Burkholderia species in human infections in Mexico: Identification of B. cepacia, B. contaminans, B. multivorans, B. vietnamiensis,B. pseudomallei and a new Burkholderia species

Abstract

Background Burkholderia sensu stricto is comprised mainly of opportunistic pathogens. This group is widely distributed in the environment but is especially important in clinical settings. In Mexico, few species have been correctly identified among patients, most often B. cepacia is described. Methodology/Principal findings In this study, approximately 90 strains identified as B. cepacia with the VITEK2 system were isolated from two medical centers in Mexico City and analyzed by MLSA, BOX-PCR and genome analysis. The initial identification of B. cepacia was confirmed for many strains, but B. contaminans, B. multivorans and B. vietnamiensis were also identified among clinical strains for the first time in hospitals in Mexico. Additionally, the presence of B. pseudomallei was confirmed, and a novel species within the B. cepacia complex was documented. Several strains misidentified as B. cepacia actually belong to the genera Pseudomonas, Stenotrophomonas and Providencia. Conclusions/Significance The presence of different Burkholderia species in Mexico was confirmed. Correct identification of Burkholderia species is important to provide accurate treatment for immunosuppressed patients.

Identification of postharvest fruit biocontrol strain Burkholderia contaminans against fungi decay

“…known as Pseudomonas cepacia could cause decay of onion bulbs in 1950. Subsequently, several studies have reported this bacterium as an opportunistic human bacterial pathogen (Martina et al, 2018;Radilla et al, 2021). However, in agriculture, B. cepacia has multiple functions such as biological control, biodegradation, and promoting plant growth, showing its broader agricultural application pros-Cauduro been developed correspondingly with the advancements in molecular biology techniques.…”