C18 core-shell column with in-series absorbance and fluorescence detection for simultaneous monitoring of changes in stilbenoid and proanthocyanidin concentrations during grape cane storage

Sáez, Vania; Gayoso, Camila; Riquelme, Sebastián A.; Pérez, Julie; Vergara, Carola; Mardones, Claudia; Baer, Dietrich von

doi:10.1016/j.jchromb.2017.12.028

Cited by 22 publications

(37 citation statements)

References 24 publications

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“…For all samples, (+)‐catechin concentrations were higher than those of (−)‐epicatechin (between 74% and 50% of total flavonols content), whereas the sample MB3 showed an opposing tendency. These flavanols have also been reported in previous studies, with maximum values found for (+)‐catechin and (−)‐epicatechin of 1100 and 500 μg g −1 DW, respectively . It is interesting to note that the concentrations found for (+)‐catechin and (−)‐epicatechin in the present study are similar to those reported for grape pomace extracts (GPE) .…”

Section: Resultssupporting

confidence: 90%

Bioactive compounds and total antioxidant capacity of cane residues from different grape varieties

et al. 2019

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BACKGROUND: Every year, the viticulture activity generates considerable amounts of underused lignocellulosic residues as grape cane, which are generally composted or burned despite their potential value as a source of bioactive compounds. Determination of their phytochemical composition and total antioxidant capacity (TAC) may be a useful way of exploiting different high-added value applications. RESULTS: Twenty-one phenolic compounds (PC) and two carotenoids (Car) were quantified by high performance-liquid chromatography-diode array detection in eight grape varieties from different locations inMendoza, Argentina. The maximum concentrations corresponded to the stilbene -viniferin [10 552 g g −1 dry weight (DW)], followed by the flavanols (+)-catechin (3718 g g −1 DW) and (−)-epicatechin (2486 g g −1 DW). In addition, lutein and -carotene were quantified at levels ranging between 350 and 2400 ng g −1 DW. The TAC of the extracts was assessed by oxygen radical absorbance capacity , 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid and 1,1-diphenyl-2-picrylhydrazyl assays, with a good correlation between TAC and total PC for each sample (r ≥ 0.82). CONCLUSION: Samples of cv. Malbec, the most representative variety of Argentina's winemaking industry, presented high contents of PC, particularly -viniferin, (+)-catechin and (−)-epicatechin. Quercetin-3-galactoside, OH-tyrosol and Car were reported for the first time in grape canes of the eight varieties. The results add to the existing knowledge related to this inexpensive source of high-value bioactive compounds, which could be used as functional ingredients.

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Section: Resultssupporting

confidence: 90%

Bioactive compounds and total antioxidant capacity of cane residues from different grape varieties

et al. 2019

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“…It has already been demonstrated that, after cutting, grape canes if stored properly over some months, accumulate large quantities of stilbenoid . This increase depends on storage time, temperature, relative moisture during storage, grape variety, size of cane pieces during storage, and whether the grapes were cultivated using a conventional or organic mode . However, the combined effect of pruning and storage time on the stilbene accumulation rate has not been studied before.…”

Section: Resultsmentioning

confidence: 99%

Influence of pruning time and viral infection on stilbenoid levels in Pinot noir grape canes

et al. 2020

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BACKGROUND: Grapevine canes represent a large source of waste derived from grape cultivation. In the present study, the effect of different processes of storage and different pruning times on the stilbene accumulation on Pinot noir canes was analyzed. Whether the alteration of the secondary metabolism accompanying leafroll symptom expressions could affect the stilbenoid accumulation in canes harvested at pruning time was also investigated.RESULTS: The maximum accumulation of trans-resveratrol and trans-piceatannol was obtained in canes harvested in October and dried at 40°C. Even in grape canes harvested in October, November, and December and stored for different times at room temperature (20 ± 2°C) a marked increase in trans-resveratrol and trans-piceatannol was evident, which reached a maximum at around 8 weeks of storage. A significant higher accumulation of trans-resveratrol and trans-piceatannol was also found in canes harvested from symptomatic plants compared to those harvested from asymptomatic plants for all the pruning times.CONCLUSION: This study confirms that the biosynthetic enzyme activities and, particularly, those involved in the stilbene pathway, persist during Pinot noir cane storage at different harvest times, with different storage times and conditions, and different sanitary status.

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“…The Gorena et al [ 76 ] method (method 2, Table 2 ) uses 2 g of the sample extracted with 16 milliliters of ethanol:H 2 O (80:20 v / v ), with an ultrasonic homogenizer at 50 Hz for 5 min. This methodology was also applied by Sáez et al [ 82 ] to calculate the stilbene concentration in Pinot noir grapevine canes. It was detected that methods 1 and 2 only differed in the amount of solvent and the kind of ultrasonic technology employed; however, with method 1 only t -resveratrol and t -ε-viniferin were quantified, whereas with method 2 other stilbenes such as ampelopsin A, piceatannol, hopeaphenol, and r-viniferin were also detected ( Table 2 ).…”

Section: Extraction Methodsmentioning

confidence: 99%

“…Sáez et al [ 82 ] (Analysis 8, Table 3 ) proposed the use of an HPLC system coupled in series to a DAD, an FLD, and a triple-quadrupole mass spectrometer for the identification of stilbenes in grapevine canes. Detection using DAD was performed at 306 and 280 nm, and for FLD the excitation and emission wavelengths were 330 and 374 nm for the stilbenoids.…”

Section: Grapevine Cane Extract Analysismentioning

confidence: 99%

Grapevine Cane Extracts: Raw Plant Material, Extraction Methods, Quantification, and Applications

2020

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Grapevine canes are viticulture waste that is usually discarded without any further use. However, recent studies have shown that they contain significant concentrations of health-promoting compounds, such as stilbenes, secondary metabolites of plants produced as a response to biotic and abiotic stress from fungal disease or dryness. Stilbenes have been associated with antioxidant, anti-inflammatory, and anti-microbial properties and they have been tested as potential treatments of cardiovascular and neurological diseases, and even cancer, with promising results. Stilbenes have been described in the different genus of the Vitaceae family, the Vitis genera being one of the most widely studied due to its important applications and economic impact around the world. This review presents an in-depth study of the composition and concentration of stilbenes in grapevine canes. The results show that the concentration of stilbenes in grapevine canes is highly influenced by the Vitis genus and cultivar aspects (growing conditions, ultraviolet radiation, fungal attack, etc.). Different methods for extracting stilbenes from grapevine canes have been reviewed, and the extraction conditions have also been studied, underlining the advantages and disadvantages of each technique. After the stilbenes were extracted, they were analyzed to determine the stilbene composition and concentration. Analytical techniques have been employed with this aim, in most cases using liquid chromatography, coupled with others such as mass spectrometry and/or nuclear magnetic resonance to achieve the individual quantification. Finally, stilbene extracts may be applied in multiple fields based on their properties. The five most relevant are preservative, antifungal, insecticide, and biostimulant applications. The current state-of-the-art of the above applications and their prospects are discussed.

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C18 core-shell column with in-series absorbance and fluorescence detection for simultaneous monitoring of changes in stilbenoid and proanthocyanidin concentrations during grape cane storage

Cited by 22 publications

References 24 publications

Bioactive compounds and total antioxidant capacity of cane residues from different grape varieties

Bioactive compounds and total antioxidant capacity of cane residues from different grape varieties

Influence of pruning time and viral infection on stilbenoid levels in Pinot noir grape canes

Grapevine Cane Extracts: Raw Plant Material, Extraction Methods, Quantification, and Applications

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