Ca2+ oscillations induced by histamine H1 receptor stimulation in HeLa cells: Fura-2 and patch clamp analysis

Regulation of PLCβ1a membrane anchoring by its substrate phosphatidylinositol (4,5)-bisphosphate

“…Two models have been described to explain oscillations similar to the ones observed for Ca 2+ , PtdIns(4,5)P 2 and PLCβ1a in this paper. The first model is based on calcium-induced calcium release, whereas the other is triggered by dynamic phosphorylation of the GPCR, thereby uncoupling the PLCβ pathway (Hirose et al, 1999;Nash et al, 2001;Sauve et al, 1991). Both models depend on PKC activity.…”

Reactive Blue 2 Induces Calcium Oscillations in HeLa Cells.

“…We also found that RB-induced Ca 2ϩ oscillations had unique characteristics different from the other receptor-activated Ca 2ϩ oscillations reported in HeLa cells [6][7][8].…”

“…In fact, RB enhanced Ca 2ϩ responses to histamine. Histamine induces a Ca 2ϩ response through the activation of the H 1 -receptor, G protein, PLC, and IP 3 receptor [6][7][8]. Our data suggest that RB acts upstream of PLC, i.e., on receptors, G protein, or PLC.…”

“…Histamine is known to induce a Ca 2ϩ response followed by Ca 2ϩ oscillations through the activation of G-protein, PLC, and IP 3 -induced Ca 2ϩ release [6][7][8]. So we used histamine as a tool to activate intracellular pathways.…”

“…Histamine induces a Ca 2ϩ response followed by Ca 2ϩ oscillations in HeLa cells via the activation of G-protein, PLC (phospholipase C) cascade, and IP 3 receptors on the endoplasmic reticulum (ER) [6][7][8]. Histamine-induced Ca 2ϩ oscillations are primarily caused by Ca 2ϩ release from the ER, but they also require Ca 2ϩ entry to maintain or modulate Ca 2ϩ oscillations [7,8].…”