C-Myb And Leukaemogenesis

c-Myc Is Essential but Not Sufficient for c-Myb-mediated Block of Granulocytic Differentiation

“…To definitely demonstrate expression of c-Myb and c-Myc proteins from the transgenes, we compared the protein levels following incubation of 32D cells in the presence of GCSF for 10 days. It was shown previously that, in 32Dcl3 cells, the endogenous levels of c-Myb and c-Myc proteins remain relatively high until day 6 -8 of GCSF treatment followed by a downward regulation of mRNA and protein, such that they become undetectable by day 10 of GCSF treatment (9,10). Taking advantage of this observation, we analyzed the levels of c-Myb and c-Myc proteins on the 10th day of GCSF treatment in the transfected cells to determine the levels of the transgenic proteins expressed in the absence of the endogenous protein product.…”

Differential modulation of Myb family genes by Ets-2

“…In contrast, the C-terminus of B-Myb has a positive regulatory function (Oh and Reddy, 1998), since deletion of this region leads to a significant loss of the protein's transactivational activity. Additionally, use of the 32Dcl3 cell model system has shown that overexpression of these myb genes resulted in different phenotypes, that is, ectopic overexpression of cmyb blocked their terminal differentiation into granulocytes (Bies et al, 1995;Patel et al, 1996), while ectopic overexpression of either A-myb or B-myb failed to induce such a block Reddy, 1997, 1998).…”