Burkholderia gladioli MB39 an Antarctic Strain as a Biocontrol Agent

Sarli, Dinorah Anabella; Sánchez, Leandro Arturo; Delgado, Osvaldo Daniel

doi:10.1007/s00284-021-02492-y

Cited by 8 publications

(6 citation statements)

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“…In addition, S. proteamaculans 136 was previously reported associated with andrimid production, a potent nanomolar inhibitor pseudopeptide (Sánchez et al, 2013), supported by recent research (Fatiah et al, 2021). On the other hand, the antifungal activity of B. gladioli MB39 was recently described as being related to a mycophagy phenomenon (Sarli et al, 2021), consistent with data obtained in this study and a wide range of antifungal molecules of B. gladioli has also been recently reported (Elshafie et al, 2017; Esmaeel et al, 2017; Kumar et al, 2018; Kunakom & Eustáquio, 2019; Li et al, 2019). In addition, the production of gladiolin, toxoflavin, reumycin, enacyloxin and a putative new compound of molecular formula C 33 H 4 7Cl 2 NO 13 are different compounds produced by B. gladioli recently described by Depoorter et al (2021).…”

Section: Discussionsupporting

confidence: 91%

“…The three isolates have shown inhibitory activity against several phytopathogenic strains. Burkholderia gladioli MB39 (Sarli et al, 2021) and S. proteamaculans 137 (Table 3, Figure 2) have potent antifungal activity. Amongst the inhibited phytopathogenic fungi, the most sensitive species were Macrophomina phaseolina (the charcoal rot of soybean causative agent) and Penicillium digitatum .…”

Section: Resultsmentioning

confidence: 99%

“…Burkholderia gladioli MB39 and Serratia proteamaculans 136 were previously isolated and characterized (Sánchez et al, 2009; Sarli et al, 2021). In this study, Serratia proteamaculans 137 was grown at 15°C in LB medium containing g L −1 : yeast extract 5; tryptone 5; and NaCl 5 and B. gladioli MB39 was grown in PDB medium at 28°C.…”

Section: Materials and Methodologymentioning

confidence: 99%

“…Serratia proteamaculans 136, 137 and Burkholderia gladioli MB39 are natural antimicrobial producers isolated from sub-Antarctic and Antarctic environments (Danilovich et al, 2018;Sánchez et al, 2009Sánchez et al, , 2010Sarli et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Cold-adapted strains as plant growth-promoting bacteria on soybean seeds and biocontrol agents against Macrophomina phaseolina

Sarli

Gomes

Reznikov³

et al. 2022

Journal of Applied Microbiology

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Aim:The aim was to characterize cold-adapted bacteria by testing their PGP features and antagonistic activity against Macrophomina phaseolina, both in vitro and coating soybean seeds (Glycine max [L.] Merr.). Methods and results:Burkholderia gladioli MB39, Serratia proteamaculans 136 and Serratia proteamaculans 137 were evaluated. In vitro tests showed that S. proteamaculans 136 and 137 produce siderophore and indole-acetic acid (IAA), solubilize phosphate and fix nitrogen. Additionally, B. gladioli MB39 and S. proteamaculans 137 showed hydrolase activity and potent antifungal effects. The biocontrol efficacy over soybean seeds was evaluated using in vitro and greenhouse methods by immersing seeds into each bacterial suspension. As a result, S. proteamaculans 136 has improved the performance in all the seed germination evaluated parameters. In addition, S. proteamaculans 137 and B. gladioli MB39 strongly inhibited M. phaseolina, reducing the infection index values to 10% and 0%, respectively. Conclusion: Serratia proteamaculans 136, 137 and Burkholderia gladioli MB39 showed plant growth promotion features and inhibition of Macrophomina phaseolina infection by producing different antifungal compounds.Significance and impact of the study: Our results reinforce the application of cold-adapted Serratia proteamaculans and Burkholderia gladioli bacterial strains as candidates for developing microbial formulation to promote plant growth and guarantee antifungal protection in soybean crops.

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Materials and Methodologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cold-adapted strains as plant growth-promoting bacteria on soybean seeds and biocontrol agents against Macrophomina phaseolina

Sarli

Gomes

Reznikov³

et al. 2022

Journal of Applied Microbiology

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“…Members of the Bpc, and Burkholderia species in general, also can be sources of novel natural products (18,19). Indeed, Burkholderia species have been demonstrated to be useful for bioremediation (47,48), biocontrol (49), and as potential sources of novel antibiotics (50). The detailed genomic data generated in this study, and the deposition of the type strains in public strain collections, will hopefully facilitate detailed bioprospecting studies of B. mayonis sp.…”

Section: Genetic and Genomic Comparative Analysismentioning

confidence: 86%

Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.

Hall

Baker

Sahl

et al. 2022

Appl Environ Microbiol

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Distinct Burkholderia strains were isolated from soil samples collected in tropical northern Australia (Northern Territory and the Torres Strait Islands, Queensland). Phylogenetic analysis of 16S rRNA and whole genome sequences revealed these strains were distinct from previously described Burkholderia species and assigned them to two novel clades within the B. pseudomallei complex (Bpc). Because average nucleotide identity and digital DNA-DNA hybridization calculations are consistent with these clades representing distinct species, we propose the names Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Strains assigned to B. mayonis sp. nov. include type strain BDU6 T (=TSD-80; LMG 29941; ASM152374v2) and BDU8. Strains assigned to B. savannae sp. nov. include type strain MSMB266 T (=TSD-82; LMG 29940; ASM152444v2), MSMB852, BDU18, and BDU19. Comparative genomics revealed unique coding regions for both putative species, including clusters of orthologous genes associated with phage. Type strains of both B. mayonis sp. nov. and B. savannae sp. nov. yielded biochemical profiles distinct from each other and other species in the Bpc, and profiles also varied among strains within B. mayonis sp. nov. and B. savannae sp. nov. Matrix-assisted laser desorption ionization–time of flight analysis revealed a B. savannae sp. nov. cluster separate from other species, whereas B. mayonis sp. nov. strains did not form a distinct cluster. Neither B. mayonis sp. nov. nor B. savannae sp. nov. caused mortality in mice when delivered via the subcutaneous route. The addition of B. mayonis sp. nov. and B. savannae sp. nov. results in eight species currently in the Bpc. IMPORTANCE Burkholderia species can be important sources of novel natural products and new species are of interest to diverse scientific disciplines. Although many Burkholderia species are saprophytic, Burkholderia pseudomallei is the causative agent of the disease melioidosis. Understanding the genomics and virulence of the closest relatives to B. pseudomallei ( i.e., the other species within the Bpc) is important for identifying robust diagnostic targets specific to B. pseudomallei and understanding evolution of virulence in B. pseudomallei . Two proposed novel species, B. mayonis sp. nov. and B. savannae sp. nov., were isolated from soil samples collected from multiple locations in northern Australia. The two proposed species belong to the Bpc but are phylogenetically distinct from all other members of this complex. The addition of B. mayonis sp. nov. and B. savannae sp. nov. results in a total of eight species within this significant complex of bacteria that are available for future studies.

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