“…The first polyclonal antibody generated by our laboratory, D033, revealed expression in the proepicardium, migrating epicardium, epicardial-derived mesenchyme, and smooth muscle cells of the cardiac arteries of the developing chicken heart . A second polyclonal antibody, B846, also revealed Bves expression in cardiac muscle and all epicardial/epicardially derived tissues listed above (Wada et al, 2001), as well as expression in various epithelial cell lines (Wada et al, 2001), epithelia of all three germ layers during early chick development, epidermis, gut endoderm (Osler and Bader, 2004), and epithelia of the lens, retina, and cornea (Ripley et al, 2004). A subsequent antibody against the X. laevis ortholog of Bves was developed, and has revealed highly similar expression in the frog (Ripley et al, 2006).…”