Burkholderia orbicola sp. nov., a novel species within the Burkholderia cepacia complex

Morales-Ruíz, Leslie-Mariana; Rodríguez-Cisneros, Mariana; Kerber-Díaz, Jeniffer-Chris; Rojas-Rojas, Fernando Uriel; Ibarra, J. Antonio; Santos, Paulina Estrada‐de los

doi:10.1007/s00203-022-02778-0

Cited by 27 publications

(21 citation statements)

References 39 publications

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“…Burkholderia strains can synthesize these bacteriocins. B. cenocepacia AU1054 (now B. orbicola ) [ 11 ] produces an LlpA bacteriocin that inhibits B. ambifaria , Burkholderia anthina , B. cenocepacia , B. contaminans and Burkholderia metallica [ 73 ]. The homologue LlpA88 from B. orbicola TAtl-371 T inhibited the same species as strain AU1054 [ 66 ].…”

Section: Bacteriocinsmentioning

confidence: 99%

“…The Bcc species are best known as opportunistic pathogens, mainly in cystic fibrosis (CF) and immunocompromised patients [ 10 ]. Within the Bcc, the last species described was Burkholderia orbicola [ 11 ]. Another important feature within the Bcc is their resistance to many antibiotics [ 12 ], which especially endangers the lives of CF and immunocompromised patients.…”

Section: Introductionmentioning

confidence: 99%

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Compilation of the Antimicrobial Compounds Produced by Burkholderia Sensu Stricto

et al. 2023

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Due to the increase in multidrug-resistant microorganisms, the investigation of novel or more efficient antimicrobial compounds is essential. The World Health Organization issued a list of priority multidrug-resistant bacteria whose eradication will require new antibiotics. Among them, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae are in the “critical” (most urgent) category. As a result, major investigations are ongoing worldwide to discover new antimicrobial compounds. Burkholderia, specifically Burkholderia sensu stricto, is recognized as an antimicrobial-producing group of species. Highly dissimilar compounds are among the molecules produced by this genus, such as those that are unique to a particular strain (like compound CF66I produced by Burkholderia cepacia CF-66) or antimicrobials found in a number of species, e.g., phenazines or ornibactins. The compounds produced by Burkholderia include N-containing heterocycles, volatile organic compounds, polyenes, polyynes, siderophores, macrolides, bacteriocins, quinolones, and other not classified antimicrobials. Some of them might be candidates not only for antimicrobials for both bacteria and fungi, but also as anticancer or antitumor agents. Therefore, in this review, the wide range of antimicrobial compounds produced by Burkholderia is explored, focusing especially on those compounds that were tested in vitro for antimicrobial activity. In addition, information was gathered regarding novel compounds discovered by genome-guided approaches.

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Section: Bacteriocinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Compilation of the Antimicrobial Compounds Produced by Burkholderia Sensu Stricto

et al. 2023

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“…nov., closely related to Burkholderia cenocepacia (Wallner et al, 2019). A recent publication (Morales-Ruíz et al, 2022) updated our proposal to validate this separate species as B. orbicola , with TatI-371 as the type strain. This latter species name was thus retained in this article for ABIP444 (hereafter BoABIP444).…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics and transcriptomic response to root exudates of six rice root-associatedBurkholderia sensu latospecies

Wallner

Klonowska

Guigard

et al. 2022

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The rhizosphere can be described as the portion of soil under influence of plant roots through deposition and exudation of various molecules. It is a nutrient rich environment that is favorable for a multitude of soil-dwelling bacteria. Beyond being a reliable nutrient provider, some bacteria will perceive the plant as a potential host and undertake root colonization leading to mutualistic or parasitic interactions. Bacteria of the Burkholderia and Paraburkholderia genera are frequently found in the rhizosphere of rice. While the latter are often described as plant growth promoting species, Burkholderia are mostly studied for their human opportunistic traits. Here, we used root exudate stimulation on three Burkholderia and three Paraburkholderia strains isolated from rice roots to characterize their preliminary adaptation to the rice host at the transcriptomic level. Instead of the awaited genus-dependent adaptation, we observed a strongly species-specific response for all tested strains. While all bacteria originate from the rice environment, there are great disparities in their levels of adaptation following the sensing of root exudates. We further report the shared major functions that were differentially regulated in this early step of bacterial adaptation to plant colonization, including amino acids and putrescine metabolism, the Entner-Doudoroff (ED) pathway as well as cyclic diguanylate monophosphate (c-di-GMP) cycling.

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“…For example, the historical recA gene-based lineage originally identified in B. cenocepacia as III-B [11], was identified as a separate genomic taxa [12] and subsequently proposed as the new species B. orbicola sp. nov. [13]. B. gladioli , the third most common Burkholderia CF pathogen seen in the US [6] was thought to comprise several pathovars, but genomic analyses demonstrated that five distinct evolutionary clades existed within this single genomic species [21].…”

Section: Discussionmentioning

confidence: 99%

“…Burkholderia orbicola sp. nov. [13] has now been validly proposed as the species name for the genomic taxa represented by “Burkholderia servocepacia .” Overall, multiple studies have shown that epidemic and transmissible CF strains can be found in both B. cenocepacia and B. orbicola sp. nov. [9].…”

Section: Introductionmentioning

confidence: 99%

Identification of two distinct phylogenomic lineages and model strains for the understudied cystic fibrosis lung pathogenBurkholderia multivorans

Parfitt

Green

Connor

et al. 2023

Preprint

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Burkholderia multivoransis the dominantBurkholderiapathogen recovered from lung infection in people with cystic fibrosis. However, as an understudied pathogen there are knowledge gaps in relation to its population biology, phenotypic traits and useful model strains. A phylogenomic study ofB. multivoranswas undertaken using a total of 283 genomes, of which 73 were sequenced and 49 phenotypically characterized as part of this study. Average nucleotide identity analysis (ANI) and phylogenetic alignment of core genes demonstrated that theB. multivoranspopulation separated into two distinct evolutionary clades, defined as lineage 1 (n = 58 genomes) and lineage 2 (n = 221 genomes). To examine the population biology ofB. multivorans, a representative subgroup of 77B. multivoransgenomes (28 from the reference databases and the 49-novel short-read genome sequences) were selected based on multilocus sequence typing (MLST), isolation source and phylogenetic placement criteria. Comparative genomics was used to identifyB. multivoranslineage-specific genes:ghrB_1in lineage 1, andglnM_2in lineage 2, and diagnostic PCRs targeting them successfully developed. Phenotypic analysis of 49 representativeB. multivoransstrains showed considerable variance with the majority of isolates tested being motile and capable of biofilm formation. A striking absence ofB. multivoransprotease activityin vitrowas observed, but no lineage-specific phenotypic differences demonstrated. Using phylogenomic and phenotypic criteria, three modelB. multivoransCF strains were identified, BCC0084 (lineage 1), BCC1272 (lineage 2a) and BCC0033 lineage 2b, and their complete genome sequences determined.B. multivoransCF strains BCC0033 and BCC0084, and the environmental reference strain, ATCC 17616, were all capable of short-term survival within a murine lung infection model. By mapping the population biology, identifying lineage-specific PCRs and model strains, we provide much needed baseline resources for future studies ofB. multivorans.

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Burkholderia orbicola sp. nov., a novel species within the Burkholderia cepacia complex

Cited by 27 publications

References 39 publications

Compilation of the Antimicrobial Compounds Produced by Burkholderia Sensu Stricto

Compilation of the Antimicrobial Compounds Produced by Burkholderia Sensu Stricto

Comparative genomics and transcriptomic response to root exudates of six rice root-associatedBurkholderia sensu latospecies

Identification of two distinct phylogenomic lineages and model strains for the understudied cystic fibrosis lung pathogenBurkholderia multivorans

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