C50 carotenoids extracted from Haloterrigena thermotolerans strain K15: antioxidant potential and identification

Kesbic, Fevziye Isil; Gültepe, Nejdet

doi:10.1007/s12223-021-00905-w

Cited by 16 publications

(16 citation statements)

References 33 publications

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“…The temperature and pH values of brine samples were measured in the sampling area by a portable digital multiparameter meter (WTW, Multi 3430) and the salinity was measured by a benchtop multiparameter meter (WTW, Multi 9420) in the laboratory. The molten agar medium JCM 168 (casamino acids; 5 g/L, L-glutamic acid; 1 g/L, yeast extract; 5 g/L, trisodium citrate; 3 g/L, MgSO 4 .7H 2 O; 29.5 g/L, KCl; 2 g/L, NaCl; 175.5 g/L, FeCl 2 .4H 2 O; 0.036 g/L, MnCl 2 .4H 2 O; 0.36 mg/L) was used for culturing the strains [2,17]. The colonies with pink-red pigmentation were selected for the study.…”

Section: Identification Of Haloarchaeal Strainsmentioning

confidence: 99%

“…Since high temperature and high salinity reduce the amount of oxygen, they show very intense growth performance at 45-50°C when adequate ventilation is provided [1]. In addition to all these features, halophilic archaea have unique C 50 carotenoid pigments, and these molecules are the pigments that give the colonies their typical red color as well as showing protective properties in natural conditions [2,3].…”

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confidence: 99%

“…Radical scavenging activity measured by DPPH is a useful tool to understand the antioxidant activity of carotenoids in studies [2,3]. The conjugated double bond system provides carotenoid molecules with a sturdy and linear skeleton structure, as well as a high reduction potential, thereby making them potential antioxidants.…”

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confidence: 99%

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Carotenoid characterization, fatty acid profiles, and antioxidant activities of haloarchaeal extracts

Kesbiç,

Gültepe

2023

J Basic Microbiol

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Microorganisms that can survive in saline environments, known as halotolerant or halophilic organisms, have a wide range of current and potential uses in biotechnology. In this study, it was aimed to determine the carotenoids of halophilic archaea strains isolated from the brine samples taken from different points of Salt Lake (Turkey) and determine the antioxidant activities of their carotenoids. To identify the halophilic archaea strains, they were cultivated in MAM JCM 168 medium and subjected to antibiotic susceptibility, fatty acid, two‐dimensional and three‐dimensional imaging by scanning electron microscopy and atomic force microscopy, biochemical and phylogenetic assays. The findings show that five different halophilic archaea strains have been identified as Halorubrum lipolyticum, Halorubrum sodomense, Haloarcula salaria, Halorubrum chaoviator, and Haloarcula japonica with 98% and above similarity ratio. The main fatty acids of all haloarchaeal strains were octadecanoic acid (C18:0) and palmitic acid (C16:0). The major carotenoid of the species was determined as all‐trans bacterioruberin, and different carotenoid types such as lycopene, β‐carotene, and 2‐isopentenyl‐3,4‐dehydrorodopin were found as well as bacterioruberin isomers. The antioxidant activities of carotenoids extracted from the species were analyzed by the 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging method and the extracts showed antioxidant activity statistically significantly higher than ascorbic acid and butylated hydroxytoluene as reference products (p < 0.05).

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Section: Identification Of Haloarchaeal Strainsmentioning

confidence: 99%

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confidence: 99%

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Carotenoid characterization, fatty acid profiles, and antioxidant activities of haloarchaeal extracts

Kesbiç,

Gültepe

2023

J Basic Microbiol

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“…Regarding the purication step, some works used thin-layer chromatography. 17,[27][28][29][30][31][32] However, this technique, despite being simple and efficient at the lab scale, is not industrially adequate. In this sense, more efforts are needed to develop efficient and sustainable approaches with scale-up potential encompassing the valorization of multiple products obtained from this biomass.…”

Section: Introductionmentioning

confidence: 99%

Recovery of bacterioruberin and proteins using aqueous solutions of surface-active compounds

et al. 2022

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“…Carotenoids in general, particularly those from marine origins, are excellent antioxidants 38 – 40 . This is attributed to structural properties such as the conjugated double-bond length and the existing chemical groups 41 – 44 . In our study, two of the identified pigments were C 50 -carotenoids, characterized by 13 conjugated double bonds and the presence of a hydroxyl group 45 .…”

Section: Discussionmentioning

confidence: 99%

Optimization and multiple in vitro activity potentials of carotenoids from marine Kocuria sp. RAM1

Metwally

El‐Sersy

El-Sikaily

et al. 2022

Sci Rep

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Marine pigmented bacteria are a promising natural source of carotenoids. Kocuria sp. RAM1 was isolated from the Red Sea Bohadschia graeffei collected from Marsa Alam, Egypt, and used for carotenoids production. The extracted carotenoids were purified by thin-layer chromatography (TLC). The characteristic UV absorbance of the three purified fractions gave us an inkling of what the purified pigments were. The chemical structures were confirmed by nuclear magnetic resonance spectroscopy (NMR) and LC-ESI-QTOF-MS/MS. The three different red pigments were identified as two C50-carotenoids, namely bisanhydrobacterioruberin and trisanhydrobacterioruberin, in addition to 3,4,3ʹ,4ʹ-Tetrahydrospirilloxanthin (C42-carotenoids). Kocuria sp. RAM1 carotenoids were investigated for multiple activities, including antimicrobial, anti-inflammatory, antioxidant, anti-HSV-1, anticancer, antidiabetic and wound healing. These new observations suggest that Kocuria sp. RAM1 carotenoids can be used as a distinctive natural pigment with potent properties.

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C50 carotenoids extracted from Haloterrigena thermotolerans strain K15: antioxidant potential and identification

Cited by 16 publications

References 33 publications

Carotenoid characterization, fatty acid profiles, and antioxidant activities of haloarchaeal extracts

Carotenoid characterization, fatty acid profiles, and antioxidant activities of haloarchaeal extracts

Recovery of bacterioruberin and proteins using aqueous solutions of surface-active compounds

Optimization and multiple in vitro activity potentials of carotenoids from marine Kocuria sp. RAM1

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