Buyang Huanwu Decoction promotes growth and differentiation of neural progenitor cells: Using a serum pharmacological method

Sun, Jinhao; Bi, Yushun; Guo, Lihua; Qi, Xin; Zhang, Jing; Li, Guibao; Tian, Guangdong; Ren, Fuxin; Li, Zhenhua

doi:10.1016/j.jep.2007.05.018

Cited by 38 publications

(24 citation statements)

References 12 publications

(13 reference statements)

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“…But the mechanism of its neuroprotection is not clear. Previous reports indicated that the neuroprotective effects of BYHWD may be associated with stimulation of neurogenesis, 21,22,[30][31][32] inhibition of apoptosis, 17,20 and activation of blood circulation. 19,33 Cai and Tong have reported that BYHWD could promote proliferation of the NPCs at the hippocampus and SVZ in the ischemic brains.…”

Section: Discussionmentioning

confidence: 99%

“…22,31 Other research performed in vitro has suggested that BYHWD may promote growth and differentiation of cultured NPCs. 21,34 Most of the reports focus mainly on the proliferation and differentiation of NPCs. To further clarify the mechanisms of BYHWD action on stroke, we have demonstrated herein the role of BYHWD on migration of NPCs in MCAO rats.…”

Section: Discussionmentioning

confidence: 99%

“…14,15 A large number of studies have found its mechanisms on the treatment of stroke, including anti-inflammation, 16 anti-oxidation, [16][17][18] improvement of hemorheological disorders, 19 inhibition of neural apoptosis, 17,20 and others. Experimental reports show that BYHWD may promote growth and differentiation of NPCs 21,22 ; however, the role of BYHWD in migration of NPCs is still unclear.…”

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confidence: 99%

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Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Precursor Cells

Kong

Zhu

et al. 2014

Rejuvenation Research

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Buyang Huanwu Decoction (BYHWD) is a classic formula widely used for treating stroke-induced disability, the highest morbidity of neurological disorders in China. However, the mechanism of its neuroprotection has not been fully clarified. Previous reports indicated that BYHWD may promote growth and differentiation of neural precursor cells (NPCs). The present study focused on the effects of BYHWD on migration of NPCs in rats with middle cerebral artery occlusion (MCAO). Rats were treated with different doses of BYHWD (12 and 24 grams/ kg) from day 1 to day 21 after model building. BYHWD could increase the survival rate and decrease neurological scores and infarct volume as compared with the vehicle-treated MCAO rats. Moreover, BYHWD treatment significantly increased 5-bromo-2-deoxyuridine (BrdU)-positive cells in the subventricular zone (SVZ), subgranular zone (SGZ), and corpus striatum (CS) of the infarct brain. Interestingly, BYHWD could markedly enhance BrdU + /doublecortin + cells not only in the SVZ and SGZ but also in CS, by up-regulating the protein expression of migration activators, including stromal cell derived factor-1, CXC chemokine receptor 4, vascular endothelial growth factor, Reelin, and brain-derived neurotrophic factor in the ipsilateral infarct area after MCAO. In addition, BYHWD treatment was able to promote the neuronal differentiation, which was closely related to the migratory process of NPCs in MCAO rats. These findings offer evidence for the first time that BYHWD may exert its neuroprotective effects partially by promotion of NPCs migration to ischemic brain areas.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Precursor Cells

Kong

Zhu

et al. 2014

Rejuvenation Research

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“…Buyang Huanwu Decoction (BYHWD), originating from 'Yi Lin Gai Cuo', is a traditional Chinese herbal prescription that has been applied in the treatment of cerebral ischemia in China for hundreds of years (1). Systematic reviews and meta-analyses of BYHWD have demonstrated that BYHWD is generally safe and possesses substantial neuroprotective action in patients and various animal models of focal ischemia (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is difficult to use only specific components of the formula to investigate the protective role of BYHWD. However, BYHWD-containing serum (BYHWD-CS) has been developed and is generally accepted for use in traditional Chinese medicine studies (1).…”

Section: Introductionmentioning

confidence: 99%

Upregulation of glutamate metabolism by BYHWD in cultured astrocytes following oxygen-glucose deprivation/reoxygenation in part depends on the activation of p38 MAPK

Guan

Zhou

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Recent studies have demonstrated that Buyang Huanwu Decoction (BYHWD) decreased glutamate levels subsequent to cerebral ischemia. Glutamate transporter-1 (GLT-1) and glutamine synthetase (GS), which are located in astrocytes, mainly contribute to glutamate transportation, thus reducing glutamate concentration. BYHWD has previously been demonstrated to upregulate GLT-1 and GS following ischemia in vivo. However, whether BYHWD can directly influence astrocytic GLT-1/GS levels remains unknown. In the present study, the effect of BYHWD containing serum (BYHWD-CS) on GLT-1/GS levels in astrocytes following oxygen-glucose deprivation/reoxygenation (OGD/R) was investigated. The results revealed that BYHWD-CS enhanced the expression levels of GLT-1 and GS in cultured astrocytes, which reduced glutamate concentration in the culture medium. Meanwhile, increased p38 mitogen-activated protein kinase (p38 MAPK) was phosphorylated (activation form) by BYHWD-CS in cultured astrocytes, and the specific p38 inhibitor SB203580 blocked the increase of GLT-1/GS accompanied by decreased cell viability. Furthermore, SB203580 suppressed the effect of BYHWD-CS on the level of glial fibrillary acidic protein (an astrocytic marker), thus confirming that astrocytes are directly involved in the protective role of BYHWD after OGD/R. These findings suggest that BYHWD upregulates GLT-1 and GS via p38 MAPK activation, and protects cultured astrocytes from death caused by OGD/R (typical in vitro model), which complemented the role of astrocytes in the protective effect of BYHWD.

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Determination of paeoniflorin, calycosin‐7‐O‐β‐d‐glucoside, ononin, calycosin and formononetin in rat plasma after oral administration of Buyang Huanwu decoction for their pharmacokinetic study by liquid chromatography–mass spectrometry

Zhang

Yang

Lin

et al. 2010

Biomedical Chromatography

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The purpose of this study was to simultaneously investigate the pharmacokinetics of five bioactive compounds in rat plasma after oral administration of Buyang Huanwu decoction (BYHWD) using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The separations were performed on a Thermo Hypersil Gold C(18) analytical column (50 × 2.1 mm, 3 µm) with the column temperature kept at 30°C. The quantitative analysis was performed using a quadrupole mass spectrometer detector operated under selected ion monitoring mode. A linear gradient elution of A (0.1% formic acid solution) and B (100% acetonitrile) was used at a flow rate of 0.2 mL/min. The method was validated within the concentration ranges 1.8-450, 6.0-1500, 2.0-500, 1.2-300 and 1.2-150 ng/mL for paeoniflorin, calycosin-7-O-β-d-glucoside, ononin, calycosin and formononetin, respectively. The calibration curves were linear with correlation coefficients > 0.99. The lower limits of quantitations were < 6.0 ng/mL. The method was further applied to assess the pharmacokinetics of the five bioactive constituents of BYHWD in rat plasma.

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Buyang Huanwu Decoction promotes growth and differentiation of neural progenitor cells: Using a serum pharmacological method

Cited by 38 publications

References 12 publications

Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Precursor Cells

Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Precursor Cells

Upregulation of glutamate metabolism by BYHWD in cultured astrocytes following oxygen-glucose deprivation/reoxygenation in part depends on the activation of p38 MAPK

Determination of paeoniflorin, calycosin‐7‐O‐β‐d‐glucoside, ononin, calycosin and formononetin in rat plasma after oral administration of Buyang Huanwu decoction for their pharmacokinetic study by liquid chromatography–mass spectrometry

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