The genus Opuntia (Cactaceae) includes different plants well adapted to arid and semi-arid zones. These species are cultivated under restricted growth conditions, not suitable for the growth of other fruits/vegetables. The cactus pear is a well-known example inside Opuntia genus. Its young cladodes, also known as nopalitos, are frequently consumed and used in folk medicine due to their beneficial effects and phytochemical composition. Herein, hydrophilic and lipophilic extracts from cladodes of Opuntia microdasys and Opuntia macrorhiza were Opuntia species regarding a determined application of its natural extracts/isolated compounds.