Ca2+ regulation in the absence of the iplA gene product in Dictyostelium discoideum

Schaloske, Ralph; Lusche, Daniel F.; Bezares-Roder, Karen; Happle, Kathrin; Malchow, Dieter; Schlatterer, Christina

doi:10.1186/1471-2121-6-13

Cited by 28 publications

(7 citation statements)

References 32 publications

(56 reference statements)

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“…69,70 Finally, knockout of iplA (inositol 1,4,5-trisphosphate receptor-like protein) delays aggregation by 1-2 hours. 71 The observations that grlJ ¡ and iplA ¡ cells, which are linked to calcium homeostasis, display similar developmental timing as cln3 ¡ cells was interesting given that altered calcium homeostasis has been observed in mammalian cell models that lack functional CLN3. 14,17,22 Calcium is an important signaling molecule throughout Dictyostelium development, and is required for a number of processes including migration, adhesion, and aggregation.…”

Section: Discussionmentioning

confidence: 99%

Aberrant adhesion impacts early development in aDictyosteliummodel for juvenile neuronal ceroid lipofuscinosis

Huber

Myre

Cotman

2016

Cell Adhesion & Migration

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Neuronal ceroid lipofuscinosis (NCL), also known as Batten disease, refers to a group of severe neurodegenerative disorders that primarily affect children. The most common subtype of the disease is caused by loss-of-function mutations in CLN3, which is conserved across model species from yeast to human. The precise function of the CLN3 protein is not known, which has made targeted therapy development challenging. In the social amoeba Dictyostelium discoideum, loss of Cln3 causes aberrant mid-to-late stage multicellular development. In this study, we show that Cln3-deficiency causes aberrant adhesion and aggregation during the early stages of Dictyostelium development. cln3 cells form ∼30% more multicellular aggregates that are comparatively smaller than those formed by wild-type cells. Loss of Cln3 delays aggregation, but has no significant effect on cell speed or cAMP-mediated chemotaxis. The aberrant aggregation of cln3 cells cannot be corrected by manually pulsing cells with cAMP. Moreover, there are no significant differences between wild-type and cln3 cells in the expression of genes linked to cAMP chemotaxis (e.g., adenylyl cyclase, acaA; the cAMP receptor, carA; cAMP phosphodiesterase, pdsA; g-protein α 9 subunit, gpaI). However, during this time in development, cln3 cells show reduced cell-substrate and cell-cell adhesion, which correlate with changes in the levels of the cell adhesion proteins CadA and CsaA. Specifically, loss of Cln3 decreases the intracellular level of CsaA and increases the amount of soluble CadA in conditioned media. Together, these results suggest that the aberrant aggregation of cln3 cells is due to reduced adhesion during the early stages of development. Revealing the molecular basis underlying this phenotype may provide fresh new insight into CLN3 function.

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Section: Discussionmentioning

confidence: 99%

Aberrant adhesion impacts early development in aDictyosteliummodel for juvenile neuronal ceroid lipofuscinosis

Huber

Myre

Cotman

2016

Cell Adhesion & Migration

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“…Deletion of the homolog of the IP3 receptor (IplA) alone, which abolishes chemoattractant-triggered Ca 2+ increase without perturbing resting Ca 2+ concentration in the cytosol, does not affect any of the chemotactic responses [166]. However, iplA-null cells might still have very low Ca 2+ influx that complicates the conclusion of Ca 2+ independence [167]. Furthermore, Ca 2+ chelation reduces cell spreading and lowers speed during chemotaxis, and extracellular Ca 2+ can improve cAMP-mediated migration at certain concentrations [168-171].…”

Section: Chemotactic Network Of Dictyostelium and Leukocytesmentioning

confidence: 99%

Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes

Artemenko

Lampert

Devreotes

2014

Cell. Mol. Life Sci.

178

221

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Chemotaxis, or directed migration of cells along a chemical gradient, is a highly coordinated process that involves gradient sensing, motility, and polarity. Most of our understanding of chemotaxis comes from studies of cells undergoing amoeboid-type migration, in particular the social amoeba Dictyostelium discoideum and leukocytes. In these amoeboid cells the molecular events leading to directed migration can be conceptually divided into four interacting networks: receptor/G protein, signal transduction, cytoskeleton, and polarity. The signal transduction network occupies a central position in this scheme as it receives direct input from the receptor/G protein network, as well as feedback from the cytoskeletal and polarity networks. Multiple overlapping modules within the signal transduction network transmit the signals to the actin cytoskeleton network leading to biased pseudopod protrusion in the direction of the gradient. The overall architecture of the networks, as well as the individual signaling modules are remarkably conserved between Dictyostelium and mammalian leukocytes, and the similarities and differences between the two systems are the subject of this review.

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“…It is conceivable that at 100 μM, EGTA was unable to chelate sufficient extracellular Ca 2+ to impact on cytosol free Ca 2+ . In Dictyostelium discoideum , Schaloske et al (2005) observed that 1 mM EGTA treatment was unable to produce perceptible change in the cytosol free Ca 2+ concentration, however, 10 mM was enough to reduce the intracellular concentration significantly. Clapham (2007) argues that in low Ca 2+ medium, plasma membrane Ca 2+ ATPases pump Ca 2+ out of the cell, to deplete the intracellular stores.…”

Section: Discussionmentioning

confidence: 98%

Subversion of Phytomyxae Cell Communication With Surrounding Environment to Control Soilborne Diseases; A Case Study of Cytosolic Ca2+ Signal Disruption in Zoospores of Spongospora subterranea

et al. 2022

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Ca2+ signaling regulates physiological processes including chemotaxis in eukaryotes and prokaryotes. Its inhibition has formed the basis for control of human disease but remains largely unexplored for plant disease. This study investigated the role of Ca2+ signaling on motility and chemotaxis of Spongospora subterranea zoospores, responsible for root infections leading to potato root and tuber disease. Cytosolic Ca2+ flux inhibition with Ca2+ antagonists were found to alter zoospore swimming patterns and constrain zoospore chemotaxis, root attachment and zoosporangia infection. LaCl3 and GdCl3, both Ca2+ channel blockers, at concentrations ≥ 50 μM showed complete inhibition of zoospore chemotaxis, root attachment and zoosporangia root infection. The Ca2+ chelator EGTA, showed efficient chemotaxis inhibition but had relatively less effect on root attachment. Conversely the calmodulin antagonist trifluoperazine had lesser effect on zoospore chemotaxis but showed strong inhibition of zoospore root attachment. Amiloride hydrochloride had a significant inhibitory effect on chemotaxis, root attachment, and zoosporangia root infection with dose rates ≥ 150 μM. As expected, zoospore attachment was directly associated with root infection and zoosporangia development. These results highlight the fundamental role of Ca2+ signaling in zoospore chemotaxis and disease establishment. Their efficient interruption may provide durable and practical control of Phytomyxea soilborne diseases in the field.

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Ca2+ regulation in the absence of the iplA gene product in Dictyostelium discoideum

Cited by 28 publications

References 32 publications

Aberrant adhesion impacts early development in aDictyosteliummodel for juvenile neuronal ceroid lipofuscinosis

Aberrant adhesion impacts early development in aDictyosteliummodel for juvenile neuronal ceroid lipofuscinosis

Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes

Subversion of Phytomyxae Cell Communication With Surrounding Environment to Control Soilborne Diseases; A Case Study of Cytosolic Ca2+ Signal Disruption in Zoospores of Spongospora subterranea

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