Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium

Reis, Verônica Massena; Santos, Paulina Estrada‐de los; Tenorio-Salgado, Silvia; Vogel, Jörg; Stoffels, Marion; Guyon, Soazig Le; Mavingui, Patrick; Baldani, V. L. D.; Schmid, Michael; Baldani, J. I.; Balandreau, J.; Hartmann, Anton; Caballero‐Mellado, Jesús

doi:10.1099/ijs.0.02879-0

Cited by 249 publications

(190 citation statements)

References 30 publications

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“…Escherichia coli strain E 2348/69 (Levine et al 1985) was from our culture collection. Bacteria were grown in liquid DYGS medium (Rodrigues Neto et al 1986) at 28 • C and shaken at 200 rpm for 24 h. Quantification of bacterial population in plant macerates was performed by colony forming units (CFU) on agar plates, using either DYGS medium or JMV medium (Reis et al 2004 Effects of the B. kururiensis colonization on rice growth were assessed in greenhouse conditions. Inoculated and non-inoculated plants were incubated and maintained under the same conditions as described above, harvested 120 dpi after planting for measurements of height, wet weight, determination of the number of stems and flowering and amount of seed production.…”

Section: B Kururiensis Strain Kp23mentioning

confidence: 99%

“…Unlike legume-nodulating symbionts, endophytically associated species have developed intimate and mutually beneficial interactions with different parts of the plants, colonizing roots, stems and leaves. Those include B. vietnamiensis (Gillis et al 1995, Govindarajan et al 2008, B. unamae (Caballero-Mellado et al 2004), B. tropica (Reis et al 2004), "B. brasilensis" (Baldani et al 1997), and B. phytofirmans . Plant-growth capabilities so far observed during endophytic associations suggest that the production of phytohormones and/or fixation of atmospheric nitrogen could act as positive regulators of mechanisms that lead to the plant development (Muñoz-Rojas andCaballero-Mellado 2003, Pedraza et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth

Mattos

Pádua

Romeiro

et al. 2008

An. Acad. Bras. Ciênc.

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Burkholderia kururiensis is a diazotrophic bacterium originally isolated from a polluted aquifer environment and presents a high level of similarity with the rice endophyte "B. brasilensis" species. This work assessed the ability of B. kururiensis to endophytically colonize rice plantlets by monitoring different tissues of root-inoculated plants for the presence of bacterial growth in different media, electron microscopy and by 16S rDNA analysis. Observations of roots, stems and leaves of inoculated rice plantlets by electron microscopy revealed B. kururiensis colonization predominantly on root hair zones, demonstrating endophytic colonization primarily through the endodermis, followed by spreading into xylem vessels, a possible pathway leading to aerial parts. Although indifferent for the bacterial growth itself, addition of a nitrogen source was a limiting factor for endophytic colonization. As endophytic colonization was directly associated to an enhanced plant development, production of phytohormone auxin/indole-3-acetic acid by B. kururiensis was assayed with transgenic rice plantlets containing an auxin-responsive reporter (DR5-GUS). Our findings suggest the ability of auxin production by plant-associated B. kururiensis which may have a stimulatory effect on plant development, as evidenced by activation of DR5-GUS. We hereby demonstrate, for the first time, the ability of B. kururiensis to endophytically colonize rice, promoting both plant growth and rice grain yield.

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Section: B Kururiensis Strain Kp23mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth

Mattos

Pádua

Romeiro

et al. 2008

An. Acad. Bras. Ciênc.

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“…Members of the genus Burkholderia have been found within many different ecological niches, but predominantly within the soil and the rhizosphere, from which some of the more recently described species have been isolated, such as Burkholderia sacchari (Brämer et al, 2001), Burkholderia tropica (Reis et al, 2004) and Burkholderia unamae (Caballero-Mellado et al, 2004).…”

mentioning

confidence: 99%

“…The type strain, FeGl01 T (=LMG 23612 T =CECT 7171 T =DSM 18251 T ), was isolated from ore material from the Jangada mine, Minas Gerais State, Brazil. (Reis et al, 2004); 5, B. silvatlantica (Perin et al, 2006); 6, B. mimosarum (Chen et al, 2006). +, Good growth; ±, poor growth; 2, no growth; V, variable; ND, no data available.…”

mentioning

confidence: 99%

Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil

Valverde

Delvasto

Peix

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, non-spore-forming bacterial strain with the ability to solubilize highly insoluble phosphatic minerals was isolated from a high-phosphorous iron ore from Minas Gerais State, Brazil. This strain, designated FeGl01T, was subjected to a polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis indicated that it formed a distinct phylogenetic lineage within the genus Burkholderia together with several other species of the genus, e.g. Burkholderia sacchari, Burkholderia tropica and Burkholderia unamae. Partial nucleotide sequencing and analysis of the recA gene roughly corroborated the phylogenetic position of strain FeGl01T within the genus Burkholderia. The chemotaxonomic properties of strain FeGl01T, such as ubiquinone Q-8 as the predominant quinone system and C16 : 0, C17 : 0 cyclo, C18 : 1 ω7c and C19 : 0 ω8c cyclo as the major fatty acids, were also consistent with its classification within the genus Burkholderia. DNA–DNA hybridization experiments between strain FeGl01T and the type strains of B. unamae, B. sacchari and B. tropica yielded reassociation values of 40 % or lower, which, together with qualitative and quantitative differences in fatty acid composition and with differences in several phenotypic traits, support the separation of the new isolate from the phylogenetically most closely related species. Therefore, it is suggested that strain FeGl01T represents a novel species of the genus Burkholderia, for which the name Burkholderia ferrariae sp. nov. is proposed. The type strain is FeGl01T (=LMG 23612T=CECT 7171T=DSM 18251T).

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“…isolates, identified as ARDRA genotypes 13, 14 and 15, showed almost-identical whole-cell protein patterns, as well as other common phenotypic and genomic features, such as nitrogenase activity with different carbon sources and identical nifHDK hybridization patterns. Very recently, N 2 -fixing isolates recovered from maize and teosinte plants grown in Mexico, as well as from sugarcane plants cultivated in Brazil and South Africa, were described as 'Burkholderia tropica' (Reis et al, 2004).…”

mentioning

confidence: 99%

Burkholderia unamae sp. nov., an N2-fixing rhizospheric and endophytic species

Caballero‐Mellado

Martínez‐Aguilar

Paredes-Valdez

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

184

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It was shown recently that the genus Burkholderia is rich in N2-fixing bacteria that are associated with plants. A group of these diazotrophic isolates with identical or very similar 16S rDNA restriction patterns [designated amplified rDNA restriction analysis (ARDRA) genotypes 13, 14 and 15] was selected and a polyphasic taxonomic study was performed, which included new isolates that were recovered from rhizospheres, rhizoplanes or internal tissues of maize, sugarcane and coffee plants. Morphological, physiological and biochemical features, as well as multi-locus enzyme electrophoresis profiles and whole-cell protein patterns, of 20 strains were analysed. In addition, analysis of cellular fatty acid profiles, 16S rDNA sequence analysis and DNA–DNA reassociation experiments were performed with representative strains. The taxonomic data indicated that the strains analysed belong to a novel diazotrophic Burkholderia species, for which the name Burkholderia unamae sp. nov. is proposed. Strain MTl-641T (=ATCC BAA-744T=CIP 107921T), isolated from the rhizosphere of maize, was designated as the type strain. B. unamae was found as an endophyte of plants grown in regions with climates ranging from semi-hot subhumid to hot humid, but not from plants grown in regions with semi-hot or hot dry climates. Moreover, B. unamae was isolated from rhizospheres and plants growing in soils with pH values in the range 4·5–7·1, but not from soils with pH values higher than 7·5.

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Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium

Cited by 249 publications

References 30 publications

Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth

Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth

Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil

Burkholderia unamae sp. nov., an N2-fixing rhizospheric and endophytic species

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