Reactive Blue 2 Induces Calcium Oscillations in HeLa Cells.

Abstract

Calcium ion is an intracellular second messenger important for cellular functions in a variety of cells. In many cell types, transient and repetitive increases in the intracellularHere we found that the application of RB induced Ca 2ϩ oscillations in HeLa cells. We also found that RB-induced Ca 2ϩ oscillations had unique characteristics different from the other receptor-activated Ca 2ϩ oscillations reported in HeLa cells [6][7][8]. Materials and MethodsHeLa cells were grown in DME/F12 (SIGMA, MO, USA) supplemented with 10% fetal bovine serum, 50 units/ml penicillin, and 50 g/ml streptomycin. Cells were cultured on glass coverslips (25-mm diameter, MATSUNAMI #1) in a humidified atmosphere (5% CO 2 , 95% air) at 37°C.To introduce fura-2 into the cells, they were incubated with 2 M fura-2 AM (acetoxymethyl ester; Dojindo, Kumamoto, Japan) and 0.2% cremophor EL (SIGMA) for 50 min at 37°C. Coverslips bearing the

Triazine dyes are agonists of the NAADP receptor

“…When microsomes were challenged with either NAADP (2 μ M ) or RR120 (100 μ M ), a similar amount of Ca 2+ release was evoked. It has been previously shown that RR120 and Reactive Blue 2 are capable of activating the ryanodine receptor ( Xu et al ., 1989 ) and that Reactive Blue 2 is capable of inducing Ca 2+ oscillations in HeLa cells ( Okuda et al ., 2001 ). Furthermore, CB and Patent blue have been shown to compete for IP 3 binding in rat liver ( Bootman et al ., 1990 ) Therefore, further analysis was required to prove whether NAADP and RR120 released Ca 2+ via the same mechanism.…”

ATP‐induced calcium oscillations and change of P2Y subtypes with culture conditions in HeLa cells

“…[5][6][7][8] In another case, Reactive Blue 2 induced Ca 2þ oscillations in both a PLC and extracellular Ca 2þ dependent manner. 9 ATP is now recognized as an important extracellular messenger in many types of cells and tissues, especially non-excitable cells. For example, ATP signalling is involved in, the control of neuro-circuit activities by astrocytes in the brain, 10,11 ciliary beating in airway epithelial cells, [12][13][14] wound repair 15 and morphological change 16 in endothelial cells in blood vessels, bile secretion and the increase of metabolic activities in the liver, 17,18 activation of platelets, 19 bone formation by osteoblasts, 20 pain reception in the kidney 21,22 and sensory systems, 23 and cell-to-cell interaction and milk secretion in mammary epithelial cells.…”