Burkholderia kirstenboschensis sp. nov. nodulates papilionoid legumes indigenous to South Africa

Abstract

Despite the diversity of Burkholderia species known to nodulate legumes in introduced and native regions, relatively few taxa have been formally described. For example, the Cape Floristic Region of South Africa is thought to represent one of the major centres of diversity for the rhizobial members of Burkholderia, yet only five species have been described from legumes occurring in this region and numerous are still awaiting

Transfer of eleven species of the genus Burkholderia to the genus Paraburkholderia and proposal of Caballeronia gen. nov. to accommodate twelve species of the genera Burkholderia and Paraburkholderia

“…nov. The description is as provided by Steenkamp et al (2015) with the following additional properties. The position in phylogenetic trees based on 16S rRNA gene sequences and the specificities of conserved sequence indels are in accordance with the emended genus description.…”

“…Burkholderia jiangsuensis is placed in the same clade with Paraburkholderia grimmiae and Paraburkholderia zhejiangensis in phylogenetic trees based on the 16S rRNA, recA and gyrB gene sequences (Liu et al, 2014). The levels of similarity of the Burkholderia kirstenboschensis 16S rRNA gene sequence with the sequences of Paraburkholderia phytofirmans, Paraburkholderia fungorum, Paraburkholderia caledonica, Paraburkholderia bryophila, Paraburkholderia megapolitana and Paraburkholderia dilworthii were shown to be 98.5 % (Steenkamp et al, 2015). The nearest neighbour of this species in a phylogenetic tree reconstructed using concatenated 16S rRNA, atpD, recA and rpoB gene sequences was Paraburkholderia dilworthii (Steenkamp et al, 2015).…”

“…The levels of similarity of the Burkholderia kirstenboschensis 16S rRNA gene sequence with the sequences of Paraburkholderia phytofirmans, Paraburkholderia fungorum, Paraburkholderia caledonica, Paraburkholderia bryophila, Paraburkholderia megapolitana and Paraburkholderia dilworthii were shown to be 98.5 % (Steenkamp et al, 2015). The nearest neighbour of this species in a phylogenetic tree reconstructed using concatenated 16S rRNA, atpD, recA and rpoB gene sequences was Paraburkholderia dilworthii (Steenkamp et al, 2015). Phylogenetic analysis using 16S rRNA gene sequences showed that the closest relative of Burkholderia megalochromosomata is B. jiangsuensis, and both species were placed in a clade located near Paraburkholderia grimmiae and Paraburkholderia zhejiangensis (Baek et al, 2015a).…”

Biogeographical Patterns of Legume-Nodulating Burkholderia spp.: from African Fynbos to Continental Scales

“…In South Africa, Burkholderia symbionts are widespread and associated with diverse lineages of the tribes Crotalarieae (23)(24)(25)(26), Hypocalypteae (27,28), Indigofereae (26), Phaseoleae (26,29,30), and Podalyrieae (26,27,31,32), indicating that the South African soils are an important reservoir for nodulating Burkholderia, and thus this needs to be explored further for new candidate species. With the exception of Burkholderia phymatum strains nodulating the nonnative crop species Phaseolus vulgaris (common bean) in Moroccan soils (33), the legume-Burkholderia symbiosis in Africa has only been reported in a range of sites within the fynbos region, supporting the idea of the Cape region as an exclusive biodiversity hot spot for the symbiosis (12).…”

Does P deficiency affect nodule bacterial composition and N source utilization in a legume from nutrient-poor Mediterranean-type ecosystems?

“…The type strain information as well as links to the database accession numbers can be found on the List of Prokaryotic Names with Standing in Nomenclature (www.bacterio.net; Euzéby, 1997;Parte, 2013). There are however species included in the dataset which was not yet available on the list as of 14 March 2016, but for which published information is available B. metalliresistens (Guo et al, 2015), B. ginsengiterrae and B. panaciterrae (Farh et al, 2015) B. rinojensis (Cordova-Kreylos et al, 2013), B. kirstenboschensis (Steenkamp et al, 2015) and B. dipogonis (Sheu et al, 2015).…”