C-type lectin 5, a novel pattern recognition receptor for the JAK/STAT signaling pathway in Bombyx mori

Geng, Tao; Lu, Fuping; Wu, Hua-zhou; Wang, Yongsong; Lou, D; Tu, Na-Na; Zhu, Feng; Wang, Shuchang

doi:10.1016/j.jip.2020.107473

Cited by 15 publications

(11 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is described that this pathway can neutralize DENV and West Nile Virus (WNV) infection ( Avirutnan et al., 2011 ; Fuchs et al., 2011 ). Moreover, it was recently described that CTLs can act as a recognition receptor for JAK-STAT immune pathway ( Geng et al., 2021 ), which downregulation may interfere in AMPs production, as gambicin peptide. Those downregulations combined can facilitate ZIKV infection.…”

Section: Resultsmentioning

confidence: 99%

Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia

Ramos

Martins²,

Murillo³

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Zika is a vector-borne disease caused by an arbovirus (ZIKV) and overwhelmingly transmitted by Ae. aegypti. This disease is linked to adverse fetal outcomes, mostly microcephaly in newborns, and other clinical aspects such as acute febrile illness and neurologic complications, for example, Guillain-Barré syndrome. One of the most promising strategies to mitigate arbovirus transmission involves releasing Ae. aegypti mosquitoes carrying the maternally inherited endosymbiont bacteria Wolbachia pipientis. The presence of Wolbachia is associated with a reduced susceptibility to arboviruses and a fitness cost in mosquito life-history traits such as fecundity and fertility. However, the mechanisms by which Wolbachia influences metabolic pathways leading to differences in egg production remains poorly known. To investigate the impact of coinfections on the reproductive tract of the mosquito, we applied an isobaric labeling-based quantitative proteomic strategy to investigate the influence of Wolbachia wMel and ZIKV infection in Ae. aegypti ovaries. To the best of our knowledge, this is the most complete proteome of Ae. aegypti ovaries reported so far, with a total of 3913 proteins identified, were also able to quantify 1044 Wolbachia proteins in complex sample tissue of Ae. aegypti ovary. Furthermore, from a total of 480 mosquito proteins modulated in our study, we discuss proteins and pathways altered in Ae. aegypti during ZIKV infections, Wolbachia infections, coinfection Wolbachia/ZIKV, and compared with no infection, focusing on immune and reproductive aspects of Ae. aegypti. The modified aspects mainly were related to the immune priming enhancement by Wolbachia presence and the modulation of the Juvenile Hormone pathway caused by both microorganism’s infection.

show abstract

Section: Resultsmentioning

confidence: 99%

Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia

Ramos

Martins²,

Murillo³

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…It has been reported that CTLs could be involved in antifungal responses (23). Some CTLs have been reported to be directly or indirectly involved in the activation of the immune signaling pathways (24,25). LvCTL1 possesses anti-white spot syndrome virus activity by binding to virus proteins in Litopenaeus vannamei (26).…”

Section: Introductionmentioning

confidence: 99%

Hepatopancreas-Specific Lectin Participates in the Antibacterial Immune Response by Regulating the Expression of Antibacterial Proteins

Cao

Sun

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

The hepatopancreas is an important digestive and immune organ in crustacean. There were low but stable numbers of microbes living in the hemolymph of crustacean, whereas the organs (including hepatopancreas) of crustacean were immersed in the hemolymph. It is very important to study the immune mechanism of the hepatopancreas against bacteria. In this study, a novel CTL (HepCL) with two CRDs, which was mainly expressed in the hepatopancreas, was identified in red swamp crayfish (Procambarus clarkii). HepCL binds to bacteria in vitro and could enhance bacterial clearance in vivo. Compared with the C-terminal CRD of HepCL (HepCL-C), the N-terminal CRD (HepCL-N) showed weaker bacterial binding ability in vitro and stronger bacterial clearance activity in vivo. The expression of some antimicrobial proteins, such as FLP, ALF1 and ALF5, was downregulated under knockdown of HepCL or blocked with Anti-HepCL after challenge with Vibrio in crayfish. These results demonstrated that HepCL might be involved in the antibacterial immune response by regulating the expression of antimicrobial proteins.

show abstract

“…Among the arthropods, CTLs specifically recognize the PAMPs on the surface of microbes, and then trigger the aforementioned immune responses to eliminate the invading pathogens. For instance, B. mori CTL5 may be involved in the JAK/STAT pathway and slightly stimulated by B. mori cytoplasmic polyhedrosis virus (BmCPV) [ 19 ]. In mosquitos, mosGCTL-7 interacts with the envelope protein of the Japanese encephalitis virus (JEV) via N-glycan at N154, and then promotes virus entry into cells [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Bombyx mori C-Type Lectin (BmIML-2) Inhibits the Proliferation of B. mori Nucleopolyhedrovirus (BmNPV) through Involvement in Apoptosis

Mei

Peng

et al. 2022

IJMS

View full text Add to dashboard Cite

C-type lectins (CTLs) are widely distributed in mammals, insects, and plants, which act as pattern recognition receptors (PRRs) to recognize pathogens and initiate immune responses. In this study, we identified a C-type lectin gene called BmIML-2 from the silkworm Bombyx mori. Its open reading frame (ORF) encodes 314 amino acids, which contain dual tandem C-type lectin-like domain (CTLD). BmIML-2 is highly expressed in the fat body and is significantly induced at 24 h after BmNPV infection. Moreover, overexpression of BmIML-2 dramatically inhibited the proliferation of BmNPV, and knockdown assay via siRNA further validated the inhibition of BmIML-2 on viral proliferation. In addition, transcript level detection of apoptosis-related genes and observation of apoptosis bodies implied that overexpression of BmIML-2 promoted BmNPV-induced apoptosis. Immunofluorescence analysis indicated that BmIML-2 distributed throughout the cytoplasm and was slightly concentrated in the cell membrane. Taken together, our results suggest that BmIML-2 could inhibit in the proliferation of BmNPV by facilitating cell apoptosis.

show abstract

C-type lectin 5, a novel pattern recognition receptor for the JAK/STAT signaling pathway in Bombyx mori

Cited by 15 publications

References 45 publications

Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia

Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia

Hepatopancreas-Specific Lectin Participates in the Antibacterial Immune Response by Regulating the Expression of Antibacterial Proteins

Bombyx mori C-Type Lectin (BmIML-2) Inhibits the Proliferation of B. mori Nucleopolyhedrovirus (BmNPV) through Involvement in Apoptosis

Contact Info

Product

Resources

About