CaAlaAT1 catalyzes the alanine: 2-oxoglutarate aminotransferase reaction during the resistance response against Tobacco mosaic virus in hot pepper

Kim, Ki-Jeong; Park, Chang Jin; An, Jong Min; Ham, Byung Kook; Lee, Boo Ja; Paek, Kyung Hee

doi:10.1007/s00425-005-1500-1

Cited by 22 publications

(13 citation statements)

References 40 publications

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“…3; Table 1). In parallel with our observation, Kim et al . (2005) reported in hot pepper that AlaAT was inducible by viral and bacterial pathogens, senescence, SA and ethylene and followed a similar induction pattern to PR‐1, linking this protein to defence against pathogens.…”

Section: Resultssupporting

confidence: 93%

Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus)

Hukkanen

Kokko

Buchala

et al. 2008

Molecular Plant Pathology

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SUMMARYBenzothiadiazole (BTH) induces resistance to the downy mildew pathogen, Peronospora sparsa, in arctic bramble, but the basis for the BTH-induced resistance is unknown. Arctic bramble cv. Mespi was treated with BTH to study the changes in leaf proteome and to identify proteins with a putative role in disease resistance. First, BTH induced strong expression of one PR-1 protein isoform, which was also induced by salicylic acid (SA). The PR-1 was responsive to BTH and exogenous SA despite a high endogenous SA content (20-25 μg/g fresh weight), which increased to an even higher level after treatment with BTH. Secondly, a total of 792 protein spots were detected in two-dimensional gel electrophoresis, eight proteins being detected solely in the BTHtreated plants. BTH caused up-or down-regulation of 72 and 31 proteins, respectively, of which 18 were tentatively identified by mass spectrometry. The up-regulation of flavanone-3-hydroxylase, alanine aminotransferase, 1-aminocyclopropane-1-carboxylate oxidase, PR-1 and PR-10 proteins may partly explain the BTHinduced resistance against P. sparsa. Other proteins with changes in intensity appear to be involved in, for example, energy metabolism and protein processing. The decline in ATP synthase, triosephosphate isomerase, fructose bisphosphate aldolase and glutamine synthetase suggests that BTH causes significant changes in primary metabolism, which provides one possible explanation for the decreased vegetative growth of foliage and rhizome observed in BTH-treated plants.

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Section: Resultssupporting

confidence: 93%

Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus)

Hukkanen

Kokko

Buchala

et al. 2008

Molecular Plant Pathology

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“…Furthermore, we observed an up-regulation of HSR203J that was previously shown to be transcriptionally up-regulated during the HR-PCD (Takahashi et al, 2004). In addition, we observed an up-regulation of alanine aminotransferase (AlaAT), which was shown to be transcriptionally up-regulated in Capsicum annuum undergoing a TMV-P 0 - or Xanthomonas campestris -induced HR-PCD (Kim et al, 2005). Our data suggests that the transcriptional up-regulation of HSR203J and AlaAT also results in an up-regulation of their protein abundance, and that it occurs early on during HR-PCD.…”

Section: Resultsmentioning

confidence: 94%

Induced ER Chaperones Regulate a Receptor-like Kinase to Mediate Antiviral Innate Immune Response in Plants

Caplan

Zhu

Mamillapalli

et al. 2009

Cell Host & Microbe

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Summary The plant innate immune response requires a rapid, global reprogramming of cellular processes. Here we employed two complementary proteomic methods, two-dimensional differential in-gel electrophoresis (2D-DIGE) and iTRAQ, to identify differentially regulated proteins early during a defense response. Besides defense-related proteins, the constituents of the largest category of up-regulated proteins were cytoplasmic- and endoplasmic reticulum (ER)-residing molecular chaperones. Silencing of ER-resident protein disulfide isomerases, NbERp57 and NbP5, and the calreticulins, NbCRT2 and NbCRT3, lead to a partial loss of N immune receptor-mediated defense against Tobacco mosaic virus (TMV). Furthermore, NbCRT2 and NbCRT3 are required for the expression of a novel induced receptor-like kinase (IRK). IRK is a plasma membrane-localized protein required for the N-mediated hypersensitive response programmed cell death (HR-PCD) and resistance to TMV. These data support a model in which ER-resident chaperones are required for the accumulation of membrane bound or secreted proteins that are necessary for innate immunity.

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“…To clarify whether the phenomenon of in vitro abscission is associated with senescence we monitored the expression of AlaAT1 , a maker gene of senescence in Capsicum (Kim et al. , 2005).…”

Section: Resultsmentioning

confidence: 99%

Involvement of hydrogen peroxide in leaf abscission signaling, revealed by analysis with an in vitro abscission system in Capsicum plants

et al. 2008

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SummaryAlthough auxin and ethylene play pivotal roles in leaf abscission, the subsequent signaling molecules are poorly understood. This is mainly because it is difficult to effectively treat the intact abscission zone (AZ) with pharmacological reagents. We developed an in vitro experimental system that reproduces stress-induced leaf abscission in planta. In this system, 1-mm-thick petiole strips, encompassing the AZ, were separated within 4 days of abscission at the AZ through cell wall degradation in an auxin depletion-and ethylene-dependent manner. The system allowed us to show that hydrogen peroxide (H 2 O 2 ) is involved in abscission signaling. Microscopic analyses revealed continuous H 2 O 2 production by AZ cells. H 2 O 2 scavengers and diphenylene iodonium, an inhibitor of NADPH oxidase, suppressed in vitro abscission and cellulase expression. Conversely, the application of H 2 O 2 promoted in vitro abscission and expression of cellulase. Ethephon-induced abscission was suppressed by inhibitors of H 2 O 2 production, whereas the expression of ethylene-responsive genes was unaffected by both H 2 O 2 and an H 2 O 2 inhibitor. These results indicated that H 2 O 2 acts downstream from ethylene in in vitro abscission signaling. In planta, salinity stress induced the expression of genes that respond to ethylene and reactive oxygen species, and also induced H 2 O 2 production at the AZ, which preceded leaf abscission. These results indicate that H 2 O 2 has roles in leaf abscission associated with ethylene both in vitro and in planta.

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CaAlaAT1 catalyzes the alanine: 2-oxoglutarate aminotransferase reaction during the resistance response against Tobacco mosaic virus in hot pepper

Cited by 22 publications

References 40 publications

Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus)

Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus)

Induced ER Chaperones Regulate a Receptor-like Kinase to Mediate Antiviral Innate Immune Response in Plants

Involvement of hydrogen peroxide in leaf abscission signaling, revealed by analysis with an in vitro abscission system in Capsicum plants

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