Buxuhuayu decoction accelerates angiogenesis by activating the PI3K-Akt-eNOS signalling pathway in a streptozotocin-induced diabetic ulcer rat model

Qu, Keshen; Cha, Huijung; Ru, Yi; Hua-fa, Que; Xing, Meng

doi:10.1016/j.jep.2021.113824

Cited by 15 publications

(14 citation statements)

References 45 publications

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“…Calycosin-7-glucoside, the main chemical component of Astragalus membranaceus, exerts numerous biological effects, such as anti-inflammatory, anti-tumor, anti-aging, antioxidant, and vasodilation-related effects [ 29 ]. Moreover, it can increase the antioxidant capacity of cells, reduce cellular oxidative stress damage, and alleviate IR [ 30 ]. Salvianolic acid B is a natural product purified from Salvia miltiorrhiza (a member of the plant family Labiatae) that possesses known antioxidant, anti-inflammatory, neuroprotective, and other pharmacological activities.…”

Section: Discussionmentioning

confidence: 99%

Decoding the chemical composition and pharmacological mechanisms of Jiedu Tongluo Tiaogan Formula using high-performance liquid chromatography coupled with network pharmacology-based investigation

Zhang

Piao

Jin

et al. 2021

Aging

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Section: Discussionmentioning

confidence: 99%

Decoding the chemical composition and pharmacological mechanisms of Jiedu Tongluo Tiaogan Formula using high-performance liquid chromatography coupled with network pharmacology-based investigation

Zhang

Piao

Jin

et al. 2021

Aging

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“…Western blotting experiments were performed to investigate the effects of CFAC-3 treatment (1.25 and 12.5 µg/mL) on the wound healing mechanism focusing on the protein expression and phosphorylation of PI3K and AKT of fibroblast cells. PI3K/AKT signalling pathway is a recognised pathway strongly associated with the formation of an epidermis barrier and wound healing as it regulates cell proliferation, differentiation, and migration, along with angiogenesis and metabolism (Hou et al, 2019;Qu et al, 2021). AKT is located as one of the target proteins downstream of PI3K.…”

Section: Wound Healing Mechanism Via Pi3k/akt Pathway Signallingin Vi...mentioning

confidence: 99%

“…4-hydroxylbenzylaldehyde treatment significantly increased phosphorylation of ERK and AKT in keratinocytes, leading to keratinocyte cell migration (Kang et al, 2017). Buxuahuayu decoction, which is a mixture of herbs containing polyphenols, was reported to regulate the level of nitric oxide (NO) in local wounds by activating the PI3K/Akt/eNOS signalling pathway when tested in vivo on rats with diabetic ulcers (Qu et al, 2021).…”

Section: Pi-alkyl Tyr126mentioning

confidence: 99%

Beyond conventional biomass valorisation: pyrolysis-derived products for biomedical applications

et al. 2022

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Biomass valorisation is conventionally associated with the production of green biofuels. However, this could extend beyond the conventional perception of biomass application into other domains such as medical sciences. Acid condensate (AC) obtained from pyrolysis promises a good potential for biomedical applications, notably for its antimicrobial, antioxidant, and anti-inflammatory properties. In this study, concentrated AC extract (CACE) obtained from microwave-assisted pyrolysis of palm kernel shells was fractionated, and the resulting fractions were pooled according to similar thin layer chromatography profiles into combined fractions (CFACs). CFACs were evaluated for total phenolic content, antioxidant level, cytotoxicity, and wound healing activities toward human skin fibroblast cells (HSF 1184). CFAC-3 showed the highest total phenolic content (624.98 ± 8.70 µg GAE/mg of sample) and antioxidant activities (DPPH IC50 of 29.47 ± 0.74 µg/mL, ABTS of 1247.13 ± 27.89 μg TE/mg sample, FRAP of 24.26 ± 0.71 mmol Fe(II)/mg sample, HFRS of 257.74 ± 1.74 µg/mL) compared to CACE (DPPH IC50 of 81.76 ± 2.81 µg/mL, ABTS of 816.95 ± 30.49 μg TE/mg sample, FRAP of 9.22 ± 0.66 mmol Fe(II)/mg sample, HFRS of 689.30 ± 36.00 µg/mL), no cytotoxic properties at ≤50 µg/mL, and significantly faster wound closure (at 1.25 µg/mL) compared to the control 12 h after treatment. The phosphorylation of the phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT) were upregulated, thus indicating that wound healing of CFAC-3 followed through this signalling pathway. To conclude, phenolic-rich CFAC-3 obtained from the pyrolysis of palm kernel shells demonstrated potential biomedical application as an alternative wound healing agent with high antioxidant and wound-healing activity. To the best of our knowledge, this was the first study to report on the wound healing activity of AC and its wound healing mechanism.

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“…Erectile dysfunction is one of the major complications of diabetes, occurring in about 50% of men with diabetes within ten years of diagnosis (114). Various factors contribute to diabetic erectile dysfunction (DED), including hyperglycemia, hypertension, hyperlipidemia, insulin resistance, androgen deficiency, and vascular and neuronal abnormalities (115,116). Mechanisms that mediate DED mainly include increased advanced glycation end products, impaired neuronal nitric oxide synthase (nNOS) synthesis, elevated levels of oxygen free radicals, and decreased levels of cyclic guanosine monophosphate (cGMP)-dependent kinase-1 and Nitric oxide (NO).…”

Section: Mesenchymal Stem Cell-derived Exosomes and Erectile Dysfunctionmentioning

confidence: 99%

The roles of mesenchymal stem cell-derived exosomes in diabetes mellitus and its related complications

Chen

2022

Front. Endocrinol.

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Diabetes mellitus is a type of metabolic disease characterized by hyperglycemia, primarily caused by defects in insulin secretion, insulin action, or both. Long-term chronic hyperglycemia can lead to diabetes-related complications, causing damage, dysfunction, and failure of different organs. However, traditional insulin and oral drug therapy can only treat the symptoms but not delay the progressive failure of pancreatic beta cells or prevent the emergence of diabetic complications. Mesenchymal stem cells have received extensive attention due to their strong immunoregulatory functions and regeneration effects. Mesenchymal stem cell-derived exosomes (MSC-Exos) have been proposed as a novel treatment for diabetic patients as they have demonstrated superior efficiency to mesenchymal stem cells. This review summarizes the therapeutic effects, mechanisms, challenges, and future prospects of MSC-Exos in treating diabetes mellitus and its related complications. This review supports the potential use of MSC-Exos in future regenerative medicine to overcome the current difficulties in clinical treatment, particularly in treating diabetes.

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Buxuhuayu decoction accelerates angiogenesis by activating the PI3K-Akt-eNOS signalling pathway in a streptozotocin-induced diabetic ulcer rat model

Cited by 15 publications

References 45 publications

Decoding the chemical composition and pharmacological mechanisms of Jiedu Tongluo Tiaogan Formula using high-performance liquid chromatography coupled with network pharmacology-based investigation

Decoding the chemical composition and pharmacological mechanisms of Jiedu Tongluo Tiaogan Formula using high-performance liquid chromatography coupled with network pharmacology-based investigation

Beyond conventional biomass valorisation: pyrolysis-derived products for biomedical applications

The roles of mesenchymal stem cell-derived exosomes in diabetes mellitus and its related complications

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