Ca-Alginate-Carob Galactomannans Beads to Preserve Viability During Digestive Hostility Transit and Cholesterol Uptake Ability of Probiotic Bacteria

Ziar, Hasnia; Yahla, Imène; Riazi, Ali; Gérard, Philippe

doi:10.1007/978-3-030-43268-3_22

Cited by 1 publication

(1 citation statement)

References 16 publications

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“…The method of Ziar et al [22] was followed. In brief, MRS-THIO medium containing 2% (w/v) sodium thioglycolate was supplemented with 85 µg/mL of water-soluble cholesterol, previously sterilized by filtration on a membrane Millipore (C1145, cholesterol-PEG 600; Sigma).…”

Section: Cholesterol Uptakementioning

confidence: 99%

Probiotic Bacteria from Human Milk Can Alleviate Oral Bovine Casein Sensitization in Juvenile Wistar Rats

et al. 2023

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This study aims to see if probiotic bacteria from human milk could ameliorate oral cow’s milk sensitization. The probiotic potential of the SL42 strain isolated from the milk of a healthy young mother was first determined. Rats were then randomly gavaged with cow’s milk casein without an adjuvant or assigned to the control group. Each group was further subdivided into three groups, with each receiving only Limosilactobacillus reuteri DSM 17938, SL42, or a phosphate-buffered saline solution. Body weight, temperature, eosinophils, serum milk casein-specific IgE (CAS-IgE), histamine, and serum S100A8/A9 and inflammatory cytokine concentrations were measured. The animals were sacrificed after 59 days; histological sections were prepared, and the spleen or thymus weights, as well as the diversity of the gut microbiota, were measured. On days 1 and 59, SL42 abridged systemic allergic responses to casein by dropping histamine levels (25.7%), CAS-specific IgE levels (53.6%), eosinophil numbers (17%), S100A8/9 (18.7%), and cytokine concentrations (25.4–48.5%). Analyses of histological sections of the jejunum confirmed the protective effect of probiotic bacteria in the CAS-challenged groups. Lactic acid bacteria and Clostridia species were also increased in all probiotic-treated groups. These findings suggest that probiotics derived from human milk could be used to alleviate cow’s milk casein allergy.

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Section: Cholesterol Uptakementioning

confidence: 99%