c- kit -Dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract

Torihashi, Shigeko; Ward, Sean M.; Nishikawa, Shin‐Ichi; Nishi, Katsuhide; Kobayashi, Shigeru; Sanders, Kenton M.

doi:10.1007/s004410050334

Cited by 150 publications

(223 citation statements)

References 0 publications

Supporting

Mentioning

205

Contrasting

Unclassified

Order By: Relevance

“…A role for kita in ICC development is indicated by data from Sparse mutants showing a reduced frequency of spontaneous GI contractions in 7-dpf larvae. Further, the increased surface area of the GI tract in Sparse mutants is highly similar to observations of an expanded small intestine in the W/Wv mutant mouse (Maeda et al, 1992;Torihashi et al, 1995). Complete c-Kit inactivation mutants or the absence of Kit ligand is lethal for the mouse model but homozygous Sparse mutants survive to adulthood.…”

Section: Discussionsupporting

confidence: 76%

See 1 more Smart Citation

Kit‐like immunoreactivity in the zebrafish gastrointestinal tract reveals putative ICC

Rich

Leddon

Hess

et al. 2007

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 76%

“…maintain ICC in adults (Maeda et al, 1992;Torihashi et al, 1995;Beckett et al, 2006). We examined homozygous Sparse mutants spa b5 (ZDB-FISH-980202-47) for functional differences in GI motility and for differences in appearance of the GI tract to determine a role for the kita gene.…”

Section: Resultsmentioning

confidence: 99%

Kit‐like immunoreactivity in the zebrafish gastrointestinal tract reveals putative ICC

Rich

Leddon

Hess

et al. 2007

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…ICC express c-kit and depend on signaling via Kit receptors for development and maintenance of phenotype (Maeda et al, 1992;Ward et al, 1994;Torihashi et al, 1995). Kit expression has provided an important means of identifying ICC and understanding the anatomical distribution of ICC in GI muscles.…”

mentioning

confidence: 99%

Interstitial Cells of Cajal Mediate Cholinergic Neurotransmission from Enteric Motor Neurons

et al. 2000

Self Cite

View full text Add to dashboard Cite

Interstitial cells of Cajal (ICC) are interposed between enteric neurons and smooth muscle cells in gastrointestinal muscles. The role of intramuscular ICC (IC-IM) in mediating enteric excitatory neural inputs was studied using gastric fundus muscles of wild-type animals and W/W v mutant mice, which lack IC-IM. Excitatory motor neurons, labeled with antibodies to vesicular acetylcholine transporter or substance-P, were closely associated with IC-IM. Immunocytochemistry showed close contacts between enteric neurons and IC-IM. IC-IM also formed gap junctions with smooth muscle cells. Electrical field stimulation yielded fast excitatory junction potentials in the smooth muscle that were blocked by atropine. Neural responses were greatly reduced in muscles of W/W v animals. Loss of cholinergic responses in W/W v muscles seemed to be caused by the loss of close synaptic contacts between motor neurons and IC-IM, because these muscles were not less responsive to exogenous acetylcholine than were wild-type muscles. W/W v muscles also responded to excitatory nerve stimulation when the breakdown of acetylcholine was blocked by neostigmine. The density of cholinergic nerve bundles within the muscles was not significantly different in wild-type and W/W v muscles, and similar amounts of 14 [C]choline were released from preloaded wildtype and W/W v muscles in response to nerve stimulation. The impact of losing IC-IM on gastric compliance was also evaluated in intact stomachs. Pressure increased as a function of fluid volume and infusion rate in wild-type animals, but W/W v animals showed little basal tone and minimal increases in pressure with fluid infusions. These data suggest that IC-IM play a major role in receiving cholinergic excitatory inputs from the enteric nervous system in the murine fundus.

show abstract

“…The slow waves originate in the ICCs distributed in the GI tract (Dickens et al, 1999;Huizinga et al, 1995;Langton et al, 1989;Torihashi et al, 1995;Ordog et al, 1999;Ward et al, 1994). ICCs express c-kit immunoreactivity and form gap junctional connections with ICCs and with smooth muscle cells (Huizinga et al, 1997;Komuro et al, 1992;Sanders, 1996).…”

Section: Discussionmentioning

confidence: 99%

Effects of Transient Receptor Potential Channel Blockers on Pacemaker Activity in Interstitial Cells of Cajal from Mouse Small Intestine

Kim

Nam

Kim

2011

Molecules and Cells

View full text Add to dashboard Cite

The interstitial cells of Cajal (ICCs) are pacemakers in the gastrointestinal tract and transient receptor potential melastatin type 7 (TRPM7) is a candidate for pacemaker channels. The effect of the 5-lipoxygenase (5-LOX) inhibitors NDGA, AA861, MK886 and zileuton on pacemaking activity of ICCs was examined using the whole cell patch clamp technique. NDGA and AA861 decreased the amplitude of pacemaker potentials in ICC clusters, but the resting membrane potentials displayed little change, respectively. Also, perfusing NDGA and AA861 into the bath reduced both inward current and outward current in TRPM7-like current in single ICC, respectively. But, they had no effects on Ca 2+ activated Cl -currents. The 5-LOX inhibitors MK886 and zileuton were, however, ineffective in pacemaker potentials in ICC clusters and in TRPM7-like current in single ICC, respectively. A specific TRPC3 inhibitor, pyrazole compound (Pyr3), and a specific TRPM4 inhibitor, 9-phenanthrol, had no effects in pacemaker potentials in ICC clusters and in TRPM7-like current in single ICC. These results suggest that, among the tested 5-LOX inhibitors, NDGA and AA861 modulate the pacemaker activities of the ICCs, and that the TRPM7 channel can affect intestinal motility.

show abstract

c- kit -Dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract

Cited by 150 publications

References 0 publications

Kit‐like immunoreactivity in the zebrafish gastrointestinal tract reveals putative ICC

Kit‐like immunoreactivity in the zebrafish gastrointestinal tract reveals putative ICC

Interstitial Cells of Cajal Mediate Cholinergic Neurotransmission from Enteric Motor Neurons

Effects of Transient Receptor Potential Channel Blockers on Pacemaker Activity in Interstitial Cells of Cajal from Mouse Small Intestine

Contact Info

Product

Resources

About