Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules

Abstract

Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia , with the representative strain WSM3556T being most closely related to Burkholderia caledonica LMG 23644T (98.70 % 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937T (98.50 %). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia . Results of DNA–DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain WSM3556T ( = LMG 27173T = HAMBI 3353T).

Burkholderia kirstenboschensis sp. nov. nodulates papilionoid legumes indigenous to South Africa

“…nov. isolates examined in this study. These 17 species include ecologically diverse taxa ranging from root-nodulaters (e.g., B. dilworthii and B. rhynchosiae) [23,24] and moss-associated species such as B.…”

“…All isolates displayed a positive reaction for the assimilation of adipic acid and a negative reaction for arginine dihydrolase which distinguished B. kirstenboschensis sp. nov. from B. rhynchosiae [24]. A positive reaction for all isolates for acetoin production and negative reactions for arginine dihydrolase, tryptophan deaminase and the oxidation of dilworthii, which appears to be the closest known relatives of B. kirstenboschensis sp.…”

“…The other four species also nodulate legumes in the subfamily Papilionoideae, where both B. sprentiae and B. dilworthii were isolated from Lebeckia ambigua [22,24], B. rhynchosiae originated from Rhynchosia ferulifolia [23] and B.…”

A large diversity of non-rhizobial endophytes found in legume root nodules in Flanders (Belgium)

“…While traditionally, rhizobia belonged to the genera, Azorhizobium, Bradyrhizobium, Ensifer, Mesorhizobium and Rhizobium (Sawada et al, 2003), in recent years nitrogen fixing root nodule bacteria have also been described in other Alphaproteobacterial genera, including Ochrobactrum (Trujillo et al, 2005), Methylobacterium (Sy et al, 2001), Microvirga (Ardley et al, 2012;Radl et al, 2014), Devosia (Rivas et al, 2003) and Phyllobacterium . Furthermore, so-called Betarhizobia have in the last ten years been described in the Betaproteobacterial genera Burkholderia and Cupriavidus (Chen et al, 2001;Moulin et al, 2001;De Meyer et al, 2013a;De Meyer et al, 2013b;De Meyer et al, 2014). In addition to strains that can elicit nodules and belong to documented rhizobial species, several other bacterial species have been reported from legume nodules without a clear indication of their role within the host.…”

Biogeographical Patterns of Legume-Nodulating Burkholderia spp.: from African Fynbos to Continental Scales

“…In South Africa, Burkholderia symbionts are widespread and associated with diverse lineages of the tribes Crotalarieae (23)(24)(25)(26), Hypocalypteae (27,28), Indigofereae (26), Phaseoleae (26,29,30), and Podalyrieae (26,27,31,32), indicating that the South African soils are an important reservoir for nodulating Burkholderia, and thus this needs to be explored further for new candidate species. With the exception of Burkholderia phymatum strains nodulating the nonnative crop species Phaseolus vulgaris (common bean) in Moroccan soils (33), the legume-Burkholderia symbiosis in Africa has only been reported in a range of sites within the fynbos region, supporting the idea of the Cape region as an exclusive biodiversity hot spot for the symbiosis (12).…”