Butrylacetycholinesterase Activity in Liver and Plasma, Liver Glycogen and Plasma Glucose Content, Haematology and Behaviour of Clariid Catfish Clarias Gariepinus to Dichlorvos

Abstract

Semi-static bioassay experiment was conducted to ascertain Butrylacetycholinesterase enzyme activity in liver and plasma, liver glycogen and plasma glucose content, haematology as well as behaviour of Clarias gariepinus (mean weight 24.52±1.64 g) to varying concentrations (0, 0.4, 0.8, 1.6 mg L -1 ) of Dichlorvos for 96 hours. Butrylacetycholinesterase activity in liver was significantly (P <0.05) inhibited all through the exposure duration to 0.4, 0.8 and 1.6 mg L -1 of DDVP compared with control group of fish. Similarly, plasma BuChE activity was inhibited all through the exposure duration to 1.6 mg L -1 DDVP compared to control. There was a significant (P <0.05) inhibition in HB, RBC and MCV of exposed fish after 24,48, 72 and 96h to 1.6 mg L -1 DDVP compared to the control but PCV and WBC were significantly elevated after 72 and 96h to 1.6 mg L -1 DDVP compared to the control.Plasma cortisol and liver glycogen were significantly inhibited after 72 and 96 h to 0.8 and 1.6 mg L

Modulations of blood biochemical parameters of golden mahseer, Tor putitora following exposures to single and mixed organophosphate

“…Significant elevation of blood glucose is due to gluconeogenesis which fuels the energy for the increased metabolic demands to cope with stressors including pesticides (Bhatnagar et al, 2017;Ramesh and Saravanan, 2008;Saravanan et al, 2011). Moreover, in previous studies depletion of liver glycogen in response to pesticide exposure was coupled with increased glucose in circulation, suggesting enhanced glycogenolysis as a strategy to meet the energy requirements (Ezike et al, 2017;Narra et al, 2015). In our experiment, glucose levels tended to be elevated by both single pesticide exposures, even though this was only significant for dichlorvos.…”

Haematology, Oxidative Stress and Micronuclei Frequency of Clarias Gariepinus Exposed to Glyphosate based Herbicide Glycot® GBHG

“…The study of the alteration in fish blood by herbicides and pesticides has been widely used by several workers to evaluate fish health status (Banaee et al, 2011;Ezike et al, 2017). The use of such haematological parameters such as packed cell volume PCV, numbers of red blood cells RBC and white blood cells WBC, haemoglobin HB and platelets PL has been noted to be indicators of toxicity with wide application in environmental monitoring and aquatic animal toxicological studies (Bacellos, 2003).…”

Polycyclic Aromatic Hydrocarbons (PAH) in Fingerlings of <em>Clarias gariepinus</em> (Burchell, 1822) to Petroleum

“…Due to the lipophilic nature and high chemical stability of PAHs, they accumulate in the fatty tissues of fish following their uptake. Fishes are therefore good indicators of pollution in inland and coastal waters [5]. Two broad groups exist based on their physical and biological properties including, high molecular weight (HMW) and low molecular weight (LMW) PAHs.…”