Kit‐like immunoreactivity in the zebrafish gastrointestinal tract reveals putative ICC

Rich, Adam; Leddon, Scott A; Hess, S. L.; Gibbons, Simon J.; Miller, Steven M.; Xu, Xiaolei; Farrugai, G.

doi:10.1002/dvdy.21086

Cited by 34 publications

(32 citation statements)

References 39 publications

(62 reference statements)

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“…Since then, subsequent investigations using Kit-antibodies to detect ICC in zebrafish (Danio rerio) have been conflicting, with some studies failing to detect them (Mellgren and Johnson, 2005;Parichy et al, 1999;Uyttebroek et al, 2013), whereas others have suggested the presence of putative ICC in the myenteric region and circular muscle layer (Ball et al, 2012;Rich et al, 2007). Recently, a network of Ano1-positive cells has also been described in larval and adult zebrafish (Uyttebroek et al, 2013), which in combination with our observations strongly indicate that Ano1 is a more reliable marker for ICC than Kit in the gastrointestinal tract of fish.…”

Section: Discussion Evidence For Icc In the Gastrointestinal Tract Ofmentioning

confidence: 99%

“…Findings from some of these studies have revealed that although blockade of neural activity (using tetrodotoxin, TTX) abolishes some motility patterns, other patterns persist, indicating that they are myogenic in origin (Brijs et al, 2014;Holmberg et al, 2007). The myogenically driven component of gastrointestinal motility patterns in fish has repeatedly been suggested to involve ICC (Brijs et al, 2014;Holmberg et al, 2007;Rich et al, 2013Rich et al, , 2007; however, currently our knowledge regarding the presence, distribution and function of these cells in fish is limited.…”

Section: Introductionmentioning

confidence: 99%

“…ICC were originally described over a century ago, as small fusiform or stellate cells with prominent varicose processes that formed networks in gastrointestinal tissues (Cajal, 1911). Antibodies against Kit have been used extensively to label ICC throughout the gastrointestinal tract in a wide range of vertebrates (Christensen, 1992), but success in labelling of ICC using Kit antibodies in teleosts has been variable (Ball et al, 2012;Mellgren and Johnson, 2005;Parichy et al, 1999;Rich et al, 2007;Wallace et al, 2005). Recently, anoctamin 1 (Ano1), a Ca 2+ -activated Cl − channel found to be expressed exclusively in ICC has been used as a more specific marker for ICC (Espinosa et al, 2008;Gomez-Pinilla et al, 2009;Hwang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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The presence and role of interstitial cells of Cajal in the proximal intestine of shorthorn sculpin (Myoxocephalus scorpius)

Brijs

Hennig

Kellermann

et al. 2016

Journal of Experimental Biology

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Rhythmic contractions of the mammalian gastrointestinal tract can occur in the absence of neuronal or hormonal stimulation owing to the generation of spontaneous electrical activity by interstitial cells of Cajal (ICC) that are electrically coupled to smooth muscle cells. The myogenically driven component of gastrointestinal motility patterns in fish probably also involves ICC; however, little is known of their presence, distribution and function in any fish species. In the present study, we combined immunohistochemistry and in vivo recordings of intestinal motility to investigate the involvement of ICC in the motility of the proximal intestine in adult shorthorn sculpin (Myoxocephalus scorpius). Antibodies against anoctamin 1 (Ano1, a Ca 2+ -activated Cl − channel), revealed a dense network of multipolar, repeatedly branching cells in the myenteric region of the proximal intestine, similar in many regards to the mammalian ICC-MY network. The addition of benzbromarone, a potent blocker of Ano1, altered the motility patterns seen in vivo after neural blockade with TTX. The results indicate that ICC are integral for the generation and propagation of the majority of rhythmic contractile patterns in fish, although their frequency and amplitude can be modulated via neural activity.

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Section: Discussion Evidence For Icc In the Gastrointestinal Tract Ofmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The presence and role of interstitial cells of Cajal in the proximal intestine of shorthorn sculpin (Myoxocephalus scorpius)

Brijs

Hennig

Kellermann

et al. 2016

Journal of Experimental Biology

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“…Zebrafish were obtained and cared for following methods described previously (Rich et al, 2007). Wild-type adult zebrafish were obtained from the Zebrafish International Resource Center and were maintained according to standard guidelines in accordance with IACUC guidelines (Westerfield 2000).…”

Section: Aquaculture and Cell Linesmentioning

confidence: 99%

Expression and comparative genomics of two serum response factor genes in zebrafish

et al. 2008

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Serum response factor (SRF) is a single copy, highly conserved transcription factor that governs the expression of hundreds of genes involved with actin cytoskeletal organization, cellular growth and signaling, neuronal circuitry and muscle differentiation. Zebrafish have emerged as a facile and inexpensive vertebrate model to delineate gene expression, regulation, and function, and yet the study of SRF in this animal has been virtually unexplored. Here, we report the existence of two srf genes in zebrafish, with partially overlapping patterns of expression in 3 and 7 day old developing animals. The mammalian ortholog (srf1) encodes for a 520 amino acid protein expressed in adult vascular and visceral smooth muscle cells, cardiac and skeletal muscle, as well as neuronal cells. The second zebrafish srf gene (srf2), encoding for a presumptive protein of only 314 amino acids, is transcribed at lower levels and appears to be less widely expressed across adult tissues. Both srf genes are induced by the SRF coactivator myocardin and attenuated with a short hairpin RNA to mammalian SRF. Promoter studies with srf1 reveal conserved CArG boxes that are the targets of SRF-myocardin in embryonic zebrafish cells. These results reveal that SRF was duplicated in the zebrafish genome and that its protein expression in all three muscle cell types is highly conserved across vertebrate animals suggesting an ancient code for transcriptional regulation of genes unique to muscle cell lineages.

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“…First of all, zebrafish develops outside the utero during embryonic period and maintains transparent until most organs become mature [14][15][16]. Given that we utilize the transgenic zebrafish as live biosensors to test the side effects of ketamine, whose specific regions can be labeled by some visible proteins (GFP) [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Zebrafish as a New Platform Used in Exploration of Ketamine-Induced Neurodevelopmental Toxicity

Ruan¹

2015

J Clin Exp Pathol

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We have modified the abstract as 'Previous studies have already found out that commonly used anesthetic, ketamine, has toxic effects on neurodevelopment. Unlike most rodent models just focusing on neuronal apoptosis caused by ketamine, early stages of neuron development abnormality in zebrafish can be assessed in vivo because of the transparent embryos and larve. And also thanks to its cost-efficiency and quick reproduction, large-scale behavior analyses and gene screens can be conducted in zebrafish. Besides, the whole genome of zebrafish has already been sequenced and its gene functions are highly conserved during evolution, which makes the experiments more reliable on zebrafish model. So Zebrafish has obvious advantages in the researches of ketamine neurotoxicity over the conventional animal models (such as mice). Within this paper we illuminate how we can use this model to study ketamine neurotoxicity. In the future, along with more advanced genetic technologies joining this platform will not only make up for conventional models to deeply understand neurodevelopmental toxicity of ketamine, but also might provide the unique insight to the field of neurodevelopment and neurotoxicity impaired by other drugs

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Kit‐like immunoreactivity in the zebrafish gastrointestinal tract reveals putative ICC

Abstract: Gastrointestinal (GI) motility results from the coordinated actions of enteric neurons, interstitial cells of

Cited by 34 publications

References 39 publications

The presence and role of interstitial cells of Cajal in the proximal intestine of shorthorn sculpin (Myoxocephalus scorpius)

The presence and role of interstitial cells of Cajal in the proximal intestine of shorthorn sculpin (Myoxocephalus scorpius)

Expression and comparative genomics of two serum response factor genes in zebrafish

Zebrafish as a New Platform Used in Exploration of Ketamine-Induced Neurodevelopmental Toxicity

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