c-Myc Is Essential but Not Sufficient for c-Myb-mediated Block of Granulocytic Differentiation

Kumar, Atul; Lee, Clement; Reddy, E. Premkumar

doi:10.1074/jbc.m300080200

Cited by 12 publications

(8 citation statements)

References 40 publications

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“…These data provide direct evidence for a functional relationship between NLK and c-Myb in breast carcinoma cells. It is already known that c-Myb regulates the levels of Bcl-2 and c-myc, which are modulators of apoptosis and cell cycle arrest, respectively [33], [34]. We examined the levels of Bcl-2 and c-myc by Western blotting and found that ectopic expression of NLK downregulated c-myc, which is a target gene of c-Myb (Fig.…”

Section: Resultsmentioning

confidence: 91%

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Nemo-Like Kinase Associated with Proliferation and Apoptosis by c-Myb Degradation in Breast Cancer

Huang

Jiang

et al. 2013

PLoS ONE

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Nemo-like kinase (NLK), a mediator of the Wnt signaling pathway, binds directly to c-Myb, leading to its phosphorylation, ubiquitination and proteasome-dependent degradation. NLK was significantly downregulated in the breast cancer tissues compared to corresponding normal tissues. NLK expression was negatively correlated with c-Myb expression. NLK suppressed proliferation, induced apoptosis and mediated c-Myb degradation in MCF-7 cells via a mechanism that seems to involve c-myc and Bcl2. These findings might provide a novel target for therapeutic intervention in patients with breast cancer.

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Section: Resultsmentioning

confidence: 91%

“…c-myc is a target of c-Myb, and activation of the c-myc gene is required for Myb-mediated transformation [33]. Bcl-2 is a direct target gene of the protooncogene c-Myb [34].…”

Section: Discussionmentioning

confidence: 99%

Nemo-Like Kinase Associated with Proliferation and Apoptosis by c-Myb Degradation in Breast Cancer

Huang

Jiang

et al. 2013

PLoS ONE

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“…The downregulated transcription factors included Meis1 , c‐Myc , ERF‐2 , and HoxA9 . Meis1 , c‐Myc , and HoxA9 have all been shown to be important at the HSC level or in HSC differentiation [32, –34].…”

Section: Resultsmentioning

confidence: 99%

Gene Expression Analysis of Hematopoietic Progenitor Cells Identifies Dlg7as a Potential Stem Cell Gene

et al. 2007

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Inducible hematopoietic stem/progenitor cell lines represent a model for studying genes involved in self-renewal and differentiation. Here, gene expression was studied in the inducible human CD34؉ acute myelogenous leukemia cell line KG1 using oligonucleotide arrays and suppression subtractive cloning. Using this approach, we identified Dlg7, the homolog of the Drosophila Dlg1 tumor suppressor gene, as downregulated at the early stages of KG1 differentiation. Similarly, Dlg7 was expressed in normal purified umbilical cord blood CD34؉CD38؊ progenitors but not in the more committed CD34؉CD38؉ population. Dlg7 expression was not detected in differentiated cells obtained from hematopoietic colonies, nor was expression detected in purified T-cells, B-cells, and monocytes. When analyzed in different types of stem cells, Dlg7 expression was detected in purified human bone marrow-derived CD133؉ progenitor cells, human mesenchymal stem cells, and mouse embryonic stem (ES) cells. Overexpression of Dlg7 in mouse ES cells increased their growth rate and reduced the number of EBs emerging upon differentiation. In addition, the EBs were significantly smaller, indicating an inhibition in differentiation. This inhibition was further supported by higher expression of Bmp4, Oct4, Rex1, and Nanog in EBs overexpressing Dlg7 and lower expression of Brachyury. Finally, the Dlg7 protein was detected in liver and colon carcinoma tumors but not in normal adjacent tissues, suggesting a role for the gene in carcinogenesis. In conclusion, our results suggest that Dlg7 has a role in stem cell survival, in maintaining stem cell properties, and in carcinogenesis. STEM

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“…The discovery of haploid transcripts in mammalian sperm dates back to almost two decades when c-MYC mRNA [52] and MHC Class I transcripts [53] were detected by RT-PCR in human sperm. Since then, a number of studies have characterized individual transcripts, as well as the global transcriptome of the sperm in normal and subfertile men [6], [8], [28], [31], [54].…”

Section: Discussionmentioning

confidence: 99%

Stallion Sperm Transcriptome Comprises Functionally Coherent Coding and Regulatory RNAs as Revealed by Microarray Analysis and RNA-seq

et al. 2013

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Mature mammalian sperm contain a complex population of RNAs some of which might regulate spermatogenesis while others probably play a role in fertilization and early development. Due to this limited knowledge, the biological functions of sperm RNAs remain enigmatic. Here we report the first characterization of the global transcriptome of the sperm of fertile stallions. The findings improved understanding of the biological significance of sperm RNAs which in turn will allow the discovery of sperm-based biomarkers for stallion fertility. The stallion sperm transcriptome was interrogated by analyzing sperm and testes RNA on a 21,000-element equine whole-genome oligoarray and by RNA-seq. Microarray analysis revealed 6,761 transcripts in the sperm, of which 165 were sperm-enriched, and 155 were differentially expressed between the sperm and testes. Next, 70 million raw reads were generated by RNA-seq of which 50% could be aligned with the horse reference genome. A total of 19,257 sequence tags were mapped to all horse chromosomes and the mitochondrial genome. The highest density of mapped transcripts was in gene-rich ECA11, 12 and 13, and the lowest in gene-poor ECA9 and X; 7 gene transcripts originated from ECAY. Structural annotation aligned sperm transcripts with 4,504 known horse and/or human genes, rRNAs and 82 miRNAs, whereas 13,354 sequence tags remained anonymous. The data were aligned with selected equine gene models to identify additional exons and splice variants. Gene Ontology annotations showed that sperm transcripts were associated with molecular processes (chemoattractant-activated signal transduction, ion transport) and cellular components (membranes and vesicles) related to known sperm functions at fertilization, while some messenger and micro RNAs might be critical for early development. The findings suggest that the rich repertoire of coding and non-coding RNAs in stallion sperm is not a random remnant from spermatogenesis in testes but a selectively retained and functionally coherent collection of RNAs.

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c-Myc Is Essential but Not Sufficient for c-Myb-mediated Block of Granulocytic Differentiation

Cited by 12 publications

References 40 publications

Nemo-Like Kinase Associated with Proliferation and Apoptosis by c-Myb Degradation in Breast Cancer

Nemo-Like Kinase Associated with Proliferation and Apoptosis by c-Myb Degradation in Breast Cancer

Gene Expression Analysis of Hematopoietic Progenitor Cells Identifies Dlg7as a Potential Stem Cell Gene

Stallion Sperm Transcriptome Comprises Functionally Coherent Coding and Regulatory RNAs as Revealed by Microarray Analysis and RNA-seq

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