C60 fullerenes increase the intensity of rotational movements in non-anesthetized hemiparkinsonic rats

Abstract

The effect of C60 fullerene aqueous colloid solution (C60FAS) on the intensity of long-lasting (persisting for one hour) rotational movements in non-anesthetized rats was investigated. For this purpose, an experimental hemiparkinsonic animal model was used in the study. Rotational movements in hemiparkinsonic animals were initiated by the intraperitoneal administration of the dopamine receptor agonist apomorphine. It was shown that a preliminary injection of C60FAS (a substance with powerful antioxidant properties) in hemiparkinsonic rats induced distinct changes in animal motor behavior. It was revealed that fullerene-pretreated animals, in comparison with non-pretreated or vehicle-pretreated rats, rotated for 1 h at an approximately identical speed until the end of the experiment, whereas the rotation speed of control rats gradually decreased to 20-30% of the initial value. One can assume that the observed changes in the movement dynamics of the hemiparkinsonic rats after C60FAS pretreatment presumably can be induced by the influence of C60FAS on the dopaminergic system, although the isolated potentiation of the action of apomorphine C60FAS cannot be excluded. Nevertheless, earlier data on the action of C60FAS on muscle dynamics has suggested that C60FAS can activate a protective action of the antioxidant system in response to long-lasting muscular activity and that the antioxidant system in turn may directly decrease fatigue-related changes during long-lasting muscular activity.

C60 Fullerene Reduces 3-Nitropropionic Acid-Induced Oxidative Stress Disorders and Mitochondrial Dysfunction in Rats by Modulation of p53, Bcl-2 and Nrf2 Targeted Proteins

“…In the present study, 3-NPA application in a high dose caused body weight decrease and movement abnormalities in rats, which were improved significantly by C 60 treatment in both pre-and post-treatment regimens. A resemblance to the beneficial effect of C 60 on skeletal muscle functions and structure was noted in our recent studies where we have shown a significant decrease in skeletal muscle fatigue during prolonged and intense physical loads [24,25] and an increase in the movement dynamics of the hemiparkinsonian rats after C 60 injection [48].…”

Fatigue-induced Fos immunoreactivity within the lumbar cord and amygdala decreases after С60 fullerene pretreatment

“…Well-known that the enhancement of free radical processes is the main pathogenic factor in the development of skeletal muscle fatigue 31 and that intensive physical activity induces overproduction of free radicals in muscle tissue 32 . At the same time, the use of exogenous antioxidants can lead to a significant decrease in skeletal muscle fatigue during prolonged and/or intense loads [33][34][35][36] . It was previously reported that the application of the C 60 fullerenes can intensify anti-inflammatory effects 37 and regulate the ATPase activity of actomyosin 38 .…”

Analysis of Biomechanical Parameters of Muscle Soleus Contraction and Blood Biochemical Parameters in Rat with Chronic Glyphosate Intoxication and Therapeutic Use of C60 Fullerene

“…It should be noted that the use of selected doses of C 60 FAS are based on previous experimentally established data, which showed a high protective effect of water-soluble C 60 fullerenes [ 13 , 14 , 19 ]. Additionally, it should be noted that the doses of C 60 fullerene used in our experiments are significantly lower than the LD 50 value, which was 600 mg/kg body weight when administered orally to rats [ 40 ] and 721 mg/kg when administered intraperitoneally to mice [ 25 ].…”

“…It is assumed that biocompatible and water-soluble C 60 fullerenes [ 12 ] can be considered as powerful scavengers of free radicals during the development of ischemia and fatigue processes in skeletal muscle [ 13 , 14 ]. In our previous works on in vivo models, it was shown that the usage of safe doses of water-soluble C 60 fullerene at the initiation of various pathologies leads to significant positive therapeutic effects, in particular, during acute liver injury, colorectal cancer, obesity, acute cholangitis and hemiparkinsonism [ 15 , 16 , 17 , 18 , 19 , 20 ].…”