Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil

Abstract

A Gram-negative, non-spore-forming bacterial strain with the ability to solubilize highly insoluble phosphatic minerals was isolated from a high-phosphorous iron ore from Minas Gerais State, Brazil. This strain, designated FeGl01T, was subjected to a polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis indicated that it formed a distinct phylogenetic lineage within the genus Burkholderia together with several other species of the genus, e.g. Burkholderia sacchari, Burkholderia tropica and Burkholderia unamae. Partial nucleotide sequencing and analysis of the recA gene roughly corroborated the phylogenetic position of strain FeGl01T within the genus Burkholderia. The chemotaxonomic properties of strain FeGl01T, such as ubiquinone Q-8 as the predominant quinone system and C16 : 0, C17 : 0 cyclo, C18 : 1 ω7c and C19 : 0 ω8c cyclo as the major fatty acids, were also consistent with its classification within the genus Burkholderia. DNA–DNA hybridization experiments between strain FeGl01T and the type strains of B. unamae, B. sacchari and B. tropica yielded reassociation values of 40 % or lower, which, together with qualitative and quantitative differences in fatty acid composition and with differences in several phenotypic traits, support the separation of the new isolate from the phylogenetically most closely related species. Therefore, it is suggested that strain FeGl01T represents a novel species of the genus Burkholderia, for which the name Burkholderia ferrariae sp. nov. is proposed. The type strain is FeGl01T (=LMG 23612T=CECT 7171T=DSM 18251T).

The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing Burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation

“…These dissimilarities strongly contrast with the 100% identity found between phenol monooxygenase genes from B. kururiensis KP23 T and M130 isolated from two widely separated regions of the world. No less remarkable is the finding that B. tropica (54), B. sacchari (9), and B. ferrariae (72), all closely phylogenetically related to B. unamae, were unable to grow with the aromatic compounds benzene and phenol (data not shown). Nevertheless, very recently one B. tropica strain able to degrade benzene, toluene, and xylene was described (22), but this strain was identified only on the basis of PCR amplification and sequencing of 16S rRNA genes, which may be insufficient evidence to define its exact taxonomic position, considering that B. tropica and B. unamae show identity levels higher than 98% between their 16S rRNA gene sequences (11).…”

Suppression of Bacterial Wilt and Fusarium Wilt by a <i>Burkholderia nodosa</i> Strain Isolated from Kalimantan Soils, Indonesia

“…1). C17:0 cyclo is major fatty acid in B. ferrariae 38 , but not in B. nodosa 6) . Thus, the fatty acid profile of strain G5.2 was considered to be more similar to that of B. nodosa.…”

“…We checked by 16S rDNA sequences in 4 species but we did not sequence one isolate (G4.2), because in term of colony and substrate utilization of G4.1 and G4.2 isolates were similar. Fatty acid methyl ester profiles of strains C13.1, G4.1, G5.1 and G5.2 were compared with those of related Burkholderia species such us B. sacchari 4) , B. pyrrocinia 32) , B. terricola 11) , B. nodosa 6) , B. ferrariae 38) , B. unamae 5) and B. kururiensis 44) . The fatty acid profiles of strains C13.1, G4.1 and G5.1 were similar to those of B. sacchari, B. pyrrocinia and B. terricola, respectively.…”

Pathogenicity and biotechnological applications of the genus Burkholderia

“…Efficient phosphate-solubilizing bacteria could also be economically useful in emerging industrial activities such as biomining [40]; in particular, manufacturing of metallic iron and steel from iron ore resources requires the previous removal of phosphorus. The use of the novel species B. ferrariae, isolated from a high phosphorus iron ore and able to solubilise highly insoluble phosphatic minerals, could contribute to solve this problem in a cost-effective and environment-friendly way.…”