, and ensure NCs for more sensitive imaging. 35 …”

Section: Resultsmentioning

confidence: 99%

Preparation of photoluminescence tunable Cu-doped AgInS₂ and AgInS₂/ZnS nanocrystals and their application as cellular imaging probes

Chen

Demillo

et al. 2016

RSC Adv.

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In this work, high-quality Cu doped AIS and AIS/ZnS NCs have been first synthesized via a surface doping approach. By varying Cu concentrations in doping, Cu doped AIS NCs exhibit a photoluminescence red-shift from around 600 nm to 660 nm with a decrease of quantum yield from around 30% to 20%. After ZnS coating or zinc etching on the Cu doped AIS NCs, Cu doped AIS/ZnS NCs present photoluminescence peaks from around 570 nm to 610 nm and high quantum yields in the range of 50 ~ 60%. Moreover, it is found that Cu doping can prolong the photoluminescence lifetime of NCs, and the average photoluminescence lifetime of Cu doped AIS and AIS/ZnS NCs is in the range of 300 ~ 500 ns. The resultant Cu doped AIS/ZnS NCs were further encapsulated with amphiphilic polymers and used as biocompatible photoluminescent probes in cellular imaging. The cellular imaging study shows that peptide-conjugated probes can specifically target U-87 brain tumor cells and thus they can be applied to the detection of endogenous targets expressed on brain tumor cells.

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Section: Resultsmentioning

confidence: 99%

Preparation of photoluminescence tunable Cu-doped AgInS₂ and AgInS₂/ZnS nanocrystals and their application as cellular imaging probes

Chen

Demillo

et al. 2016

RSC Adv.

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“…[1][2][3][4][5][6][7][8][9][10][11] On the other hand, Qdots are preferred over traditional organic dyes in cellular and in vivo imaging due to their unique optical properties, especially photo-stability, large Stokes-shifted emission and sizetuneable luminescence. [1][2][3][4][5][6][7][8][9][10][11][12][13] Thus a combination of the two i.e. SPION-Qdot composite would provide the best of both options.…”

mentioning

confidence: 98%

“…However, concerns regarding cyto-toxicity of (especially heavy metal based) Qdots, their dispersibility in aqueous medium, low luminescence quantum yield (QY), ease of functionalization for targeted delivery and stability in blood and circulation life time (even for the composite) have limited their application potential. [1][2][3][4][5][6][7][8][9][10][11][12][13] For example, the use of Fe 3 O 4 -ZnS SPION-Qdot composite has been restricted due to low luminescence QY, which makes optical imaging difficult in the presence of cellular auto-fluorescence. [14][15] Surface functionalization of the nanocrystals with appropriate ligand or compound may hold the key to colloidal and circulation stability, targeted delivery and even improvement in QY through passivation or other means.…”

mentioning

confidence: 99%

Surface Complexation-Based Biocompatible Magnetofluorescent Nanoprobe for Targeted Cellular Imaging

Bhandari

Khandelia

Pan

et al. 2015

ACS Appl. Mater. Interfaces

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We report the synthesis of a magnetofluorescent biocompatible nanoprobe-following room temperature complexation reaction between Fe3O4-ZnS nanocomposite and 8-hydroxyquinoline (HQ). The composite nanoprobe exhibited high luminescence quantum yield, low rate of photobleaching, reasonable excited-state lifetime, luminescence stability especially in human blood serum, superparamagnetism and no apparent cytotoxicity. Moreover, the nanoprobe could be used for spatio-controlled cell labeling in the presence of an external magnetic field. The ease of synthesis and cell labeling in vitro make it a suitable candidate for targeted bioimaging applications.

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“…Specifically, it is worth mentioning that the surprisingly long photoluminescence lifetime of AIS/ZnS NCs prepared with InCl 3 precursor is attractive for these NCs to be used superior bioimaging probes. The long photoluminescence lifetimes can distinguish the probe signal from the fast decaying autofluorescence in biological systems [34], and thus these NCs can be used as a tool for ultrasensitive biomedical diagnostics. Future work will focus on applications of all prepared NCs as well as the effects of chloride surface passivation on optical properties of AIS NCs.…”

Section: Resultsmentioning

confidence: 99%

Heat-up synthesis of Ag–In–S and Ag–In–S/ZnS nanocrystals: Effect of indium precursors on their optical properties

Chen

Ahmadiantehrani

Zhao

et al. 2016

Journal of Alloys and Compounds

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Cadmium-free I-III-VI nanocrystals (NCs) have recently attracted much research interests due to their excellent optical properties and low toxicity. In this work, with a simple heat-up synthetic system to prepare high quality Ag-In-S (AIS) NCs and their core/shell structures (AIS/ZnS NCs), we investigated the effect of different indium precursors (indium acetate and indium chloride) on NC optical properties. The measurements on photoluminescence spectra of AIS NCs show that the photoluminescence peak-wavelength of AIS NCs using indium acetate is in the range from 596 to 604 nm, and that of AIS NCs using indium chloride is from 641 to 660 nm. AIS and AIS/ZnS NCs using indium acetate present around 15% and 40% QYs, and both AIS and AIS/ZnS NCs using indium chloride present around 31% QYs. The photoluminescence decay study indicates that the lifetime parameters of AIS and AIS/ZnS using indium chloride are 2 ~ 4 times larger than those of AIS and AIS/ZnS NCs using indium acetate. Moreover, AIS NCs using indium chloride have a slower photobleaching dynamics than AIS NCs using indium acetate, and ZnS shell coating on both types of AIS NCs significantly enhances their photostability against UV exposure. We believe that the unique optical properties of AIS and AIS/ZnS NCs will open an avenue for these materials to be employed in broad electronic or biomedical applications.

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Cadmium-free quantum dots as time-gated bioimaging probes in highly-autofluorescent human breast cancer cells

Cited by 87 publications

References 39 publications

Preparation of photoluminescence tunable Cu-doped AgInS₂ and AgInS₂/ZnS nanocrystals and their application as cellular imaging probes

Preparation of photoluminescence tunable Cu-doped AgInS₂ and AgInS₂/ZnS nanocrystals and their application as cellular imaging probes

Surface Complexation-Based Biocompatible Magnetofluorescent Nanoprobe for Targeted Cellular Imaging

Heat-up synthesis of Ag–In–S and Ag–In–S/ZnS nanocrystals: Effect of indium precursors on their optical properties

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Cadmium-free quantum dots as time-gated bioimaging probes in highly-autofluorescent human breast cancer cells

Cited by 87 publications

References 39 publications

Preparation of photoluminescence tunable Cu-doped AgInS2 and AgInS2/ZnS nanocrystals and their application as cellular imaging probes

Preparation of photoluminescence tunable Cu-doped AgInS2 and AgInS2/ZnS nanocrystals and their application as cellular imaging probes

Surface Complexation-Based Biocompatible Magnetofluorescent Nanoprobe for Targeted Cellular Imaging

Heat-up synthesis of Ag–In–S and Ag–In–S/ZnS nanocrystals: Effect of indium precursors on their optical properties

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Preparation of photoluminescence tunable Cu-doped AgInS₂ and AgInS₂/ZnS nanocrystals and their application as cellular imaging probes

Preparation of photoluminescence tunable Cu-doped AgInS₂ and AgInS₂/ZnS nanocrystals and their application as cellular imaging probes