Bves expression during avian embryogenesis

Osler, Megan E.; Bader, David M.

doi:10.1002/dvdy.10490

Cited by 23 publications

(52 citation statements)

References 26 publications

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“…2D). These patterns are in general agreement with the subcellular distribution detected with polyclonal reagents Wada et al, 2001;Osler and Bader, 2004). Therefore, the SB monoclonal series recognizes Bves in a variety of cell types across a spectrum of mammalian species and cell lines, in agreement with previously generated reagents.…”

Section: ␣-Bves Monoclonal Antibodies Detect Bves In Epithelial Cell supporting

confidence: 85%

“…Analyses of Bves expression in the chicken utilizing in situ hybridization techniques differ from results obtained using immunochemical methods Andree et al, 2000;Wada et al, 2001;Osler and Bader, 2004;Vasavada et al, 2004). While both methods provide evidence for expression in muscle cell types, in situ hybridization assays and immunochemical assays do not agree concerning expression in epithelial cell types.…”

Section: ␣-Bves Monoclonal Antibodies Detect Bves In Epithelial Cell mentioning

confidence: 97%

“…Immunochemical reagents against mouse and chicken Bves have previously been generated and characterized DiAngelo et al, 2001;Wada et al, 2001;Osler and Bader, 2004;Ripley et al, 2004;Vasavada et al, 2004). Recently, DiAngelo et al (2001) have generated a monoclonal antibody against the intracellular C-terminus of chicken Bves.…”

Section: ␣-Bves Monoclonal Antibodies Recognize Bves Specificallymentioning

confidence: 99%

“…The first polyclonal antibody generated by our laboratory, D033, revealed expression in the proepicardium, migrating epicardium, epicardial-derived mesenchyme, and smooth muscle cells of the cardiac arteries of the developing chicken heart . A second polyclonal antibody, B846, also revealed Bves expression in cardiac muscle and all epicardial/epicardially derived tissues listed above (Wada et al, 2001), as well as expression in various epithelial cell lines (Wada et al, 2001), epithelia of all three germ layers during early chick development, epidermis, gut endoderm (Osler and Bader, 2004), and epithelia of the lens, retina, and cornea (Ripley et al, 2004). A subsequent antibody against the X. laevis ortholog of Bves was developed, and has revealed highly similar expression in the frog (Ripley et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Detection of Bves message through in situ hybridization, Northern blotting (Andree et al, 2000), or lacZ knock-in (Andree et al, 2002a) do not agree with detection of the Bves protein using multiple antiBves immunological reagents DiAngelo et al, 2001;Wada et al, 2001;Osler and Bader, 2004;Ripley et al, 2004;Vasavada et al, 2004). While in situ hybridization and lacZ knock-in analyses have been interpreted as indicating that Bves is expressed preferentially in cardiac and skeletal muscle, analyses of protein expression indicate that Bves is expressed in many epithelial cell types as well.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Bves expression during mouse development using newly generated immunoreagents

Smith

Bader

2006

Dev. Dyn.

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Bves (blood vessel/epicardial substance) is a transmembrane protein postulated to play a role in cell-cell interaction/adhesion. It was independently isolated by two groups as a gene product highly enriched in the developing heart. Disagreement exists about its expression during development. Most notably, the expression of Bves in non-muscle cells is disputed. Determining the expression profile of Bves is a critical initial step preceding the characterization of protein function in development and in the adult. We have generated new monoclonal antibodies against mouse Bves and used these immunoreagents to elucidate Bves expression in development. As expected, we detect Bves in myocytes of the developing heart throughout development. In addition, skeletal and smooth muscle cells including those of the coronary system express Bves. Finally, specific, but not all, epithelial derivatives of the three germ layers are stained positively with these monoclonal antibodies. Protein expression in cultured epithelial and muscle cell lines corroborate our in vivo findings. Taken together, these results demonstrate the expression of Bves in a wide range of epithelial and muscle cells during mouse embryogenesis and indicate a broad function for this protein in development, and show that these newly generated reagents will be invaluable in further investigation of Bves.

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Section: ␣-Bves Monoclonal Antibodies Detect Bves In Epithelial Cell supporting

confidence: 85%

Section: ␣-Bves Monoclonal Antibodies Detect Bves In Epithelial Cell mentioning

confidence: 97%

Section: ␣-Bves Monoclonal Antibodies Recognize Bves Specificallymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of Bves expression during mouse development using newly generated immunoreagents

Smith

Bader

2006

Dev. Dyn.

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Glass transition and mechanical properties of PLLA and PDLLA‐PGA copolymer blends

Nakafuku

Takehisa

2004

J of Applied Polymer Sci

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ABSTRACT:The change of the glass transition temperatures (T g ) in the blend of poly(l-lactic acid) (PLLA) and the copolymers of poly(D,l-lactic acid) and poly(glycolic acid) (PDLLA-PGA) with different D,l-lactic acid and glycolic acid composition ratio (50 : 50, 65 : 35, and 75 : 25) was studied by DSC. Dynamic mechanical measurement and tensile testing were performed at various temperatures around T g of the blend. In the blend of PLLA and PDLLA-PGA50 (composition ratio of PDLLA and PGA 50 : 50), T g decreased from that of PLLA (about 58°C) to that of PDLLA-PGA50 (about 30°C). A single step decrease was observed in the DSC curve around T g between the weight fraction of PLLA (W(PLLA)) 1.0 and 0.7 (about 52°C) but two-step changes in the curve are observed between W(PLLA) ϭ 0.6 and 0.3. The T g change between that of PLLA and that of PDLLA-PGA and the appearance of two T g s suggest the existence of PLLA rich amorphous region and PDLLA-PGA copolymer rich amorphous region in the blend. A single step decrease of EЈ occurs at around T g of the pure PLLA but the two-step decrease was observed at W(PLLA) ϭ 0.6 and 0.4, supporting the existence of the PLLA rich region and PDLLA-PGA rich region. Tensile testing for various blends at elevated temperature showed that the extension without yielding occurred above T g of the blend. Partial miscibility is suggested for PLLA and PDLLA-PGA copolymer blends.

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Comparative analysis of mRNA and protein expression of Popdc1 (Bves) during early development in the chick embryo†

Torlopp

Breher

Schlüter

et al. 2006

Developmental Dynamics

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The isolation of the Popeye gene family was based on its preferential expression in striated muscle tissue. Recently, a monoclonal antibody against chick Popdc1 (also known as Bves) became available and was used in this study to comparatively analyze the expression pattern of Popdc1 at both the protein and mRNA level during early chick embryogenesis. Using whole-mount immunohistochemistry, expression in the heart was first observed at Hamburger and Hamilton (HH) stage 10 in the presumptive left ventricular segment. Cardiac expression was confined to differentiated cardiac myocytes, and undifferentiated myocytes at the anterior and posterior pole showed little expression. After looping, the outer curvature myocardium showed prominent Popdc1 staining, whereas the inner curvature was unlabeled. Despite previous reports, Popdc1 protein was not detectable at any time point in the proepicardium, epicardium, or the smooth muscle layer of the coronary vessels. Whole-mount in situ hybridization using a full-length Popdc1 probe detected novel expression domains, which have not been described previously. Popdc1 mRNA was found in Hensen's node at HH stage 4, and by HH stage 5؉, expression became asymmetric. In addition, Popdc1 mRNA was found in pharyngeal endoderm and in the notochordal plate. Subsequently, beginning at HH stage 9, Popdc1 mRNA expression was found in the cardiac mesoderm and expression was maintained in the heart in a pattern very similar to the one observed by antibody staining. Developmental Dynamics 235: 691-700, 2006.

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Bves expression during avian embryogenesis

Cited by 23 publications

References 26 publications

Characterization of Bves expression during mouse development using newly generated immunoreagents

Characterization of Bves expression during mouse development using newly generated immunoreagents

Glass transition and mechanical properties of PLLA and PDLLA‐PGA copolymer blends

Comparative analysis of mRNA and protein expression of Popdc1 (Bves) during early development in the chick embryo†

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