Probiotic Bacteria from Human Milk Can Alleviate Oral Bovine Casein Sensitization in Juvenile Wistar Rats

Abstract

This study aims to see if probiotic bacteria from human milk could ameliorate oral cow’s milk sensitization. The probiotic potential of the SL42 strain isolated from the milk of a healthy young mother was first determined. Rats were then randomly gavaged with cow’s milk casein without an adjuvant or assigned to the control group. Each group was further subdivided into three groups, with each receiving only Limosilactobacillus reuteri DSM 17938, SL42, or a phosphate-buffered saline solution. Body weight, temperature, eosinophils, serum milk casein-specific IgE (CAS-IgE), histamine, and serum S100A8/A9 and inflammatory cytokine concentrations were measured. The animals were sacrificed after 59 days; histological sections were prepared, and the spleen or thymus weights, as well as the diversity of the gut microbiota, were measured. On days 1 and 59, SL42 abridged systemic allergic responses to casein by dropping histamine levels (25.7%), CAS-specific IgE levels (53.6%), eosinophil numbers (17%), S100A8/9 (18.7%), and cytokine concentrations (25.4–48.5%). Analyses of histological sections of the jejunum confirmed the protective effect of probiotic bacteria in the CAS-challenged groups. Lactic acid bacteria and Clostridia species were also increased in all probiotic-treated groups. These findings suggest that probiotics derived from human milk could be used to alleviate cow’s milk casein allergy.

Probiotic Properties of Lactic Acid Bacteria Newly Isolated from Algerian Raw Cow’s Milk

“…Raw milk (from cows, goats, buffalos, sheep, camels, and donkeys), as well as human breast milk, are sources of heterogeneous lactic flora, commonly used to isolate new LAB strains with important probiotic potential, which aroused the interest of several research teams around the world [ 1 , 25 , 26 , 27 ]. LABs have a GRAS (Generally Recognized As Safe) status and many probiotic properties, giving them a multitude of opportunities to be used in the food industry [ 28 ].…”

In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk

“…In general, human breast milk contains approximately 10 3 to 10 4 colony forming units/mL of microbes such as Lactobacilli, Lactococci, Enterococci, and Leuconostoc spp. (Keddar et al, 2023). Among bacteria present in breast milk, Lactiplantibacillus plantarum strains have been identified as potential probiotics (Mollova et al, 2023).…”

Special Issue “Probiotics, Prebiotics and Functional Foods: Health Benefits and Biosafety”

“…Keddar et al [ 6 ] demonstrated that probiotic bacteria from human milk, specifically the SL42 strain, have the potential to reduce the allergic response to cow’s milk casein in rats. The SL42 strain decreased histamine levels, milk casein-specific IgE levels, eosinophil numbers, S100A8/9 levels, and cytokine concentrations, while also increasing LAB and Clostridium species in the gut microbiota.…”