C-phycocyanin extraction from Spirulina platensis wet biomass

Moraes, Caroline Costa; Sala, Luisa; Cerveira, G. P.; Kalil, Susana Juliano

doi:10.1590/s0104-66322011000100006

Cited by 152 publications

(90 citation statements)

References 23 publications

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“…In this context, photosynthetic microorganisms such as cyanobacteria are of particular interest because they generate several compounds with diverse applications (Belay, 1997;Spolaore et al, 2006;Vonshak, 1997) in addition to food protein. A. platensis is an important source of cellular protein, essential fatty acids, phycocyanin and chlorophyll a (Belay, 1997;Spolaore et al, 2006, Moraes et al, 2011.…”

Section: Introductionmentioning

confidence: 99%

GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

Cruz-Martínez

Jesus

Matsudo

et al. 2015

Braz. J. Chem. Eng.

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-NH 4 NO 3 simultaneously provides a readily assimilable nitrogen source (ammonia) and a reserve of nitrogen (nitrate), allowing for an increase in Arthrospira platensis biomass production while reducing the cost of the cultivation medium. In this study, a 2 2 plus star central composite experimental design combined with response surface methodology was employed to analyze the influence of light intensity (I) and the total amount of added NH 4 NO 3 (M t ) on a bench-scale tubular photobioreactor for fed-batch cultures. The maximum cell concentration (X m ), cell productivity (P X ) and biomass yield on nitrogen (Y X/N ) were evaluated, as were the protein and lipid contents. Under optimized conditions (I = 148 µmol·photons·m -2 ·s -1 and M t = 9.7 mM NH 4 NO 3 ), X m = 4710 ±34.4 mg·L -1 , P X = 478.9 ±3.8 mg·L -1 ·d -1 and Y X/N = 15.87 ±0.13 mg·mg -1 were obtained. The best conditions for protein content in the biomass (63.2%) were not the same as those that maximized cell growth (I = 180 µmol·photons·m -2 ·s -1 and M t = 22.5 mM NH 4 NO 3 ). Based on these results, it is possible to conclude that ammonium nitrate is an interesting alternate nitrogen source for the cultivation of A. platensis in a fed-batch process and could be used for other photosynthetic microorganisms.

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Section: Introductionmentioning

confidence: 99%

GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

Cruz-Martínez

Jesus

Matsudo

et al. 2015

Braz. J. Chem. Eng.

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“…Because of its properties, such as high nutritional value and the presence of valuable biocompounds, like phycocyanin (Moraes et al, 2011), it is currently one of the most studied microalgae. It is usually commercially produced in open bioreactors of up to 0.5 hectares in size (Belay, 1997), using sunlight as the light source.…”

Section: Introductionmentioning

confidence: 99%

Spirulina sp. LEB-18 culture using effluent from the anaerobic digestion

Borges¹,

Rosa²,

Meza³

et al. 2013

Braz. J. Chem. Eng.

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-The carbon source is the most expensive nutrient for Spirulina production; effluents from anaerobic digestion contain this nutrient in the form of HCO 3 -. The aim of this study was to assess the growth kinetics, composition and fatty acid profile of Spirulina sp. LEB-18 grown in standard Zarrouk medium (NaHCO 3 16.8 g L -1) and in Zarrouk medium replaced with 20% (v/v) effluent with reduced concentrations of NaHCO 3 (5.3 and 2.8 g L -1 ). The use of effluent and lower concentrations of HCO 3 -was found to be an alternative to reduce the costs of Spirulina production, because there were no significant differences in growth parameters (μ max 0.324 -0.354 d -1 ; P max 0.280 -0.297 g L -1 d -1 ), in the different culture medium used. Lipids ranged between 4.9 and 5.0%; the media with effluent had higher levels of linoleic acid compared to the standard medium.

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“…These unique properties will be created premier place for it in the field of nanotechnology as drug delivery agent. [18] Cefpirome is the cephalosporin derivative active against Gram-positive and Gram-negative bacteria. However, the continuous and high rate of usage causes various adverse side effects which progressively leads to severe health complications.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Namasivayam

2017

AJP

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Background and Purpose: Nanotechnology principles are extensively utilized in the development of various drugs which exhibit the improved spectrum of activity with the less side effects. Among the various nanoparticles (NPs), protein-based nanomaterial gains recent attention because of the high rate of efficacy, delivery, and less side effects. In this study, the antibacterial antibiotics cefpirome has been formulated with biocompatible ovalbumin (OVA) NPs and the nanodrug conjugate was evaluated against improved antibacterial activity, in vitro control release and nontarget toxicity against Vero cells and blood cells. Materials and Methods: The methods for the preparation of phycocyanin functionalized OVA NPs were optimized with various parameters adapting coacervation technique. The nanodrug conjugate prepared using the optimized condition was characterized using electron microscopy studies, Fourier transform infrared spectroscopy and in vitro controlled drug release by continuous dialysis method, antibacterial activity against human pathogenic bacterial strains by well diffusion, liquid colorimetric assays, and biofilm inhibition assays. Nontarget toxicity study was conducted by determination of cytotoxicity against Vero cell line and human peripheral blood cells under laboratory condition. Results: Phycocyanin functionalized nanodrug conjugate was prepared under optimum condition characterized by electron microscopy studies reveals nanosphere is in the range of 100-200 nm. The drug loading efficiency and entrapment efficiency were found to be 85.0% and 90.5%. The in vitro drug release profile showed a steady rate of release and maximum release of 99.0% during 20 h. Antibacterial activity against human pathogenic bacteria showed improved spectrum of activity against all the tested pathogenic bacteria as dose-dependent manner. Microtiter plate and nitrocellulose membrane assay were adapted to study the biofilm inhibition of pathogenic bacteria which revealed all the tested bacterial biofilm was highly inhibited in both the tested assays as concentration-dependent manner. Effect of drug conjugate on dynamic growth curve of tested bacteria also showed the drastic reduction of growth inhibition. The antibacterial activity of nanodrug conjugate was found to be retained all the tested temperature against all the tested bacterial organism. Nontarget toxicity studies showed there was no distinct toxic effect on Vero and blood cells. Conclusion: Effective inhibition of pathogenic bacteria and best biocompatibility of nanoformulated cefpirome would suggest the possible utilization of the formulation as an antibacterial agent to fight against human pathogenic bacteria in modern medicine.

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C-phycocyanin extraction from Spirulina platensis wet biomass

Cited by 152 publications

References 23 publications

GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

Spirulina sp. LEB-18 culture using effluent from the anaerobic digestion

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