C/EBP Homology Protein (CHOP) Interacts with Activating Transcription Factor 4 (ATF4) and Negatively Regulates the Stress-dependent Induction of the Asparagine Synthetase Gene

A Bifunctional Intronic Element Regulates the Expression of the Arginine/Lysine Transporter Cat-1 via Mechanisms Involving the Purine-rich Element Binding Protein A (Purα)

“…This apparent discrepancy could be due to different times of Tg treatment (4 h compared with 18 h in our study). Consistent with our data, Su et al (26) found a significant increase in binding of CHOP to the Cat-1 gene in vivo during Tg treatment. However, the interpretation of this finding is difficult because the AARE and INE are separated by 355 bp and could be isolated on the same DNA fragment in this ChIP experiment.…”

“…A recent report by Su and Kilberg (26) showed that CHOP associates with ATF4 during stress to attenuate transcription of the ASNS gene via its AARE. The authors also concluded that the Cat-1 AARE is not a target of CHOP repression because Cat-1 mRNA levels were not affected by CHOP overexpression.…”

“…with the recent report documenting the inhibition of ASNS gene transcription by CHOP (26). This sustained induction of both Cat-1 and ASNS mRNAs during late ER stress was partially attenuated by transient overexpression of CHOP in CHOP Ϫ/Ϫ MEFs (Fig.…”

C/EBP Homologous Protein (CHOP) Contributes to Suppression of Metabolic Genes during Endoplasmic Reticulum Stress in the Liver

“…It was originally proposed that CHOP inhibited transcription by simple DNA-independent titration of these family members, and this appears to be the case in at least some instances (25,31). However, CHOP can also bind to DNA when in complex with other proteins, including both C/EBP family members and ATF family members (45,46). Although heterodimers containing CHOP can activate transcription (46,47), such heterodimers most commonly appear to attenuate induction of transcription compared with the activity of the binding partner when CHOP is absent (39,44,46).…”

Looking at the metabolic consequences of the colchicine-based in vivo autophagic flux assay

“…Beyond a simple ER-stress marker, Ddit3 encodes a ubiquitous transcription factor that is one of the most important components in the network of stress-inducible transcription. [33][34][35][36][37] In this regard, recent studies have identified DDIT3 as a direct regulator of numerous genes involved in the autophagic process. [38][39][40] Here, we show that the increase of transcripts levels of several autophagy-and lysosome-related genes in colchicinetreated fish paralleled that of Ddit3 mRNA supporting these previous studies.…”