Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia

Ramos, Luís Felipe Costa; Martins, Michele; Murillo, Jimmy Rodriguez; Domont, Gilberto B.; Oliveira, Danielle Maria Perpétua de; Nogueira, Fábio César Sousa; Maciel-de-Freitas, Rafael; Junqueira, Magno

doi:10.3389/fcimb.2022.900608

Cited by 4 publications

(5 citation statements)

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“…Wolbachia interactions with the juvenile hormone biosynthetic pathway have been previously reported [94]. Particularly, a proteomic assay on transfected A. aegypti ovaries showed downregulation of farnesol dehydrogenase and mevalonate kinase, two enzymes in the JHIII biosynthetic pathway [96]. However, to the best of our knowledge, our study is the first showing Wolbachia ’s consistent upregulation of the putative FAMeT codifying AAEL004667 in A. aegypti .…”

Section: Resultssupporting

confidence: 54%

“…aegypti ovaries showed downregulation of farnesol dehydrogenase and mevalonate kinase, two enzymes in the JHIII biosynthetic pathway [96] . However, to the best of our knowledge, our study is the first showing Wolbachia 's consistent upregulation of the putative FAMeT codifying AAEL004667 in A. aegypti .…”

Section: Liu Et Al (2014)mentioning

confidence: 99%

“…For instance, the GO term mRNA metabolic process had a negative Normalized Enrichment Score in 10 out of 12 tests, 7 of which were significant (p<0.05) (S4 Table ). RNA viruses such as DENV, ZIKV and CHIKV have small genomes and heavily depend on host factors for the success of their life cycle, including various host RNA-interacting genes [80,95,96]. Under this consideration, downregulation of RNA-interacting genes by Wolbachia may underlie the broad-range of RNA viruses to which Wolbachia offers protection.…”

Section: Wolbachia Consistently Downregulates Genes With Nucleic Acid...mentioning

confidence: 99%

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UnveilingWolbachia pipientistranscriptomic signature inAedes aegypti: a comprehensive meta-analysis

Mejías,

Jiménez,

Conca

et al. 2023

Preprint

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BackgroundAn effective strategy for arboviral control consists in transfectingAedes aegyptimosquitoes with the intracellular bacteriaWolbachia pipientis, which reduces host viral susceptibility and spreads itself into wild populations via reproductive manipulations. However, the prospect of losing the efficacy of this strategy underscores the need for deepening the mechanistic knowledge ofDiptera-Wolbachiasystems and identifying relevantWolbachiaeffects that could decline upon adaptation ofA. aegyptitransfections. A systematic comparison of publicly availableDiptera-Wolbachiatranscriptomic datasets could yield progress in this matter.Methodology/Principal findingsWe derived differentially expressed gene (DEG) sets from previously publishedDiptera-Wolbachiatranscriptomic datasets, subjected them to enrichment analysis of Gene Ontology terms, and intersected the results to identify patterns of host gene/function regulation byWolbachia. A putative farnesoic acid methyl transferase (AAEL004667) and a flavin-containing monooxygenase (AAEL000834) were consistently upregulated in transfectedA. aegyptiand linked to cytoplasmic incompatibility and viral susceptibility, being proposed as novel targets of study. Genes implicated in viral blocking—GNBPA1,PGRPS1,DEFC,Tf1, serine-type endopeptidases and endopeptidase inhibitors— were consistently upregulated in transfectedA. aegyptibut not in native infections, indicating that they could lose responsiveness toWolbachiaover time and should be considered to keep the efficacy of arboviral control. The commonality of chitinase regulation byWolbachiawas identified and proposed as an explanation for the loss of desiccation resistance in transfectedA. aegypti’s eggs, which is a main obstacle for the introgression ofWolbachiain mosquito populations.Conclusions/SignificanceThe present work points out relevant gene targets to consider for arboviral control sustainability and provides new hypotheses for deepening the understanding ofDiptera-Wolbachiasystems.Author SummaryArboviral diseases (e.g. dengue), which are mainly transmitted by the mosquitoAedes aegypti, impose a global public health crisis. An effective strategy for controlling the spread of these diseases is to artificially infectA. aegyptipopulations with the bacteriaWolbachia pipientis, which reduces its capacity to transmit arboviruses. However, future adaptive changes in the novelA. aegypti-Wolbachiaassociation could diminish the efficacy of this approach. To prevent this, it is crucial to have a solid biological understanding ofWolbachiainfections and predictions about specific changes that artificial infections could undergo. By analyzing publicly available biological data fromWolbachia-infected mosquitoes and flies we were able to propose new hypotheses regarding general aspects ofWolbachiainfection and to identify antiviral effects ofWolbachiainA. aegyptithat could decline over time, thus providing relevant information for keeping sustainability of a key arboviral control strategy.

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Section: Resultssupporting

confidence: 54%

Section: Liu Et Al (2014)mentioning

confidence: 99%

Section: Wolbachia Consistently Downregulates Genes With Nucleic Acid...mentioning

confidence: 99%

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UnveilingWolbachia pipientistranscriptomic signature inAedes aegypti: a comprehensive meta-analysis

Mejías,

Jiménez,

Conca

et al. 2023

Preprint

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“…Aedes aegypti females when infected with DENV or ZIKV have a reduction in life-history traits such as survivorship, fecundity, and fertility [ 61 , 62 , 63 , 64 , 65 ]. On a molecular and biochemical level, there are abundant data using gene expression, transcriptomics, and proteomics approaches revealing that most of the altered genes are involved in metabolic processes, cellular processes, and proteolysis [ 58 , 66 , 67 , 68 ]. Thus, most likely the NIR detects those physiological changes caused by infection and then is able to differentiate between infected and uninfected individuals.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Non-Invasive Detection of Aedes aegypti Co-Infected with Zika and Dengue Viruses Using Near Infrared Spectroscopy

Garcia

Lord

Santos

et al. 2022

Viruses

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The transmission of dengue (DENV) and Zika (ZIKV) has been continuously increasing worldwide. An efficient arbovirus surveillance system is critical to designing early-warning systems to increase preparedness of future outbreaks in endemic countries. The Near Infrared Spectroscopy (NIRS) is a promising high throughput technique to detect arbovirus infection in Ae. aegypti with remarkable advantages such as cost and time effectiveness, reagent-free, and non-invasive nature over existing molecular tools for similar purposes, enabling timely decision making through rapid detection of potential disease. Our aim was to determine whether NIRS can differentiate Ae. aegypti females infected with either ZIKV or DENV single infection, and those coinfected with ZIKV/DENV from uninfected ones. Using 200 Ae. aegypti females reared and infected in laboratory conditions, the training model differentiated mosquitoes into the four treatments with 100% accuracy. DENV-, ZIKV-, and ZIKV/DENV-coinfected mosquitoes that were used to validate the model could be correctly classified into their actual infection group with a predictive accuracy of 100%, 84%, and 80%, respectively. When compared with mosquitoes from the uninfected group, the three infected groups were predicted as belonging to the infected group with 100%, 97%, and 100% accuracy for DENV-infected, ZIKV-infected, and the co-infected group, respectively. Preliminary lab-based results are encouraging and indicate that NIRS should be tested in field settings to evaluate its potential role to monitor natural infection in field-caught mosquitoes.

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“…ZIKV transmission can be vector-borne or non-vector-borne 1 . Vector-borne transmission occurs when the virus infects the host through mosquito bites 36 . This explains the sylvatic transmission cycle, where non-human primates and endemic mosquitoes (enzootic vectors) are involved, as well as human-mosquito-human transmission in urban and suburban regions 1,37 .…”

Section: Zikvmentioning

confidence: 99%

Congenital Zika syndrome

Crisanto-López¹,

Jesús²,

López-Quecho³

et al. 2023

BMHIM

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In February 2016, the World Health Organization declared Zika virus (ZIKV) infection a public health emergency of international concern because it caused congenital Zika syndrome (CZS). The CZS is considered a specific pattern of birth defects caused by ZIKV infection, which is transmitted by the bite of the Aedes aegypti mosquito. The CZS clinical manifestations are broad and nonspecific, including microcephaly, subcortical calcifications, ocular alterations, congenital contractures, early hypertonia, and pyramidal as well as extrapyramidal symptoms. The ZIKV has gained great importance because it has affected a large percentage of the population worldwide during the last few years, despite the measures implemented by international organizations. The pathophysiology and non-vectorial transmission routes of the virus are still under study. The diagnosis is made upon suspicion of ZIKV infection, the patient's clinical manifestations, and it is confirmed by molecular laboratory tests demonstrating the presence of viral particles. Unfortunately, there is no specific treatment or vaccine for this condition; however, patients receive multidisciplinary care and constant monitoring. Therefore, the strategies that have been implemented are directed toward preventive measures and vector control.

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Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia

Cited by 4 publications

References 122 publications

UnveilingWolbachia pipientistranscriptomic signature inAedes aegypti: a comprehensive meta-analysis

UnveilingWolbachia pipientistranscriptomic signature inAedes aegypti: a comprehensive meta-analysis

Rapid and Non-Invasive Detection of Aedes aegypti Co-Infected with Zika and Dengue Viruses Using Near Infrared Spectroscopy

Congenital Zika syndrome

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