BZR1 and BES1 participate in regulation of glucosinolate biosynthesis by brassinosteroids in Arabidopsis

Abstract

The effect of 24-epibrassinolide (EBR) on glucosinolate biosynthesis in Arabidopsis thaliana was investigated in the present study by using mutants and transgenic plants involved in brassinosteroid (BR) biosynthesis and signal transduction, as well as glucosinolate biosynthesis. The results showed that EBR significantly decreased the contents of major aliphatic glucosinolates including glucoiberin (S3), glucoraphanin (S4), and glucoerucin (T4), as well as the indolic glucosinolates glucobrassicin (IM) and neoglucobrassicin (1IM). In addition, a significantly higher level of glucosinolates accumulated in the BR-deficient mutant cpd and a dramatically lower glucosinolate content in the transgenic plant DWF4-ox overexpressing the BR biosynthetic gene DWF4 compared with their related wild-types, confirmed the repressing effect of BR on glucosinolate biosynthesis. BRI1, the receptor of BR signal transduction, was involved in regulation of glucosinolate biosynthesis by BR. Furthermore, the observation of reduced content of glucosinolates and lower expression levels of glucosinolate biosynthetic genes in 35S-BZR1/bzr1-1D and bes1-D plants compared with the corresponding wild-types suggested that BZR1 and BES1, two important components in BR signal transduction, are responsible for the inhibiting role of BR in glucosinolate biosynthesis. The disappearance of the repressing effect of BR on glucosinolate content in the myb28, myb34, and myb122 mutants indicated that these three MYB factors are important for the regulation of BR in glucosinolate biosynthesis.

Effects of glucose and gibberellic acid on glucosinolate content and antioxidant properties of Chinese kale sprouts

“…A number of studies have been conducted with the aim of improving these properties. Chemical regulation was one of the most studied methods, especially with phytohormones, which have been reported to play important roles in the regulation of glucosinolate biosynthesis (Yuan et al, 2010a;Guo et al, 2013a;Kim et al, 2013;Schweizer et al, 2013;Frerigmann and Gigolashvili, 2014;Zang et al, 2015). Our previous research indicated that 5% (0.05 g/ml) glucose elevated glucosinolate in Chinese kale and pak choi sprouts (Wei et al, 2011) and the study by Huang et al (2014) showed a promoting effect of 100 μmol/L GA 3 on glucosinolate content in the siliques of oilseed rape.…”

“…The glucosinolate content varies depending on genetic and environmental factors, and is modulated by external signals such as sugar (Miao et al, 2013) and phytohormones (Yuan et al, 2009;Sun et al, 2012;Wang et al, 2012;Guo et al, 2013a;2013b;Huseby et al, 2013;Kim et al, 2013;Zang et al, 2015). Glucose is not only the basal carbon and energy source but also a signaling molecule having regulatory effects throughout the life cycle of plant (Sheen, 2014).…”

Transcriptome atlas of the Arabidopsis funiculus – a study of maternal seed subregions

“…It involves the integration of signals from sugars (Miao et al, 2013), brassinosteroids (Guo et al, 2013a), jasmonic acid (Guo et al, 2013b), salicylic acid (Mewis et al, 2005), ethylene, and auxin (Schweizer et al, 2013), in addition to a network of TFs (Gigolashvili et al, 2007;Malitsky et al, 2008;Sønderby et al, 2010). Our analysis has identified several TF as potential regulators of GS biosynthesis in the Arabidopsis funiculus.…”

Virulence Factors of Geminivirus Interact with MYC2 to Subvert Plant Resistance and Promote Vector Performance

“…GSs were extracted and analyzed as described previously (Guo et al, 2013). Leaf samples (200 mg) from the wild type (Col-0) or the transgenic bC1 expression line (bC1-1/At) were used.…”