C-26 and C-30 Apocarotenoids from Seeds of Ditaxis heterantha with Antioxidant Activity and Protection against DNA Oxidative Damage

Méndez-Robles, María Dolores; Permady, Herry Heriyati; Jaramillo-Flores, M. E.; Lugo‐Cervantes, Eugenia; Cardador‐Martínez, Anaberta; Canales-Aguirre, Alejandro A.; López-Dellamary, Fernando; Cerda-Garcı́a-Rojas, Carlos M.; Tamariz, Joaquı́n

doi:10.1021/np050489f

Cited by 23 publications

(15 citation statements)

References 28 publications

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“…Only a-tocopherol, BHA and BHT showed reactions. Mendez-Robles et al (2006) detected lower DPPH Å scavenging activities for two apocarotenoids from b-carotene (ditaxin, heteranthin) compared to b-carotene and lycopene. The reason for the measurable, but low DPPH Å scavenging activities could be seen in the concentrations used, which ranged in high non-physiological doses up to 1500 lM in methanol.…”

Section: Dpph å Scavenging Assaymentioning

confidence: 88%

Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay

2011

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Section: Dpph å Scavenging Assaymentioning

confidence: 88%

Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay

2011

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“…Early studies realized on Ditaxis heterantha seeds were on plant distribution in Mexico, pigment identification and aroma compound production by enzymatic hydrolysis from the carotenoid oleoresin (Méndez‐Robles et al. , ; Del Toro‐Sánchez et al. ).…”

Section: Introductionmentioning

confidence: 99%

Functional properties of Ditaxis heterantha proteins

Espino‐Sevilla

Jaramillo-Flores

Hernández‐Gutiérrez

et al. 2013

Food Science & Nutrition

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Ditaxis heterantha is a plant of the Euphorbiaceae family that grows in semiarid regions of Mexico. It produces yellow pigmented seeds that are used for coloring of foods. The seeds contain about 20% of proteins. Proteins of D. heterantha were extracted and fractionated on the basis of solubility. Three main protein fractions were obtained: glutelins, 488 ± 0.5; albumins, 229 ± 2; and total globulins, 160 ± 1 g/kg. The amino acid profile was evaluated for each fraction and protein isolated, where the protein isolate contains essential amino acids such as Val, Phe, Tyr, and Leu. A calorimetric study showed that globulins and glutelins have a high denaturing temperature between 100 and 106°C, while albumins showed a denaturing temperature at 76°C. The protein isolate and its fractions exhibited functional properties: the isolated protein demonstrated good oil‐holding capacity of 40.7 g/kg. Foam capacity (FC) and foam stability (FS) were observed principally in glutelins and globulins where FC maximum was 330% and the FS was 28 min. The emulsifying capacity was observed in the same fractions of glutelins and globulins, followed by albumins. However, the glutelin fraction in particular was the only fraction that exhibited emulsifying stability at pH 5, 6, and 7. Gelling capacity was observed in albumins and globulins. This study indicated that protein isolated from D. heterantha could be used in food formulations due to its essential amino acid profile. Glutelin could be used as an emulsifying additive. Additionally, glutelin and globulin were stable at temperatures above 100°C; this is an important factor in food industry, principally in heat processes.

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“…The slides were then placed into a lysis solution (2.5 M NaCl, 100 mM Na 2 EDTA, 10 mM Tris-base, 35 mM sodium N-lauroylsarcosine, 0.2 M NaOH, and 20 % DMSO, pH = 10) at 4°C for 2 h. Then the slides were washed with ultrapure water three times and placed into a horizontal gel electrophoresis tank filled with fresh alkaline buffer (300 mM NaOH and 1 mM Na 2 EDTA, pH = 13) to unwind DNA for 40 min at 4°C. Following this procedure, electrophoresis was performed for 20 min under 27 V. The slides were finally washed with neutralizing buffer (400 mM Tris-base, pH = 7.5) and then stained with etidium bromide [13]. Valuable data were acquired using a CASP1.22 image analysis system attached to an OLYMPUS CX41 fluorescence microscope and selected randomly for each condition.…”

Section: Comet Assaymentioning

confidence: 99%

Lignans from the Flower Buds ofMagnolia lilifloraDesr.

Wang¹,

Lan²,

Wu³

et al. 2011

Planta Med

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Six new lignans, 1- 6, along with six known compounds were obtained from the flower buds of Magnolia liliflora Desr. The new lignans were elucidated as (1 S*,2 R*,5 S*,6 S*)-2-(3,5-dimethoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (1), (1 R*,2 R*,5 R*,6 S*)-2-(3,5-dimethoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (2), (1 R*, 2 R*,5 R*,6 S*)-2,6-bis (3,5-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (3), (1 R*,2 S*,5 R*,6 R*)-2-(3,4-methylenedioxyphenyl)-6-(3,5-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane ( 4), (7' S*,8 R*,8' R*)-3,5'-dimethoxy-3',4,9'-trihydroxy-7',9-epoxy-8,8'-lignan (5), and (7' R*,8' S*)-3,3',4,5'-tetramethoxy-7-en-7',9-epoxy-8,8'-lignan (6), by the analysis of 1D and 2D-NMR as well as HRESIMS data. The capacity of compound 1 to protect against damages to the DNA of rat lymphocyte cells induced by UV irradiation was assessed by the comet assay. It showed stronger antigenotoxicity than ascorbic acid from 6×10(-3) mmol·L(-1) to 6×10(-6) mmol·L(-1).

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C-26 and C-30 Apocarotenoids from Seeds of Ditaxis heterantha with Antioxidant Activity and Protection against DNA Oxidative Damage

Cited by 23 publications

References 28 publications

Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay

Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay

Functional properties of Ditaxis heterantha proteins

Lignans from the Flower Buds ofMagnolia lilifloraDesr.

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