(C-A) and (G-A) anchored simple sequence repeats (ASSRs) generated polymorphism in soybean, Glycine max (L.) Merr.

Wang, G.; Mahalingam, Ramamurthy; Knap, Halina T.

doi:10.1007/s001220050843

Cited by 35 publications

(15 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sixty-five ISSR fragments were scored for segregation: 60 were in testcross configuration and five in intercross configuration ( Table 1). As also found by Fang et al (1997) and Wang et al (1998) all (AT) n primers failed to give amplification products. Table 2 shows the 22 ISSR primers used, the annealing temperature and the number of polymorphic bands detected per primer.…”

Section: Rapd and Issrmentioning

confidence: 86%

A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers

Casasoli¹,

Mattioni²,

Cherubini³

et al. 2001

Theor Appl Genet

100

View full text Add to dashboard Cite

A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers was constructed using the two-way pseudo-testcross strategy. A total of 96 individuals from a F 1 full-sib family was genotyped with 381 molecular markers (311 RAPDs, 65 ISSRs, 5 isozymes). Markers in testcross configuration, segregating 1:1, were used to establish two separate maternal and paternal maps including 187 and 148 markers, respectively. The markers identified 12 linkage groups based on the haploid number of chestnut. The female and male framework maps reached a total length of 720 and 721 cM (Kosambi), respectively, representing a 76% and 68% coverage of the overall genome. A total of 46 markers, found in intercross configuration, segregating 3:1 and 1:2:1, were used to identify homologous linkage groups between parental maps; out of 12 linkage groups 11 could be joined. RAPD and ISSR markers showed a good and comparable reliability, allowing for the first time the establishment of a saturated linkage map for European chestnut. These maps will be a starting point for studies on the structure, evolution and function of the chestnut genome. Identification of QTLs for adaptive traits in chestnut will be the primary target.

show abstract

Section: Rapd and Issrmentioning

confidence: 86%

A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers

Casasoli¹,

Mattioni²,

Cherubini³

et al. 2001

Theor Appl Genet

100

View full text Add to dashboard Cite

show abstract

“…Joshi et al 2000, Kochieva et al 2002. One possibility is that unsuccessful amplifications could be due to self-complementarities of (AT) repeats, which may self-anneal and form dimers and hairpin loops, hindering amplification (Wang et al 1998, Cekic et al 2001. Arnaud-Haond et al (2005) compared genetic polymorphism using the same 2 sets of SSR markers utilized here.…”

Section: Discussionmentioning

confidence: 99%

Comparison of ISSR and SSR markers for analysis of genetic diversity in the seagrass Posidonia oceanica

Serra¹,

Procaccini²,

Intrieri³

et al. 2007

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Posidonia oceanica is an endemic seagrass species in the Mediterranean Sea. Due to its ecological importance, it has been the subject of several genetic studies aimed at increasing general knowledge of the system and helping to define appropriate management strategies. We compared the efficiency of 2 different classes of molecular markers, 'inter simple sequence repeats' (ISSRs) and 'simple sequence repeats' (SSRs) or 'microsatellites', in population genetic analysis of P. oceanica. To do this, we analyzed meadows along the coasts of the Calabria peninsula (Italy, South Tyrrhenian Sea and Ionian Sea), a putative biogeographic barrier for P. oceanica. SSR and ISSR markers detected different levels of genetic variability within P. oceanica populations. Dinucleotide microsatellites were more polymorphic than tri-and heptanucleotide microsatellites and ISSRs. Nevertheless, discriminating power analysis performed for each single locus showed that some trinucleotide microsatellite and ISSR primers were more sensitive than dinucleotides. The use of selected ISSR and SSR markers together allowed the detection of higher polymorphism than either set of marker alone. Tyrrhenian meadows were always more polymorphic than Ionian ones, according to both SSRs and ISSRs. Both markers strongly suggested the Calabria peninsula as an important biogeographic barrier between the Western and the Eastern side of the Mediterranean basin. KEY WORDS: Posidonia oceanica · ISSR · SSR · Genetic diversity · Biogeographic barrierResale or republication not permitted without written consent of the publisher

show abstract

“…By contrast researches employing the ISSR technique have mainly focused on cultivated species (Moreno et al 1998;Wang et al 1998;Blair et al 1999). However few recent papers have demonstrated the high potential of these markers for population and species-level studies (Esselman et al 1999;Ge and Sun 1999;Clausing et al 2000;Joshi et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Comparison of ISSR and RAPD markers to characterize three Chilean Nothofagus species

Mattioni

Casasoli

González³

et al. 2002

Theor Appl Genet

View full text Add to dashboard Cite

The present study is the first report of fingerprinting on three Chilean Nothofagus species using ISSR and RAPD markers; 61 Nothofagus nervosa, 32 Nothofagus obliqua and 32 Nothafagus dombeyi individual trees, sampled from collection and natural sites, were analyzed. Among 45 primers tested, the 6 ISSR and 6 RAPD primers selected for the analysis generated a total of 63 ISSR and 42 RAPD fragments. A high proportion of polymorphic bands, ranging from 97% and 80%, was found using both markers. A similar number of private and marker bands was generated by both markers in all the species examined and one discriminant ISSR fragment was obtained for N. dombeyi. Jaccard and Dice similarity indices were used to evaluate pairwise genetic divergence; cluster analysis of the similarity matrices was performed to estimate the intra- and inter-specific genetic diversity, and PCA analysis was employed to evaluate the resolving power of the markers to differentiate between the species. These analyses, carried out for both markers, allowed us to identify three main groups corresponding to the three Nothofagus species. The results of the present study can be seen as a starting point for future researches on the population and evolutionary genetics of these species.

show abstract

(C-A) and (G-A) anchored simple sequence repeats (ASSRs) generated polymorphism in soybean, Glycine max (L.) Merr.

Cited by 35 publications

References 25 publications

A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers

A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers

Comparison of ISSR and SSR markers for analysis of genetic diversity in the seagrass Posidonia oceanica

Comparison of ISSR and RAPD markers to characterize three Chilean Nothofagus species

Contact Info

Product

Resources

About