Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics

Lee, Song Hua; Chong, Chan-Eng; Lim, Boon San; Chai, San Jiun; Sam, Kin Kit; Mohamed, Rahmah; Nathan, Sheila

doi:10.1016/j.diagmicrobio.2007.01.002

Cited by 36 publications

(43 citation statements)

References 27 publications

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“…Three publications have investigated AMR in poultry pathogens, including Mycoplasma gallisepticum , Haemophilus paragallinarum , and Avibacterium paragallinarum [95,96,97], two in clinical and animal Burkholderia pseudomallei isolates [98,99], and one in Clostridium perfringens bacteria from diarrhoeic pigs [100]. …”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

et al. 2016

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Southeast Asia is an area of great economic dynamism. In recent years, it has experienced a rapid rise in the levels of animal product production and consumption. The region is considered to be a hotspot for infectious diseases and antimicrobial resistance (AMR). We reviewed English-language peer-reviewed publications related to antimicrobial usage (AMU) and AMR in animal production, as well as antimicrobial residues in meat and fish from 2000 to 2016, in the region. There is a paucity of data from most countries and for most bacterial pathogens. Most of the published work relates to non-typhoidal Salmonella (NTS), Escherichia coli (E. coli), and Campylobacter spp. (mainly from Vietnam and Thailand), Enterococcus spp. (Malaysia), and methicillin-resistant Staphylococcus aureus (MRSA) (Thailand). However, most studies used the disk diffusion method for antimicrobial susceptibility testing; breakpoints were interpreted using Clinical Standard Laboratory Institute (CSLI) guidelines. Statistical models integrating data from publications on AMR in NTS and E. coli studies show a higher overall prevalence of AMR in pig isolates, and an increase in levels of AMR over the years. AMU studies (mostly from Vietnam) indicate very high usage levels of most types of antimicrobials, including beta-lactams, aminoglycosides, macrolides, and quinolones. This review summarizes information about genetic determinants of resistance, most of which are transferrable (mostly plasmids and integrons). The data in this review provide a benchmark to help focus research and policies on AMU and AMR in the region.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

et al. 2016

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“…[10][11][12]35,[38][39][40] Even strains that originate from the same parental strain exhibit phenotypic diversity, particularly in colony morphology. 17,41 Both arginine deiminase and quorum sensing systems have been suggested to modulate the formation of colonies in response to different environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The observation that the B. pseudomallei phenotype and virulence were altered by growth in soil may partially explain the fact that B. pseudomallei isolated from different sources can exhibit dramatically different degrees of virulence. 31,39 It has been reported that B. pseudomallei isolates cultured from multiple sites of a single patient with disseminated melioidosis show rapid changes in genotypic characteristics. 49 Other studies have shown that the irreversible alteration of the colony morphology of B. pseudomallei isolated from a water environment was accompanied by changes in the genetic background.…”

Section: Discussionmentioning

confidence: 99%

Alteration of the Phenotypic and Pathogenic Patterns of Burkholderia pseudomallei that Persist in a Soil Environment

Chen

Shieh

Goldsmith³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Melioidosis is caused by the soil-borne pathogen Burkholderia pseudomallei. To investigate whether the distinct phenotypic and virulent characteristics result from environmental adaptations in the soil or from the host body, two pairs of isogenic strains were generated by passages in soil or mice. After cultivation in soil, the levels of 3-hydroxytetradecanoic acid, biofilm formation, flagellar expression, and ultrastructure were altered in the bacteria. Uniformly fatal melioidosis developed as a result of infection with mouse-derived strains; however, the survival rates of mice infected with soil-derived strains prolonged. After primary infection or reinfection with soil-derived strains, the mice developed a low degree of bacterial hepatitis and bacterial colonization in the liver and bone marrow compared with mice that were infected with isogenic or heterogenic mouse-derived strains. We suggest that specific phenotypic and pathogenic patterns can be induced through infection with B. pseudomallei that has been cultured in different (soil versus mouse) environments.

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“…15 Bacteria were grown in brain-heart infusion broth overnight at 37°. The cells were centrifuged at 10 000 g, suspended in brain-heart infusion broth containing 20% glycerol, frozen immediately in aliquots of 10 9 colony-forming units (CFU)/ml and stored at )80°.…”

Section: Bacteriamentioning

confidence: 99%

Delayed activation of host innate immune pathways in streptozotocin‐induced diabetic hosts leads to more severe disease during infection with Burkholderia pseudomallei

2012

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Summary Diabetes mellitus is a predisposing factor of melioidosis, contributing to higher mortality rates in diabetics infected with Burkholderia pseudomallei. To investigate how diabetes alters the inflammatory response, we established a streptozotocin (STZ) ‐induced diabetic murine acute‐phase melioidosis model. Viable B. pseudomallei cells were consistently detected in the blood, liver and spleen during the 42‐hr course of infection but the hyperglycaemic environment did not increase the bacterial burden. However, after 24 hr, granulocyte counts increased in response to infection, whereas blood glucose concentrations decreased over the course of infection. A genome‐wide expression analysis of the STZ‐diabetic murine acute melioidosis liver identified ∼ 1000 genes whose expression was altered in the STZ‐diabetic mice. The STZ‐diabetic host transcriptional response was compared with the normoglycaemic host transcriptional response recently reported by our group. The microarray data suggest that the presence of elevated glucose levels impairs the host innate immune system by delaying the identification and recognition of B. pseudomallei surface structures. Consequently, the host is unable to activate the appropriate innate immune response over time, which may explain the increased susceptibility to melioidosis in the STZ‐diabetic host. Nevertheless, a general ‘alarm signal’ of infection as well as defence programmes are still triggered by the STZ‐diabetic host, although only 24 hr after infection. In summary, this study demonstrates that in the face of a B. pseudomallei acute infection, poor glycaemic control impaired innate responses during the early stages of B. pseudomallei infection, contributing to the increased susceptibility of STZ‐induced diabetics to this fatal disease.

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Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics

Cited by 36 publications

References 27 publications

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

Alteration of the Phenotypic and Pathogenic Patterns of Burkholderia pseudomallei that Persist in a Soil Environment

Delayed activation of host innate immune pathways in streptozotocin‐induced diabetic hosts leads to more severe disease during infection with Burkholderia pseudomallei

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