Optimization and multiple in vitro activity potentials of carotenoids from marine Kocuria sp. RAM1

Abstract

Marine pigmented bacteria are a promising natural source of carotenoids. Kocuria sp. RAM1 was isolated from the Red Sea Bohadschia graeffei collected from Marsa Alam, Egypt, and used for carotenoids production. The extracted carotenoids were purified by thin-layer chromatography (TLC). The characteristic UV absorbance of the three purified fractions gave us an inkling of what the purified pigments were. The chemical structures were confirmed by nuclear magnetic resonance spectroscopy (NMR) and LC-ESI-QTOF-MS/MS. The three different red pigments were identified as two C50-carotenoids, namely bisanhydrobacterioruberin and trisanhydrobacterioruberin, in addition to 3,4,3ʹ,4ʹ-Tetrahydrospirilloxanthin (C42-carotenoids). Kocuria sp. RAM1 carotenoids were investigated for multiple activities, including antimicrobial, anti-inflammatory, antioxidant, anti-HSV-1, anticancer, antidiabetic and wound healing. These new observations suggest that Kocuria sp. RAM1 carotenoids can be used as a distinctive natural pigment with potent properties.

Microbial communities on UV cabins in the dermatology service of a Spanish hospital: ecology and resistance to UV light and antibiotics

“…6B and 6C). Some Kocuria strains have been reported as highly resistant to different types of radiation, as well as to synthesize carotenoids and encode genes related to oxidative stress [41,44,[72][73][74]. Moreover, Deinococcus spp.…”

Biosynthesis, characterization and optimization of TiO2 nanoparticles by novel marine halophilic Halomonas sp. RAM2: application of natural dye-sensitized solar cells

“…250 ml of methanol were added to the pellet, which was then incubated in a water bath at 40 °C for 15 min until the dye recovered completely. The extract was purified, dissolved in petroleum ether, and stored in a refrigerator away from direct light [ 30 ].…”

“…Kocuria sp. RAM1 dye is a mixture of three carotenoid compounds, namely bisanhydrobacterioruberin, trisanhydrobacterioruberin (C 50 -carotenoids) and 3,4,3',4'-tetrahydrospirilloxanthin (C 42 -carotenoids) [ 30 ].…”

Computational Insight into Intraspecies Distinctions in Pseudoalteromonas distincta: Carotenoid-like Synthesis Traits and Genomic Heterogeneity

“…In general, marine pigmented bacteria are known to be a promising source of melanins and carotenoids as natural high antioxidant efficiency compounds with rare structures, as well as a plethora of other highly specific secondary metabolites with biotechnology and therapy applications [ 8 , 9 , 10 , 11 , 12 ]. Carotenoids are isoprenoid derivatives, characterized by a polyene chain (C30, C40, or C50), with conjugated double bonds providing distinctive absorption patterns and coloration.…”

“…Melanins are also distributed as oxygen radical scavengers and virulence factors among microorganisms and are formed by the oxidative polymerization of phenolic or indolic compounds [ 8 , 26 ]. A lot of marine bacterial pigments were investigated for antimicrobial, anti-inflammatory, antioxidant, anti-HSV-1, anticancer, antidiabetic, and wound healing activities, indicating their potential biomedical applications as well as food, feed, cosmetic, and pharmaceutical formulations [ 8 , 9 , 12 ]. In addition, the natural marine pigments and other secondary metabolites could be used as “green chemistry” to replace synthetic compounds [ 11 , 12 , 26 , 27 , 28 , 29 , 30 ].…”